pSADdeltaG-GFP-AlstR
(Plasmid
#32647)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 32647 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepSADdeltaG
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Backbone manufacturerAddgene plasmid 32634
- Backbone size w/o insert (bp) 15428
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Vector typeMammalian Expression ; Rabies virus
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert 1
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Gene/Insert nameGFP
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Insert Size (bp)700
- Promoter CMV
Cloning Information for Gene/Insert 1
- Cloning method Restriction Enzyme
- 5′ cloning site NA (unknown if destroyed)
- 3′ cloning site NA (unknown if destroyed)
- 5′ sequencing primer CMV-F (Common Sequencing Primers)
Gene/Insert 2
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Gene/Insert nameAlstR
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Alt nameallatostatin receptor
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SpeciesD. melanogaster (fly)
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Insert Size (bp)1200
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Entrez GeneAstA-R1 (a.k.a. Dmel_CG2872, ASR, AST-R, AlstR, AstA R1, AstAR1, CG2872, D.AlstR1, DAR-1, DAR1, DGR, Dar-1, Dmel\CG2872, EG:121E7.2, GR)
Cloning Information for Gene/Insert 2
- Cloning method Restriction Enzyme
- 5′ cloning site NA (unknown if destroyed)
- 3′ cloning site NA (unknown if destroyed)
- 5′ sequencing primer CMV-F
- 3′ sequencing primer BGH-Rev (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSADdeltaG-GFP-AlstR was a gift from Edward Callaway (Addgene plasmid # 32647 ; http://n2t.net/addgene:32647 ; RRID:Addgene_32647) -
For your References section:
New rabies virus variants for monitoring and manipulating activity and gene expression in defined neural circuits. Osakada F, Mori T, Cetin AH, Marshel JH, Virgen B, Callaway EM. Neuron. 2011 Aug 25;71(4):617-31. 10.1016/j.neuron.2011.07.005 PubMed 21867879
Map uploaded by the depositor.