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Plasmid#63024PurposeTo express Gal4 in the pattern of the Drosophila melanogaster odorant receptor Or22c, using the promoter sequences of the gene cloned 5' of Gal4DepositorInsertDrosophila melanogaster Or22c promoter
UseTagsDrosophila ADH (aa's 1-18) fused to Yeast Ga…ExpressionInsectMutationPromoterAvailable sinceDec. 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
Or24a-Gal4
Plasmid#63026PurposeTo express Gal4 in the pattern of the Drosophila melanogaster odorant receptor Or24a, using the promoter sequences of the gene cloned 5' of Gal4DepositorInsertDrosophila melanogaster Or24a promoter
UseTagsDrosophila ADH (aa's 1-18) fused to Yeast Ga…ExpressionInsectMutationPromoterAvailable sinceNov. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDGB3_omega1R
Plasmid#68240PurposeGoldenBraid level 1 Omega plant expression vectorDepositorInsertempty vector
UseSynthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceOct. 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLKO1-puro-U6-sgRNA-TS2
Plasmid#69234PurposeU6 driven SpCas9 sgRNA expression for TS2 siteDepositorInsertTS2 sgRNA
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceOct. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLKO1-puro-U6-sgRNA-TS3
Plasmid#69235PurposeU6 driven SpCas9 sgRNA expression for TS3 siteDepositorInsertTS3 sgRNA
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceOct. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLKO1-puro-U6-sgRNA-TS4
Plasmid#69236PurposeU6 driven SpCas9 sgRNA expression for TS4 siteDepositorInsertTS4 sgRNA
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceOct. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDGB3_omega2R
Plasmid#68241PurposeGoldenBraid level 1 Omega plant expression vectorDepositorInsertempty vector
UseSynthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceOct. 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
Or83a-Gal4
Plasmid#63050PurposeTo express Gal4 in the pattern of the Drosophila melanogaster odorant receptor Or83a, using the promoter sequences of the gene cloned 5' of Gal4DepositorInsertDrosophila melanogaster Or83a promoter
UseTagsDrosophila ADH (aa's 1-18) fused to Yeast Ga…ExpressionInsectMutationPromoterAvailable sinceOct. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pATJ (pKD-G8)
Plasmid#62687PurposeMammalian expression of amiR-eGFP from the broadly active CAG promoter/enhancerDepositorInsertamiR-eGFP419/amiR-eGFP123
UseRNAiTagsExpressionMammalianMutationPromoterAvailable sinceAug. 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
Or49a-Gal4
Plasmid#63039PurposeTo express Gal4 in the pattern of the Drosophila melanogaster odorant receptor Or49a, using the promoter sequences of the gene cloned 5' of Gal4DepositorInsertDrosophila melanogaster Or49a promoter
UseTagsDrosophila ADH (aa's 1-18) fused to Yeast Ga…ExpressionInsectMutationPromoterAvailable sinceApril 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
Or63a-Gal4
Plasmid#63043PurposeTo express Gal4 in the pattern of the Drosophila melanogaster odorant receptor Or63a, using the promoter sequences of the gene cloned 5' of Gal4DepositorInsertDrosophila melanogaster Or63a promoter
UseTagsDrosophila ADH (aa's 1-18) fused to Yeast Ga…ExpressionInsectMutationPromoterAvailable sinceApril 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
hs-leonardo
Plasmid#42065DepositorInsertleonardo (14-3-3zeta Fly)
UseTagsExpressionInsectMutationPromoterHsp70Available sinceFeb. 20, 2013AvailabilityAcademic Institutions and Nonprofits only -
spitz (9 kb)
Plasmid#38340DepositorInsertspitz (spi Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceSept. 12, 2012AvailabilityAcademic Institutions and Nonprofits only -
pJRH029
Plasmid#171060PurposePlasmid for expressing Gag fused to spyCas9-3x FLAG-2x SV40 NLS with the SQNYPIVQ HIV-1 cleavage site in betweenDepositorInsertGag-spyCas9
UseCRISPR and LentiviralTags2x SV40 NLS and 3x FLAG tagExpressionMammalianMutationPromoterCAGAvailable sinceJune 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
Lenti-iCas9-neo
Plasmid#85400PurposeLentiviral vector encoding a doxycycline inducible EGFP reporter downstream of FLAG-tagged spCas9, separated by a P2A self-cleavage sequenceDepositorInsertFlag-iCas9-P2A-GFP
UseLentiviralTagsFlag-iCas9-P2A-GFPExpressionMutationPromoterAvailable sinceJan. 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
ABE8e(TadA-8e V106W)
Plasmid#138495PurposeA-to-G base editorDepositorInsertecTadA(8e V106W)-nSpCas9
UseTagsExpressionMammalianMutationTadA-8e V106WPromoterAvailable sinceMarch 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJRH-1179 U6-reci Gag-Cas9 v2
Plasmid#201914PurposeCas9-EDV production plasmid. Expresses the v2 Gag-Cas9 polypeptide (CAG promoter) and sgRNA (human U6 promoter. BsmBI cutsites for spacer insertion)DepositorInsertGag-Cas9 v2
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceAug. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
CMPE-ePPEplus
Plasmid#205242PurposeFor multiplex plant prime editing in wheat plants or monocotyledons protoplastsDepositorInsertCsy4-c-myc NLS-bpNLS-nCas9-NC-MLV-vbpNLS-SV40NLS
UseCRISPRTagsExpressionPlantMutationDetailed in manuscriptPromoterAvailable sinceOct. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
A3A-PBE
Plasmid#119768PurposeConversion of C to T in wheat plants or monocotyledons protoplastsDepositorInsertAPOBEC3A-nCas9-NLS-UGI-NLS
UseTagsNLS (flanking UGI)ExpressionPlantMutationnCas9 has a mutation D10A and E945K in wild type …Promotermaize Ubiquitin-1Available sinceJune 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pX330-UGCG-KO
Plasmid#80010PurposegRNA to knock out expression of UGCG gene. The product of this gene catalyzes the first step in synthesis of glycosphingolipids.DepositorInsertUGCG (UGCG Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJuly 25, 2016AvailabilityAcademic Institutions and Nonprofits only