Addgene

We narrowed to 27,415 results for: eng

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p3PA-Puro-iTk

Plasmid

#109067

Purpose

inserts three polyA sites to end transcription of a targetted gene and a puromycin cassette for selection

Depositor

Eric Lander

Article

Engreitz et al Nature. 2016 Nov 17;539(7629):452-455. d

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

EFS

Available since

Sept. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pTK_Msx1_enh_264_Citrine

Plasmid

#130610

Purpose

enhancer reporter

Depositor

Tatjana Sauka-Spengler

Article

Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

Insert

Msx1_enh_264

Use

Enhancer reporter

Tags

Expression

Mutation

Promoter

thymidine kinase minimal promoter

Available since

Oct. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pILGFP4I

Plasmid

#104504

Purpose

Used to evaluate the expression output of Saccharomyces kudriavzevii GAL1 promoter with yEGFP used as the reporter gene

Depositor

Claudia Vickers

Article

Peng et al ACS Synth Biol. 2018 Feb 16;7(2):748-751

Insert

SkGAL1 promoter-yEGFP

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Dec. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pILGFP4Q

Plasmid

#104512

Purpose

Used to evaluate the expression output of Saccharomyces kudriavzevii GAL2 promoter with yEGFP used as the reporter gene

Depositor

Claudia Vickers

Article

Peng et al ACS Synth Biol. 2018 Feb 16;7(2):748-751

Insert

SkGAL2 promoter-yEGFP

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Dec. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pLY108

Plasmid

#130938

Purpose

Engineered sgRNA-LEB1 (2 BoxB aptamers) generator circuit including promoter Plux2

Depositor

Baojun Wang

Article

Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:

Insert

sgRNA-LEB1

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Plux2

Available since

Oct. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pILGFP4L

Plasmid

#104507

Purpose

Used to evaluate the expression output of Saccharomyces mikatae GAL2 promoter with yEGFP used as the reporter gene

Depositor

Claudia Vickers

Article

Peng et al ACS Synth Biol. 2018 Feb 16;7(2):748-751

Insert

SmGAL2 promoter-yEGFP

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Nov. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pBUN279

Plasmid

#60674

Purpose

integration of M. smegmatis ald gene in Mycobacterium using mycobacteriophage L5 attB site

Depositor

Raul Barletta

Article

Feng et al J Bacteriol. 2002 Sep;184(18):5001-10.

Insert

ald

Use

E.coli-mycobacterium integration-proficient vector

Tags

Expression

Mutation

Promoter

Available since

July 6, 2015

Availability

Academic Institutions and Nonprofits only
vector-dendra2 PCDNA

Plasmid

#121134

Purpose

mammalian cell expression

Depositor

Qing Deng

Article

Hsu et al Proc Natl Acad Sci U S A. 2019 Aug 26. p

Insert

empty cassete for contol of miR expression

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pICH42222

Plasmid

#50328

Depositor

Nicola Patron, Sylvestre Marillonnet

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

CDS, phosphinothricin acetyl transferase, (S. hygroscopicus)

Use

Plant expression

Tags

Expression

Mutation

BsaI/BbsI sites removed by point-mutation; See De…

Promoter

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pILGFP4H

Plasmid

#104503

Purpose

Used to evaluate the expression output of Saccharomyces arboricola GAL1 promoter with yEGFP used as the reporter gene

Depositor

Claudia Vickers

Article

Peng et al ACS Synth Biol. 2018 Feb 16;7(2):748-751

Insert

SaGAL1 promoter-yEGFP

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Sept. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pILGFP4J

Plasmid

#104505

Purpose

Used to evaluate the expression output of Saccharomyces mikatae GAL1 promoter with yEGFP used as the reporter gene

Depositor

Claudia Vickers

Article

Peng et al ACS Synth Biol. 2018 Feb 16;7(2):748-751

Insert

SmGAL1 promoter-yEGFP

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Sept. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pILGFP4N

Plasmid

#104509

Purpose

Used to evaluate the expression output of Saccharomyces bayanus GAL2 promoter with yEGFP used as the reporter gene

Depositor

Claudia Vickers

Article

Peng et al ACS Synth Biol. 2018 Feb 16;7(2):748-751

Insert

SbGAL2 promoter-yEGFP

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Sept. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pILGFP4R

Plasmid

#104513

Purpose

Used to evaluate the expression output of Saccharomyces uvarum GAL2 promoter with yEGFP used as the reporter gene

Depositor

Claudia Vickers

Article

Peng et al ACS Synth Biol. 2018 Feb 16;7(2):748-751

Insert

SuGAL2 promoter-yEGFP

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Sept. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pTK_Sox10_enh_99a_Citrine

Plasmid

#130589

Purpose

enhancer reporter

Depositor

Tatjana Sauka-Spengler

Article

Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

Insert

Sox10_enh_99a

Use

Enhancer reporter

Tags

Expression

Mutation

Promoter

thymidine kinase minimal promoter

Available since

Nov. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pHT-trxA.ads

Plasmid

#47383

Purpose

Expresses ads with trxA tag under Pgrac promoter (inducible by IPTG) in bacteria

Depositor

Heng-Phon Too

Article

Zhou et al Biotechnol Bioeng. 2013 Mar 8. doi: 10.1

Insert

ads

Use

Tags

6xHis and trxA

Expression

Bacterial

Mutation

Promoter

Pgrac

Available since

Oct. 10, 2013

Availability

Academic Institutions and Nonprofits only

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pGGF008

Plasmid

#48848

Purpose

Provides a BASTA resistance cassette as GreenGate module.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Insert

pNOS::BastaR::tNOS

Use

Golden gate compatible cloning vector

Tags

Expression

Mutation

chi sequence in BastaR removed by silent substitu…

Promoter

Available since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pET-32M human DKK1c (183–266)

Plasmid

#132626

Purpose

expresses human DKK1c (183–266) in E. coli

Depositor

Wenqing Xu

Article

Cheng et al Nat Struct Mol Biol. 2011 Oct 9;18(11):1

Insert

DKK1 (183–266)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pTK_TFAP2a_enh_249_Cerulean

Plasmid

#130615

Purpose

enhancer reporter

Depositor

Tatjana Sauka-Spengler

Article

Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

Insert

TFAP2a_enh_249

Use

Enhancer reporter

Tags

Expression

Mutation

Promoter

thymidine kinase minimal promoter

Available since

Nov. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pTK_FoxD3_enh_372_Citrine

Plasmid

#130583

Purpose

enhancer reporter

Depositor

Tatjana Sauka-Spengler

Article

Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

Insert

FoxD3_enh_372

Use

Enhancer reporter

Tags

Expression

Mutation

Promoter

thymidine kinase minimal promoter

Available since

Nov. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pICSL70005

Plasmid

#50335

Depositor

Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

bialaphos resistance cassette (Nos promoter (A. tumefaciens) + phosphinothricin acetyl transferase (S. hygroscopicus) + Nos term

Use

Plant expression

Tags

Expression

Mutation

BsaI/BbsI sites removed by point-mutation

Promoter

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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