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Plasmid#105171Purposeubiquitin C promoter drives rtTA3 expressionDepositorInsertUbCp-rtTA3
UseLentiviral and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
NmCas9_sgRNA_expression_in_pBluescript
Plasmid#122091PurposeU6 driven NmCas9 sgRNA expression vector for cloning own guidesDepositorInsertNmCas9 tracrRNA (NEWENTRY Synthetic, S. pyogenes)
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceApril 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJRA107
Plasmid#209340PurposeGateway cloning compatible binary vector for agrobacterium mediated transformation. Arabidopsis UBQ10 promoter expression of N-terminal mNeonGreen fusion protein.DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceJan. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLH-sgRNA-Sirius-8XPP7
Plasmid#121940PurposeCRISPR-Sirius plasmidDepositorInsertsgRNA-Sirius-8XPP7
UseCRISPR and LentiviralTagsnoExpressionMammalianMutationPromoterhuman U6 promoterAvailable sinceFeb. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
JH04
Plasmid#73513PurposemRuby2_PH pCFJ150 - contains C. elegans codon optimized mRuby2DepositorInsertpmex-5::mRuby2::PH::tbb-2
UseTagsmRuby2ExpressionMutationPromotermex-5Available sinceFeb. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
RhoC FLARE.sc mCer, mVenus - wt
Plasmid#65071PurposeRhoC biosensor single chain; mCerulean-tagged ROCK-RBD, mVenus-tagged RhoCDepositorInsertRBD, mCerulean, mVenus, RhoC
UseTagsExpressionBacterial, Insect, and Mamm…MutationT153M in mVenusPromoterCMV, p10Available sinceJuly 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
AMPK-γ1-HA
Plasmid#40605DepositorInsertAMPK-γ1 (Prkag1 Mouse)
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceSept. 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
pmScarlet_NES_C1
Plasmid#85060PurposeIn vivo visualization of the cytoplasm, but not the nucleus (can be used for colocalization studies)DepositorInsertNES
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
mNeptune-pBAD
Plasmid#54714PurposeLocalization: Protein Expression Vector , Excitation: 600, Emission: 650DepositorTypeEmpty backboneUseTags6xHis and mNeptuneExpressionBacterialMutationPromoterAvailable sinceJuly 25, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pSIN-TRE-SspB-mCh-p60
Plasmid#190170PurposeDoxycycline-inducible lentivirus vector to express SspBmicro-mCh-p60, a recruitable katanin for microtubule severing, in mammalian cells.DepositorInsertSspBmicro-mCherry-p60
UseLentiviralTagsSspBmicro-mCherryExpressionMutationPromoterAvailable sinceNov. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-myc3-NEDD8
Plasmid#19943DepositorInsertNEDD 8 (Neural precursor cell Expressed, Developmentally Down-regulated 8 (NEDD8 Human)
UseTagsmyc3ExpressionMammalianMutationPromoterAvailable sinceMay 5, 2009AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-CD9-(TEV)-tTA
Plasmid#162596Purposehuman CD9-TEV cleavage site-tTADepositorInserthuman CD9 (CD9 Human)
UseTagsTEV protease cleavage site and tTAExpressionMammalianMutationPromoterCMV promoterAvailable sinceFeb. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEMHE-mCherry-mTrim21ΔPRYSPRY
Plasmid#105524PurposeT7 promotor drives in vitro transcription of mCherry-tagged mouse TRIM21 with deleted PRYSPRY domain mRNADepositorInsertTRIM21 (Trim21 Mouse)
UseIn vitro transcriptionTagsmCherryExpressionMutationdeletion of PRYSPRY domain (aa1-281)PromoterT7Available sinceMarch 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLX302 GDF11-V5 puro
Plasmid#83097PurposeLentiviral vector for constitutive expression of human GDF11 with C-terminal V5 tagDepositorInsertGDF11 (GDF11 Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterAvailable sinceFeb. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCRISPaint-T2A-Gal4DBD-3xP3-RFP
Plasmid#127564PurposeCRISPaint universal donor plasmid for gene exon insertion. Encodes T2A-Gal4DBD-SV40 and 3xP3-RFP visible eye marker.DepositorInsertGal4DBD
UseCRISPRTagsExpressionInsectMutationPromoterAvailable sinceJuly 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HA+WT GADD45
Plasmid#24929DepositorInsertGADD45 (GADD45A Human)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceJune 10, 2010AvailabilityAcademic Institutions and Nonprofits only -
mc2 mCherry EF1a
Plasmid#206225PurposeFor expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceAug. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCRISPaint-T2A-p65-3xP3-RFP
Plasmid#127565PurposeCRISPaint universal donor plasmid for gene exon insertion. Encodes T2A-p65-SV40 and 3xP3-RFP visible eye marker.DepositorInsertp65
UseCRISPRTagsExpressionInsectMutationPromoterAvailable sinceOct. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCK760
Plasmid#192643PurposeExpresses BBa_J23110-sfGFP (capacity monitor) and J3-Bba_J23117-mRFP (CRISPRa reporter) on pSC101-AmpRDepositorInsertBBa_J23110-sfGFP
UseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterBBa_J23110Available sinceJan. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAGM65879
Plasmid#153214PurposeLevel 2 cloning vector for one or several guide RNAs, already contains Cas9 and Bar selection cassetteDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only