-
Plasmid#101245PurposeGenetically-encoded calcium actuator; mCherry-tagged LOV2-STIM1 chimera that enables optical control of calcium signals and calcium dependent signalingDepositorInsertLOV2 and STIM1 cytosolic fragment
UseTagsmCherryExpressionBacterial and MammalianMutationPromoterCMVAvailable sinceApril 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
PKD1-F (pCI-PKD1-Flag)
Plasmid#21369DepositorInsertautosomal dominant polycystic kidney disease type I (PKD1 Human)
UseTagsFLAGExpressionMammalianMutationwild-type sequence*PromoterAvailable sinceJan. 22, 2010AvailabilityAcademic Institutions and Nonprofits only -
FPR1-Tango
Plasmid#66283PurposeExpression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocationDepositorInsertFPR1 (FPR1 Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCDF-MCM4/6
Plasmid#116951PurposeExpresses human MCM4 and human MCM6DepositorUseTagsHis6ExpressionBacterialMutationPromoterAvailable sinceNov. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV-PE2-NG
Plasmid#159977PurposePrime editing in mammalian cellsDepositorInsertPE2-NG
UseTagsBpSV40 NLSExpressionMammalianMutationPromoterCMVAvailable sinceNov. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
MIGR1
Plasmid#27490DepositorTypeEmpty backboneUseRetroviralTagsGFP and IRESExpressionMammalianMutationPromoterAvailable sinceMarch 18, 2011AvailabilityAcademic Institutions and Nonprofits only -
pSL0527 (pDonor)
Plasmid#130634PurposeEncodes a mini-transposon derived from V. cholerae CAST system, with CmR cargo gene. Total transposon size = 977 bp.DepositorInsertVchCAST donor DNA (Mini-Tn)
UseCRISPR; TransposonTagsExpressionBacterialMutationPromoterAvailable sinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMOD_A0101
Plasmid#90998PurposeModule A, Promoter: 35S, Gene: AtCas9, Terminator: HSPDepositorInsertAtCas9
UseCRISPRTagsExpressionMutationPromoter35SAvailable sinceJuly 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLV CMV mCherry-DDX21-V5
Plasmid#175163PurposeLentiviral expression of human DDX21-V5DepositorInsertDDX21 (DDX21 Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterAvailable sinceJan. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHAGE2_EF1a_TetOn_IRES_mCherry
Plasmid#204155PurposeLentivirus backbone expressing rtTA linked to mCherryDepositorInsertTet-On 3G
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceAug. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
mApple-TfR-RUSH
Plasmid#166906PurposeRUSH cargo: Str-KDEL_IRES_Transferrin Receptor (TfR)-SBP-mAppleDepositorInsertmApple
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
SunTagng22aa
Plasmid#106438PurposeEncodes a SunTag CRISPR cas9 system that does not contain a guide RNA and therefore, does not target the TET1 catalytic domain to any specific region of the genome.DepositorInsertNOS_TET1CD_2xNLS_1xHA__sfGFP_scFv_UBQ10_INSULATOR_UBQ10_dCAS9_1xHA_3xNLS_10xGCN422aa_OCS
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceMarch 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pmyc-GFP-TNRC6A
Plasmid#41999DepositorInsertTNRC6A (TNRC6A Human)
UseTagsEGFP and MycExpressionMammalianMutationPromoterCMVAvailable sinceMarch 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-Puro CAG CLTX-CAR
Plasmid#157743Purposeknock CLTX-CAR into AAVS1 safe harbor locus for constitutive expressionDepositorInsertchlorotoxin containing chimeric antigen receptor targeting glioblastoma
UseCRISPR, Synthetic Biology, and TALENTagsExpressionMammalianMutationPromoterCAGAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJL1
Plasmid#69496PurposepJL1 expression vector with sfGFPDepositorInsertSuper folder GFP
UseTagsExpressionBacterialMutationPromoterT7Available sinceOct. 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAG504-frFAST
Plasmid#160055PurposeExpresses frFAST in mammalian cellsDepositorInsertfrFAST
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
piRFP720-N1
Plasmid#45461DepositorInsertiRFP720
UseTagsExpressionMammalianMutationT2A, S13L, A92T, V104T, V114I, E161K, E180S, Y193…PromoterCMVAvailable sinceJune 17, 2013AvailabilityAcademic Institutions and Nonprofits only -
pMSP3545
Plasmid#46888PurposeGram-Positive Bacterial Shuttle Vector for Nisin-Controlled Inducible ExpressionDepositorTypeEmpty backboneUseNisin-inducible expression; gram-positive bacteri…TagsExpressionBacterialMutationPromoterPnisAAvailable sinceSept. 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCAGIG
