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Plasmid#177959PurposeTransient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (P80R, R203K, G204R). Used for generating RNA packaging virus-like particles.DepositorInsertSARS-CoV-2 Nucleocapsid (P80R, R203K, G204R) (N )
UseTagsExpressionMammalianMutationP80R,R203K,G204RPromoterAvailable sinceNov. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
CoV2-N-B.1.1.7
Plasmid#177958PurposeTransient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (D3L, R203K, G204R, S235F). Used for generating RNA packaging virus-like particles.DepositorInsertSARS-CoV-2 Nucleocapsid (D3L, R203K, G204R, S235F) (N )
UseTagsExpressionMammalianMutationD3L,R203K,G204R,S235FPromoterAvailable sinceNov. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HLA-cMyc-EcopT1R1
Plasmid#113962Purposemammalian expression plasmid for c-myc-tagged E. coli codon-optimized human T1R1 with signal peptide of HLA class I histocompatibility antigen A-2 alpha chainDepositorInsertT1R1 (TAS1R1 Human)
UseTagsSignal/leader sequence from HLA class I histocomp…ExpressionMammalianMutationtruncate N-terminal 24 residuesPromoterAvailable sinceFeb. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
InsRA-EBFP2
Plasmid#79797PurposeInsulin receptor isoform A with a inter-domain EBFP2 tag at extracellular region (between furin-like domain and transmembrane domain). For imaging insulin receptor trafficing.DepositorInsertInsRA (INSR Human)
UseTagsEBFP2 extracellularExpressionMammalianMutationPromoterCMVAvailable sinceAug. 5, 2016AvailabilityIndustry, Academic Institutions, and Nonprofits -
NSP14-Flag
Plasmid#210346PurposeExpresses SARS-CoV-2 NSP14 fused with Flag in mammalian cellsDepositorInsertSARS-CoV-2 NSP14 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1, Human)
UseTagsFlagExpressionMammalianMutationMany synonymous changes due to codon optimizationPromoterAvailable sinceJan. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
HiBiT-FZD4
Plasmid#195842PurposeExpresses human FZD4 with an N-terminal HiBiTDepositorInsertFZD4 (FZD4 Human)
UseTagsHiBiTExpressionMammalianMutationPromoterCMVAvailable sinceMarch 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
HiBiT-FZD6
Plasmid#195844PurposeExpresses human FZD6 with an N-terminal HiBiT; contains M345LDepositorInsertFZD6 (FZD6 Human)
UseTagsHiBiTExpressionMammalianMutationM345LPromoterCMVAvailable sinceMarch 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
BNLS
Plasmid#50836PurposeExpresses a tandem ddFP heterodimer in mammalian cells, construct 1 for a translocation-based green fluorescent caspase-3 biosensor, used together with plasmid 1 (GANLS-DEVD-BNES or RANLS-DEVD-BNES).DepositorInsertddGFP B
UseTagstriplicated NLS sequence DPKKKRKVExpressionMammalianMutationPromoterCMVAvailable sinceJan. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
GANLS
Plasmid#50852PurposeExpresses one ddFP in mammalian cells, the second plasmid for a red to green colour switch-based fluorescent caspase-3 biosensor, used together with plasmid NESRA-DEVD-BNLS.DepositorInsertddGFP A
UseTagstriplicated NLS sequence DPKKKRKVExpressionMammalianMutationPromoterCMVAvailable sinceJan. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
SpyTag-RBD
Plasmid#159999PurposeExpresses SARS-CoV-2 RBD (340-538) (receptor-binding domain from Spike) with N-terminal SpyTag fusion in mammalian cellsDepositorInsertSpyTag-SARS-CoV-2 RBD (340-538) (S SARS-CoV-2)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
HiBiT-FZD2
Plasmid#195840PurposeExpresses human FZD2 with an N-terminal HiBiTDepositorInsertFZD2 (FZD2 Human)
UseTagsHiBiTExpressionMammalianMutationPromoterCMVAvailable sinceMay 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
Opto-GPR151
Plasmid#106051PurposeExpression of a chimeric G-protein coupled receptor (Rhodopsin-GPR151; Opto-GPR151; E5)DepositorInsertOpto-GPR151 (GPR151 Bovine, Human)
UseTagsRhodopsin-1D4 and VSV-GExpressionMammalianMutationChimeric receptor proteinPromoterCMVAvailable sinceJan. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-sACE2(WT)
Plasmid#145147PurposeMammalian expression plasmid for soluble ACE2 (protease domain)DepositorInsertAngiotensin-converting enzyme 2 (ACE2 Human)
UseTagsExpressionMammalianMutationExtracellular, soluble protease domain (a.a. M1-D…PromoterCMVAvailable sinceApril 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
WN10150
Plasmid#80443PurposeExpresses dead (908A) AsCpf1DepositorInsertnuclease dead (908A) AsCpf1
UseTagsNLS-3xHAExpressionMammalianMutationnuclease dead (D908A)PromoterAvailable sinceAug. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
CMV-mito-CAR-GECO1
Plasmid#46022PurposeCarmine intensiometric genetically encoded Ca2+-indicators for optical imagingDepositorInsertCAR-GECO1
UseTagsa duplex of the mitochondrial targeting signal of…ExpressionMammalianMutationR-GECO1 E163V/I166V/V174T/M176I/F222I/A302PPromoterCMVAvailable sinceJuly 23, 2013AvailabilityAcademic Institutions and Nonprofits only -
ITSN-mcherry-sspB
Plasmid#85220PurposeRole of Cdc-42 selective GEF domain from ITSN in immune cell migrationDepositorInsertsUseTagsNAExpressionMammalianMutationR73QPromoterCMVAvailable sinceJan. 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
Arrb1-Q394L
Plasmid#35402DepositorInsertbeta-arrestin-1 (Arrb1 Rat)
UseTagsFlagExpressionMammalianMutationQ394LPromoterCMVAvailable sinceMarch 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-IP3KA
Plasmid#32529DepositorInsertIP3KA (ITPKA Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 4, 2012AvailabilityAcademic Institutions and Nonprofits only -
pcDNA 3.1 LIC 6A NLRP1-FLAG S1213A
Plasmid#166804PurposeMammalian cell expression vector for FLAG-tagged NLRP1. The S1213A mutant is deficient in FIIND autoprocessingDepositorInsertNLRP1 (NLRP1 Human)
UseTagsFLAGExpressionMammalianMutationS1213APromoterCMVAvailable sinceMarch 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
hHSF1 S303/7A
Plasmid#118365PurposeThis plasmid expresses human HSF1 S303A and S307A mutant, which cannot undergo sumoylation on K298 due to disrupted PDSM motif.DepositorInserthHSF1 (HSF1 Human)
UseTagsmyc and 6xHISExpressionMammalianMutationS303A and S307APromoterAvailable sinceNov. 8, 2018AvailabilityAcademic Institutions and Nonprofits only