opto-E-cad GFP
(Plasmid #203327)

• Purpose: Expresses E-cadherin with a LOV2 insert after T133 for blue light inhibition in mammalian cells
• Depositing Lab: Seraphine Wegner
• Publication: Nzigou Mombo et al Nat Commun. 2023 Oct 9;14(1):6292. doi: 10.1038/s41467-023-41932-0.

Backbone

• Vector backbone: pcDNA3.1
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Photoswitchable - E-cadherin with GFP tag
• Alt name: opto-E-cad
• Species: H. sapiens (human)
• Insert Size (bp): 3783
• Mutation: Addition of Aslov2 domain at T133 of human E-cadherin
• GenBank ID:
• Entrez Gene: CDH1 (a.k.a. Arc-1, BCDS1, CD324, CDHE, ECAD, LCAM, UVO)
• Tag / Fusion Protein:
  • GFP (C terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: GTGATGGAGGTCACAGCCACACTGGCGACCACCCTGGAACG
• 3′ sequencing primer: GTTCACATCATCGTCCGCGTCCGCTTCATCAATGTTTTCCG

Resource Information

• Supplemental Documents:
  • opto-E-cad DNA sequence.gbk
• A portion of this plasmid was derived from a plasmid made by: The E-cadherin GFP plasmid was a gift from Jennifer Stow, Addgene plasmid #28009 The AsLOV2 domain was a gift from Brian Kuhlman and amplified from Addgene plasmid #40236

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry