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Plasmid#193137PurposeExpresses dCas9 fused to the SunTag scaffoldDepositorInsertdCas9-SunTag-T2A-HygR
UseLentiviralTagsExpressionMutationPromoterPGKAvailable sinceNov. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKIIa-hM3D(Gq)-mCherry
Plasmid#50476PurposeGq-coupled hM3D DREADD fused with mCherry under the control of CaMKIIa promoterDepositorUseAAVTagsmCherryExpressionMutationSee supplemental documents for DREADD mutationsPromoterCaMKIIaAvailable sinceApril 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
mCherry-Myotilin-C-14
Plasmid#55109PurposeLocalization: Cytoskeleton, Excitation: 587, Emission: 610DepositorInsertMyotilin (MYOT Human)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceAug. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLS13-IL-10
Plasmid#209128PurposeTransient mammalian expression of IL-10DepositorInsertIL-10 (IL10 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-SYK
Plasmid#23907DepositorInsertSYK (SYK Human)
UseGateway donor vectorTagsExpressionMutationPromoterAvailable sinceJuly 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
pLenti-enCas12a-2xNLS
Plasmid#209021PurposeLentiviral vector expressing human codon-optimized enCas12a with 2xNLSDepositorInsertenCas12a-2xNLS-Myc-NeoR
UseCRISPR and LentiviralTagsMYCExpressionMammalianMutationE174R/S542R/K548RPromoterEF-1aAvailable sinceJune 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pM-Cas9
Plasmid#196325Purpose35S promoter-driven expression of the positive-sense TSWV M RNA segment encoding SpCas9 in place of viral GPDepositorInsertFull length TSWV M antigenome encoding SpCas9 in place of viral GP
UseCRISPRTags3×flagExpressionPlantMutationSee depositor comments belowPromoterduplicated cauliflower mosaic virus 35S promoterAvailable sinceMay 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTRIP-CMV-GFP-FLAG-cGAS E225A-D227A
Plasmid#86674PurposeExpresses catalytically-inactive GFP-cGAS after lentiviral transduction. Can be used to monitor nuclear envelope rupture events.DepositorInsertGFP-FLAG-cGAS E225A/D227A
UseLentiviralTagsExpressionMutationPromoterAvailable sinceApril 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGP-AAV-syn-FLEX-jGCaMP8s-WPRE
Plasmid#162377PurposeAAV-mediated expression of ultrafast protein calcium sensor under the Syn promoter, Cre-dependent expressionDepositorHas ServiceAAV Retrograde, AAV1, and AAV9InsertjGCaMP8s
UseAAV and Cre/LoxTags6xHisExpressionMammalianMutationS26M F286Y Q315HPromoterSynapsinAvailable sinceNov. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFlagCMV2-UbcH8
Plasmid#12442DepositorInsertUbcH8 (UBE2L6 Human)
UseTagsFLAGExpressionMammalianMutationPromoterAvailable sinceJan. 4, 2007AvailabilityAcademic Institutions and Nonprofits only -
pSDMA57
Plasmid#67941PurposeComplementation in Cryptococcus neoformans. Targets DNA constructs to a Safe Haven Site, a small gene-free region. Contains NEO resistance marker.DepositorTypeEmpty backboneUseCryptococcal complementation vectorTagsExpressionMutationPromoterACT1 for Cryptococcal markerAvailable sinceOct. 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLentiPGK Blast DEST JNKKTRmRuby2
Plasmid#59154PurposeLentiviral vector to express JNK KTR mRuby2 under PGK promoter (With Blasticidin Resistance)DepositorInsertJNK Kinase Translocation Reporter (MAPK8 Human)
UseLentiviralTagsmRuby2ExpressionMammalianMutationPromoterPGKAvailable sinceSept. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pATetO 6XHis-Taq DNA Pol
Plasmid#178782PurposeInducible expression of Taq DNA polymerase in E. coliDepositorInsertTaq DNA Polymerase
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
Arl6ip1-mEmerald
Plasmid#186965PurposeExpress Arl6ip1 with C-terminus mEmeraldDepositorInsertARL6IP1 (ARL6IP1 Human)
UseTagsmEmeraldExpressionMammalianMutationPromoterCMV PromoterAvailable sinceSept. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPR.v2.TetON_Cas13d_U6_DR
Plasmid#196727PurposeSortable and selectable, Tet-inducible RfxCas13d with guide cassette (one vector system). Inducible knock-down.DepositorInsertRfxCas13d-T2A-miRFP670nano
UseCRISPR and LentiviralTagsNucleoplasmin NLS and SV40 NLSExpressionMutationPromoterTREAvailable sinceApril 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
mCherry-NES-SspB-MCS-P2A-iLIDcaax
Plasmid#173869PurposeMammalian expression of mCherry-NES-SspB-P2A-iLIDcaax (opto-control) containing multiple cloning sites (MCS)DepositorInsertmCherry-NES-SspB-MCS-P2A-iLIDcaax
UseTagsC-terminal CAAX-box and mCherryExpressionMammalianMutationPromoterAvailable sinceSept. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti-Lifeact-tdTomato
Plasmid#64048PurposeFluorescent reporter for F-actin labeling in living cellsDepositorInsertLifeact
UseLentiviralTagstdTomatoExpressionMammalianMutationPromoterUbiquitinAvailable sinceJuly 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
mCherry-Sec23A
Plasmid#166894Purposemammalian expression of Sec23A tagged with mCherryDepositorInsertSEC23A (SEC23A Human)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceApril 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-GUCY2C