Plasmid#11159PurposeMammalian expression vector for cloning and expressing your gene on a bicistronic mRNA also encoding IRES-GFPDepositorHas ServiceCloning Grade DNAInsertIRES-EGFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 13, 2006AvailabilityAcademic Institutions and Nonprofits only -
pShuttle
Plasmid#16402PurposeShuttle vector for us in AdEasy System. Used for expression of transgenes under a chosen (non-CMV) promoter when no GFP tracer is desired.DepositorTypeEmpty backboneUseAdenoviralTagsExpressionMammalianMutationPromoterAvailable sinceJune 11, 2008AvailabilityAcademic Institutions and Nonprofits only -
pMT416
Plasmid#8607DepositorInsertbarnase, barstar
UseTagsphoA signal sequence to barnaseExpressionBacterialMutationPromoterAvailable sinceJan. 17, 2007AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO V5
Plasmid#19445DepositorInsertV5
UseTagsExpressionMammalianMutationV5 tag inserted to create new vectorPromoterAvailable sinceOct. 6, 2008AvailabilityAcademic Institutions and Nonprofits only -
Nrxn3b-Nav1.6-MKII-hLOV1-TEVcs(ENLYFQ/M)-tTA-VP16
Plasmid#163029PurposeFLiCRE TF for neuronal expressionDepositorInsertNrxn3b-MKII-hLOV1-TEVcs-tTA
UseTagsExpressionMammalianMutationPromoterSynapsinAvailable sinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGIPz-mPD-L1
Plasmid#121488PurposeExpression of mouse PD-L1 WTDepositorInsertPD-L1
UseLentiviralTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGP-20XUAS-IVS-Syn21-RSET-jGCaMP8m-p10
Plasmid#218862PurposeGal4 activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogasterDepositorInsertRSET-jGCaMP8m
UseTags6xHis-T7 gene 10 leader-Xpress epitope-Enterokina…ExpressionInsectMutationA25G F286YPromoterhsp70Available sinceMay 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMVHA EED wt
Plasmid#24231PurposeMammalian expression of human EED with HA tagDepositorInsertEED (EED Human)
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceJan. 25, 2011AvailabilityAcademic Institutions and Nonprofits only -
Syn-ATP
Plasmid#51819PurposeOptical reporter of presynaptic ATPDepositorInsertChimeric Synaptophysin-mCherry-Luciferase
UseTagsExpressionMammalianMutationWithin WT luciferase gene, changed Threonine 214 …PromoterCMVAvailable sinceMarch 13, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBAM1
Plasmid#60487PurposeThis plasmids is a modular mini-Tn5 vector that could be used to generate random mutagenesis libraries or to insert heterologous DNA into a target genomeDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceOct. 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pNOC-CRISPR-GFP
Plasmid#100009PurposeExpresses Cas9-GFP and sgRNA in Nannochloropsis oceanica, contains hygromycin resistance cassette, for integration into geneomeDepositorTypeEmpty backboneUseAlgae, nannochloropsis expressionTagsCas9-GFPExpressionMutationPromoterRibi promoterAvailable sinceFeb. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
mTagRFP-T-Lifeact-7
Plasmid#54586PurposeLocalization: Actin, Excitation: 555, Emission: 584DepositorTypeEmpty backboneUseTagsLifeAct -mTagRFP-TExpressionMammalianMutationPromoterAvailable sinceJuly 25, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pC019 - LwCas13a from Leptotrichia wadei
Plasmid#91909PurposeExpresses LwCas13a for bacterial expression and contains backbone for spacer cloningDepositorInsertLwCas13a and guide expression cassette
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceOct. 4, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
V96 ELP
Plasmid#68392PurposeElastin-like polypeptide (VPGxG) with 96 repeats of Valine guest residueDepositorInsertV96 ELP
UseTagsExpressionBacterialMutationPromoterT7Available sinceOct. 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pICE-FLAG-NAT10-siR-WT
Plasmid#59365PurposePlasmid for constitutive or doxycycline-inducible expression of wild-type human NAT10 resistant to a siRNA. Confers resistance to puromycin. Use T-REx cells for doxycycline-inducible expression.DepositorInserthuman NAT10 (NAT10 Human)
UseTagsFLAGExpressionMammalianMutationMutations to render cDNA resistant to a siRNAPromoterCMV-tetAvailable sinceOct. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAM-CAGGS-FLEX-H2B-3XHA-P2A-N2c(G)
