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Showing: 4281 - 4300 of 5448 results

  1. pLCHKO_HPRT1_exon3_1_Lb

    Plasmid
    #155053
    Purpose
    Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of HPRT1 exon 3 using SpCas9 and LbCas12a nucleases (CHyMErA system)
    Depositor
    Jason Moffat, Benjamin Blencowe
    Article
    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.
    Insert
    (hg)RNA for deletion of HPRT1 exon 3
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    Aug. 12, 2020
    Availability
    Academic Institutions and Nonprofits only
    155053-plasmid-map-sequence-id-308427

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  6. KG#84

    Plasmid
    #110878
    Purpose
    Expresses the C. elegans acy--1 P260S gain-of-function cDNA in ventral cord cholinergic motor neurons
    Depositor
    Kenneth Miller
    Article
    Schade et al Genetics. 2005 Feb;169(2):631-49. doi: 1
    Inserts
    unc-17beta promoter
    acy-1 P260S gain-of-function cDNA
    unc-54 3' control region with 1 artificial intron just upstream and 1 artificial intron in control region
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Changed Proline to Serine at amino acid 260
    Promoter
    Available since
    Aug. 8, 2018
    Availability
    Academic Institutions and Nonprofits only
    110878-plasmid-map-sequence-id-217951

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  7. KG#81

    Plasmid
    #110876
    Purpose
    Expresses the C. elegans acy-1 P260S gain-of-function cDNA in body wall muscle
    Depositor
    Kenneth Miller
    Article
    Schade et al Genetics. 2005 Feb;169(2):631-49. doi: 1
    Inserts
    myo-3 promoter
    acy-1 P260S gain-of-function cDNA
    unc-54 3' control region with 1 artificial intron just upstream and 1 artificial intron in control region
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Changed Proline to Serine at amino acid 260
    Promoter
    Available since
    June 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    110876-plasmid-map-sequence-id-212571

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  8. KG#83

    Plasmid
    #110877
    Purpose
    Expresses the C. elegans acy--1 P260S gain-of-function cDNA pan-neuronally
    Depositor
    Kenneth Miller
    Article
    Schade et al Genetics. 2005 Feb;169(2):631-49. doi: 1
    Inserts
    rab-3 promoter
    acy-1 P260S gain-of-function cDNA
    unc-54 3' control region with 1 artificial intron just upstream and 1 artificial intron in control region
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Changed Proline to Serine at amino acid 260
    Promoter
    Available since
    June 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    110877-plasmid-map-sequence-id-212517

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  14. AICSDP-23:TJP1-mEGFP

    Plasmid
    #87429
    Purpose
    Homology arms and linker-mEGFP sequence for N-terminus tagging of human TJP1
    Depositor
    Allen Institute for Cell Science
    Article
    Roberts et al Mol Biol Cell. 2017 Oct 15;28(21):2854-2
    Insert
    TJP1 Homology Arms with mEGFP-linker (TJP1 Human)
    Use
    CRISPR; Donor template
    Tags
    mEGFP-linker
    Expression
    Mammalian
    Mutation
    homology arms contain point mutations to disrupt …
    Promoter
    Available since
    March 21, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
    87429-plasmid-map-sequence-id-174170

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  18. TFORF0392

    Plasmid
    #142258
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    ZNF7 (ZNF7 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    June 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    142258-plasmid-map-sequence-id-395750

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  20. pKK-BiFC-Venus

    Plasmid
    #105804
    Purpose
    Concomitant expression of two proteins in fusion with fragments of Venus protein. Protein interactions studies by bimolecular fluorescence complementation (BiFC), including BiFC-FRET assays.
    Depositor
    Andrzej Dziembowski
    Article
    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do
    Type
    Empty backbone
    Use
    Flp-in competent
    Tags
    VN173 (I152L); VC155
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 12, 2018
    Availability
    Academic Institutions and Nonprofits only
    105804-plasmid-map-sequence-id-203400

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Showing: 4281 - 4300 of 5448 results