Plasmid#116749PurposeLentiviral expression of GUCY2CDepositorInsertGUCY2C (GUCY2C Human)
UseLentiviralTagsExpressionMutationWTPromoterAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-hHSF1 WT
Plasmid#71724Purposeplasmid expressing myc-his tag hHSF1 WTDepositorInsertHSF1 (HSF1 Human)
UseTagsMYC 6xHISExpressionMammalianMutationPromoterAvailable sinceMay 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
Human pIgR(SC)
Plasmid#158216PurposeExpress the ectdomain (SC) of human pIgRDepositorInsertPIGR (PIGR Human)
UseTags8×hisExpressionMammalianMutationPromoterAvailable sinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
TFORF1768
Plasmid#143541PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertZNF28 (ZNF28 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJuly 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBBK21 pCAS-Tyr-[gRNA: 4=XII-5] (SplitHygR, AmpR)
Plasmid#179005PurposeSp.Cas9 and gRNA yeast expression vector withXII-5 gRNA pre-cloned. Selection =SplitHygromycin resistanceDepositorInsertS. pyogenes Cas9
UseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceMay 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBBK20 pCAS-Tyr-[gRNA: 3=XII-2] (SplitHygR, AmpR)
Plasmid#179004PurposeSp.Cas9 and gRNA yeast expression vector with XII-2 gRNA pre-cloned. Selection =SplitHygromycin resistanceDepositorInsertS. pyogenes Cas9
UseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceMay 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
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mKeima-Red-N1
Plasmid#54597PurposeLocalization: N1 Cloning Vector, Excitation: 440, Emission: 620DepositorTypeEmpty backboneUseTagsmKeima-RedExpressionMammalianMutationPromoterAvailable sinceJune 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
FRP791_insul-(lexA-box)1-PminCYC1-Citrine-TCYC1
Plasmid#58432PurposeInduces Citrine expression from a promoter containing 1 lexA box in yeast cellsDepositorInsertCitrineA206K
UseTagsExpressionYeastMutationPromoterinsul-(lexA-box)1-PminCYC1Available sinceAug. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hsyn-GRAB_DA2h
Plasmid#140554PurposeExpresses the 2nd generation high-affinity DA sensor GRAB_DA2h in neuronsDepositorHas ServiceAAV9InsertThe 2nd generation DA sensor GRAB_DA2h (high affinity)
UseAAVTagsExpressionMutationPromoterhSynAvailable sinceJuly 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pADSL-EGFP
Plasmid#99109PurposeExpresses human ADSL-EGFP fusion in mammalian cellsDepositorInsertADSL (ADSL Human)
UseTagsEGFP (enhanced green fluorescent protein)ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLX302 EGFP-V5
Plasmid#141348PurposeExpresses V5 tagged GFPDepositorInsertEGFP
UseLentiviralTagsV5 tagExpressionMammalianMutationPromoterAvailable sinceJuly 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLVUHshp53
Plasmid#11653DepositorInsertGFP, shRNA against TP53 (TP53 Human)
UseCre/Lox, Lentiviral, and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceAug. 17, 2006AvailabilityAcademic Institutions and Nonprofits only -
pCMV-PE4stem
Plasmid#208768PurposePE2max that co-express dnMLH1 and p53DDDepositorInsertPE4max with 2A-p53DD
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV.CMV.HI.eGFP-Cre.WPRE.SV40
Plasmid#105545PurposeAAV expression of EGFP-Cre from CMV promoterDepositorHas ServiceAAV1, AAV2, AAV5, AAV8, and AAV9InsertEGFP-Cre
UseAAV and Cre/LoxTagsExpressionMammalianMutationPromoterHIAvailable sinceFeb. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET His6 MBP prescission LIC cloning vector (HMPKS)
Plasmid#29721DepositorTypeEmpty backboneUseTagsHis6-MBP-prescissionExpressionBacterialMutationPromoterAvailable sinceSept. 17, 2013AvailabilityAcademic Institutions and Nonprofits only -
pSCMV-H2B-FT-Medium
Plasmid#157670PurposeRetroviral vector for expressing chimeric human histone H2B fused with the "Medium" variant of the monomeric color-changing fluorescent timer (FT) in mammalian cells.DepositorInsertHistone H2B
UseRetroviralTagsMedium-FTExpressionMammalianMutationPromoterCMVAvailable sinceAug. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
hSLBP_mNG_FS_Donor
Plasmid#191387PurposeDonor for C-terminal tagging of endogenous hSLBPDepositorInsertmNeonGreen(mNG) + FS(3xFLAG_TwinStrep)Tag for hSLBP tagging at the C-terminus (SLBP Human, Synthetic)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBR322-PVCpnf17-22
Plasmid#198272PurposeUnmodified pPayload; contains WT PVC payloads/regulatory genesDepositorInsertPVCpnf17-22
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRSET his-mCherry
Plasmid#113552PurposeFor bacterial expression and purification of mCherryDepositorInserthis-mCherry
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pIB184 (EM)
Plasmid#90194PurposeE. coli - Streptococci shuttle plasmid for gene expression in streptococci with P23 promoterDepositorTypeEmpty backboneUseTagsNoneExpressionBacterialMutationPromoterAvailable sinceApril 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTRANS_201
Plasmid#91105PurposeTransformation, Type: T-DNA, Plant Selection: none, Viral Replication: BeYDVDepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterNo PromoterAvailable sinceJuly 14, 2017AvailabilityAcademic Institutions and Nonprofits only