Showing: 4221 - 4240 of 13929 results
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pETM11-6xHis-TEV-hORF1p_R261A
Plasmid#202586PurposeAllows for IPTG-inducible expression of the human L1RP ORF1 protein with the R261A mutations in bacteria with an N-terminal 6xHis tag and TEV cleavage site for purification with a minimal scarDepositorInsertORF1p
UseTags6x His and TEV protease cleavage siteExpressionBacterialMutationChanged ORF1p Arginine 261 to AlaninePromoterAvailabilityAcademic Institutions and Nonprofits only -
pETM11-6xHis-TEV-hORF1p_StammerAEA
Plasmid#202588PurposeAllows for IPTG-inducible expression of the human L1RP ORF1 protein with the M91A and L93A mutations in bacteria with an N-terminal 6xHis tag and TEV cleavage site for purificationDepositorInsertORF1p
UseTags6x His and TEV protease cleavage siteExpressionBacterialMutationChanged ORF1p Methionine 91 to Alanine, changed O…PromoterAvailabilityAcademic Institutions and Nonprofits only -
p15a_AcCas12n
Plasmid#203806Purposep15a-based plasmid containing a rpsL driven AcCas12n cassette and the araBAD-driven lambda-red recombinase cassette. Apramycin resistance.DepositorInsertAcCas12n
UseCRISPRTagsExpressionMutationPromoterrpslAvailabilityAcademic Institutions and Nonprofits only -
pSGKP_AcsgRNA
Plasmid#203807PurposepSGKP-based plasmid containing a J23119-driven sgRNA cassette, with replaceable spacer (BsaI-restriction sites). Kanamycin resistance.DepositorInsertAcgRNA
UseCRISPRTagsExpressionMutationPromoterJ23119AvailabilityAcademic Institutions and Nonprofits only -
pUDP293
Plasmid#204228PurposeEpisomal yeast plasmid expressing the Ercas12a and ErCas12a gRNADepositorInsertsEubacterium rectale cas12a (Ercas12a)
BsaI_GFP drop-out_BsaI
UseSynthetic BiologyTagsNLSExpressionMutationcodon optimized for S. cerevisiaePromoterSaccharomyces cerevisiae PGK1 and Saccharomyces c…AvailabilityAcademic Institutions and Nonprofits only -
pV132-vCas9
Plasmid#203223PurposeMammalian expression of vCas9DepositorInsertvCas9
UseAAV and CRISPRTagsExpressionMammalianMutationS55R R976A K1003A T1314RPromoterCBhAvailabilityAcademic Institutions and Nonprofits only -
His-DERA
Plasmid#203225PurposeFor protein expression. E. coli 2-deoxy-D-ribose-5-phosphate aldolase. Converts dR5P to G3P and acetaldehyde. Pet28a plasmid, KAN resistant, N-terminal TEV-cleavable 6xHis tag.DepositorInsertdeoxyribose aldolase
UseTagsN-terminal TEV cleavable 6xHis tagExpressionBacterialMutationPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pDisplay-NE2m-IRES-mCherry-CAAX
Plasmid#208690PurposeExpresses the genetically-encoded fluorescent norepinephrine (NE) sensor GRAB_NE2m and a membrane-localized mcherry in mammalian cellsDepositorInsertGPCR activation based norepinephrine (NE) sensor GRAB_NE2m
UseTagsExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pDisplay-NE2h-IRES-mCherry-CAAX
Plasmid#208691PurposeExpresses the genetically-encoded fluorescent norepinephrine (NE) sensor GRAB_NE2h and a membrane-localized mcherry in mammalian cellsDepositorInsertGPCR activation based norepinephrine (NE) sensor GRAB_NE2h
UseTagsExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-DIO-GRAB_g5-HT2h
Plasmid#208715PurposeExpresses the green 5-HT sensor GRAB_g5-HT2h in neurons in the presence of Cre recombinaseDepositorInsertGreen fluorescent 5-HT sensor GRAB_g5-HT2h
UseAAVTagsExpressionMutationPromoterhSynAvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-GRAB_g5-HT2m
Plasmid#208716PurposeExpresses the green 5-HT sensor GRAB_g5-HT2m in neuronsDepositorInsertGreen fluorescent 5-HT sensor GRAB_g5-HT2m
UseAAVTagsExpressionMutationPromoterhSynAvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-GRAB_g5-HT3.0
Plasmid#208717PurposeExpresses the green 5-HT sensor GRAB_g5-HT3.0 in neuronsDepositorInsertGreen fluorescent 5-HT sensor GRAB_g5-HT3.0
UseAAVTagsExpressionMutationPromoterhSynAvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-GRAB_g5-HT3.0mut
Plasmid#208718PurposeExpresses the green 5-HT sensor GRAB_g5-HT3.0mut in neuronsDepositorInsertGreen fluorescent 5-HT sensor GRAB_g5-HT3.0mut
UseAAVTagsExpressionMutationPromoterhSynAvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-GRAB_r5-HT1.0
Plasmid#208719PurposeExpresses the red 5-HT sensor GRAB_r5-HT1.0 in neuronsDepositorInsertRed fluorescent 5-HT sensor GRAB_r5-HT1.0
UseAAVTagsExpressionMutationPromoterhSynAvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-GRAB_r5-HTmut
Plasmid#208720PurposeExpresses the red 5-HT sensor GRAB_r5-HTmut in neuronsDepositorInsertRed fluorescent 5-HT sensor GRAB_r5-HTmut
UseAAVTagsExpressionMutationPromoterhSynAvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-GRAB_g5-HT2h
Plasmid#208721PurposeExpresses the green 5-HT sensor GRAB_g5-HT2h in neuronsDepositorInsertGreen fluorescent 5-HT sensor GRAB_g5-HT2h
UseAAVTagsExpressionMutationPromoterhSynAvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-DIO-GRAB_g5-HT2m
Plasmid#208722PurposeExpresses the green 5-HT sensor GRAB_g5-HT2m in neurons in the presence of Cre recombinaseDepositorInsertGreen fluorescent 5-HT sensor GRAB_g5-HT2m
UseAAVTagsExpressionMutationPromoterEF1aAvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKII-GRAB_g5-HT3.0
Plasmid#208723PurposeExpresses the green 5-HT sensor GRAB_g5-HT3.0 in excitatory neuronsDepositorInsertGreen fluorescent 5-HT sensor GRAB_g5-HT3.0
UseAAVTagsExpressionMutationPromoterCaMKIIAvailabilityAcademic Institutions and Nonprofits only
Showing: 4221 - 4240 of 13929 results