Plasmid#73477PurposeEncodes the G protein to enable transsynaptic RAV infectionDepositorInsertH2B-3XHA-P2A-N2c(G)
UseAAVTagsExpressionMutationPromoterAvailable sinceMarch 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-Mito-HyPerGRt
Plasmid#173198PurposeAAV transfer plasmid for a HyPer7 and tdTomato fusion under hSyn promoterDepositorInsertHyper7-tdTomato
UseAAVTagsExpressionMutationPromoterhSynAvailable sinceMarch 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
PB-fLuc=tdT
Plasmid#120870PurposePiggybac transposon plasmid for live animal optical imagingDepositorInsertpcDNA3-fLuc=tdT
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceFeb. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
S1PR3-Tango
Plasmid#66498PurposeExpression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocationDepositorInsertS1PR3 (S1PR3 Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
iMbody_pSANG10-3F
Plasmid#184496PurposeExpresses iMbody-6xHis-3xFLAG in BL21(DE3) bacteriaDepositorInsertiMbody
UseTags3xFLAG, 6xHis, and pelB leaderExpressionBacterialMutationPromoterAvailable sinceNov. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-EGFP-Cdc42-T17N
Plasmid#12976DepositorInsertCdc42 DN (CDC42 Human)
UseTagsEGFPExpressionMammalianMutationT17N Dominant negativePromoterCMVAvailable sinceOct. 27, 2006AvailabilityAcademic Institutions and Nonprofits only -
PKA-KTR-mStrawberry reporter
Plasmid#158688PurposeThis plasmid is a PKA pathway reporter. It has CMV promoter driving the expression of PKA-KTR fused to mStrawberry-pGK-BSDDepositorInsertmStrawberry
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
MSCV-neo C-HAonly ZER1
Plasmid#191540PurposeProtein expression of ZER1DepositorInsertZER1 (ZER1 Human)
UseRetroviralTagsHAExpressionMutationPromoterAvailable sinceNov. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRRL_SRSF2_P95H_mCherry
Plasmid#84023Purposeexpression of SRSF2 mutant in mammalian cellsDepositorInsertSRSF2 (SRSF2 Human)
UseLentiviralTags2TA-mCherry and FlagExpressionMammalianMutationP95HPromoterPGKAvailable sinceOct. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSGNluc
Plasmid#186705PurposeA high-sensitivity bi-directional reporter to monitor NF-κB activity in cell culture and zebrafish in real timeDepositorInsertsGFP
luciferase
UseTagsExpressionMutationPromoterAvailable sinceSept. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCG_SARS-CoV1 NSP3 C-V5-tag
Plasmid#179962PurposeMammalian expression vector for SARS-CoV-1 Nsp3, V5-tagged. Sequence codon-optimized.DepositorInsertSARS-CoV-1 Nsp3 (ORF1ab Synthetic)
UseTagsExpressionMammalianMutationhuman codon-optimizedPromoterAvailable sinceFeb. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMXs-hs-EGR1
Plasmid#52724Purposeretroviral expression of human EGR1DepositorInsertEGR1 (EGR1 Human)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceJune 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEF-BFP
Plasmid#138272PurposeExpression of engineered BFP variant that undergoes conversion to GFP after targeted modification with a cytidine deaminase-based DNA base editorDepositorInsertBFP H66 PAM
UseCRISPRTagsExpressionMammalianMutationH66, PAM motifPromoterEf1aAvailable sinceMarch 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
hprtSAGFP
Plasmid#41594PurposeIn vivo single-strand annealing substrate composed of two truncated GFP genes, separated by a drug selection marker, and flanked by mouse hprt targeting sequencesDepositorInsertSA-GFP reporter
UseMouse Targeting; Dsb repair reporter substrateTagsExpressionMammalianMutationPromoterAvailable sinceDec. 3, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Neo-Bam p53 R248W
Plasmid#16437DepositorInsertp53 R248W (TP53 Human)
UseTagsExpressionMammalianMutationR248WPromoterAvailable sinceAug. 4, 2008AvailabilityAcademic Institutions and Nonprofits only -
pLVX-EF1alpha-SARS-CoV-2-nsp13-2xStrep-IRES-Puro
Plasmid#141379PurposeLentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirusDepositorInsertSARS-CoV-2-nsp13 (ORF1ab SARS-CoV-2)
UseLentiviralTags2xStrepExpressionMammalianMutationhuman codon optimizedPromoterEF1alphaAvailable sinceApril 9, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCFJ90 - Pmyo-2::mCherry::unc-54utr
Plasmid#19327DepositorArticleInsertPmyo-2::mCherry
UseTagsExpressionWormMutationPromoterAvailable sinceOct. 15, 2008AvailabilityAcademic Institutions and Nonprofits only