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Plasmid#117555PurposeLevel1 Golden Gate Cassette: sgRNA cassette for spCas9, targeting NbPDS gene in N. benthamiana with 1 mismatch at spacerDepositorInsertPromoter_AtU6-26 + sgRNA_NbPDS1 _1mismatch N. benthamiana
UseTagsExpressionPlantMutationPromoterAtU6-26 (pICSL90002)Available sinceNov. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEPOR1CB0018
Plasmid#117554PurposeLevel1 Golden Gate Cassette: sgRNA cassette for spCas9, targeting NbPDS gene in N. benthamiana with 1 mismatch at spacerDepositorInsertPromoter_AtU6-26 + sgRNA_NbPDS1 _1mismatch N. benthamiana
UseTagsExpressionPlantMutationPromoterAtU6-26 (pICSL90002)Available sinceNov. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEPOR1CB0022
Plasmid#117537PurposeLevel1 Golden Gate Cassette: estended stem sgRNA cassette for SpCas9 targeting NbPDS gene in N. benthamianaDepositorInsertsgRNA (Sp Cas9) - extended stem
UseTagsExpressionPlantMutationPromoterAvailable sinceNov. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pQE80L TriCatcher-MMP
Plasmid#112632PurposeTwo SpyCatchers linked by an elastin-like polypeptide with an MMP-cleavable sequence and a C-terminal SnoopCatcherDepositorInsertTriCatcher-MMP
UseTagsHis6 and TEV cleavage siteExpressionBacterialMutationcentral matrix metalloproteinase-cleavable sequen…PromoterT5Available sinceSept. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
gnu-GFP 9A
Plasmid#113006PurposeP element vector for transposon insertion of gnu-GFP 9A into Drosophila genomeDepositorInsertfull length gnu (gnu Fly)
UseP insertion vectorTagsGFPExpressionMutation9 CycB/CDK1 sites mutated to APromoterAvailable sinceAug. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
gnu-GFP WT
Plasmid#113005PurposeP element vector for transposon insertion of gnu-GFP WT into Drosophila genomeDepositorInsertfull length gnu (gnu Fly)
UseP insertion vectorTagsGFPExpressionMutationPromoterAvailable sinceAug. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBW1944_ hU6-PV4-loxP-BbsI-ENTRY-gRNA
Plasmid#87572PurposePV4 gRNA entry vector for CRISPR decoderDepositorTypeEmpty backboneUseCre/Lox and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceMay 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBW1943_ hU6-PV3-FRT-loxP-BbsI-ENTRY-gRNA
Plasmid#89056PurposePV3 gRNA entry vector for CRISPR decoderDepositorTypeEmpty backboneUseCre/Lox and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceApril 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
mCm7RNA6A
Plasmid#84367PurposeCas9-gRNA plasmid for mouse Cbfa2t2 m7 mutant knockinDepositorInsertCbfa2t2 m7-gRNA6
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceFeb. 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHU6_Ch5_155183064-gRNA-rev
Plasmid#81215PurposepHu6-gRNA-NT1_SpecR backbone, gRNA for targeting the 5' region of pCALNL_Ch5 reporterDepositorInserthU6 expression of gRNA
UseTagsExpressionMammalianMutationPromoterU6Available sinceNov. 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
CAG-mCherry-RORbshRNA2
Plasmid#73984PurposeRORb shRNA2 (mir155 backbone) was inserted into the 3'UTR of mCherry.DepositorInsertshRNA that targets the mouse RORb gene
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceMay 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
CAG-EGFP-RORbshRNA1
Plasmid#73985PurposeRORb shRNA1 (mir155 backbone) was inserted into the 3'UTR of EGFP.DepositorInsertshRNA that targets the mouse RORb gene
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceMay 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
CAG-EGFP-RORbshRNA2
Plasmid#73986PurposeRORb shRNA2 (mir155 backbone) was inserted into the 3'UTR of EGFP.DepositorInsertshRNA that targets the mouse RORb gene
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceMay 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
CAG-mCherry-Otx2shRNA2
Plasmid#73980PurposeOtx2 shRNA2 (mir155 backbone) was inserted into the 3'UTR of mCherry.DepositorInsertshRNA that targets the mouse Otx2 gene
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceMay 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
Gg 3kb Green opsin GFP
Plasmid#72919Purposegreen opsin promoter from chicken (Gallus gallus) gDNA chr26:4,504,913–4,501,931 in galGal4 driving GFPDepositorInsertchicken green opsin promoter
UseTagsExpressionMutationPromoterAvailable sinceFeb. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
Gg 3kb Green opsin DsRed
Plasmid#72918Purposegreen opsin promoter from chicken (Gallus gallus) gDNA chr26:4,504,913–4,501,931 in galGal4 driving DsRedDepositorInsertchicken green opsin promoter
UseTagsExpressionMutationPromoterAvailable sinceFeb. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJB051 (mEos2-ZapA)
Plasmid#72650PurposeInducible expression of mEos2-ZapA in bacteriaDepositorInsertmEos2-ZapA
UseTagsmEos2ExpressionBacterialMutationPromoterT5-lacAvailable sinceFeb. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
Or59a-Gal4
Plasmid#63042PurposeTo express Gal4 in the pattern of the Drosophila melanogaster odorant receptor Or59a, using the promoter sequences of the gene cloned 5' of Gal4DepositorInsertDrosophila melanogaster Or59a promoter
UseTagsDrosophila ADH (aa's 1-18) fused to Yeast Ga…ExpressionInsectMutationPromoterAvailable sinceDec. 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
Or22c-Gal4
Plasmid#63024PurposeTo express Gal4 in the pattern of the Drosophila melanogaster odorant receptor Or22c, using the promoter sequences of the gene cloned 5' of Gal4DepositorInsertDrosophila melanogaster Or22c promoter
UseTagsDrosophila ADH (aa's 1-18) fused to Yeast Ga…ExpressionInsectMutationPromoterAvailable sinceDec. 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
Or24a-Gal4
Plasmid#63026PurposeTo express Gal4 in the pattern of the Drosophila melanogaster odorant receptor Or24a, using the promoter sequences of the gene cloned 5' of Gal4DepositorInsertDrosophila melanogaster Or24a promoter
UseTagsDrosophila ADH (aa's 1-18) fused to Yeast Ga…ExpressionInsectMutationPromoterAvailable sinceNov. 17, 2015AvailabilityAcademic Institutions and Nonprofits only