-
Plasmid#127140DepositorInsertRPS3_Keima (RPS3 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJuly 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLJM1 Flag Raptor
Plasmid#26633DepositorInsertraptor (RPTOR Human)
UseLentiviralTagsFlagExpressionMammalianMutationPromoterAvailable sinceOct. 22, 2010AvailabilityAcademic Institutions and Nonprofits only -
PP7_mCherry
Plasmid#61763PurposeExpresses PP7 coat protein fused to mCherryDepositorInsertPP7 coat protein fused to mCherry
UseLentiviralTagsmCherryExpressionMammalianMutationPromoterUbc promoterAvailable sinceFeb. 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBAM1
Plasmid#60487PurposeThis plasmids is a modular mini-Tn5 vector that could be used to generate random mutagenesis libraries or to insert heterologous DNA into a target genomeDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceOct. 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSR33.4r
Plasmid#63198PurposeExpresses Cph8, Ho1, and PcyA constitutivelyDepositorInsertsCph8
Ho1
PcyA
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceApril 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLenti-HIFR
Plasmid#192946PurposeLentiviral production of HIF-1alpha reporterDepositorInsertHIF-1alpha reporter
UseLentiviralTagsExpressionMutationPromotermCMVAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-3xFLAG-TEV-UBE2O
Plasmid#105718PurposeExpresses WT full length human N- terminally tagged UBE2ODepositorInsertUBE2O (UBE2O Human)
UseTags3xFLAG-TEVExpressionMammalianMutationPromoterCMV (from vector)Available sinceFeb. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAG1247 H2B-ECFP-FIREmate
Plasmid#205171PurposeExpresses H2B-ECFP-FIREmate in mammalian cellsDepositorInsertH2B-ECFP-FIREmate
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
-
U6-AsCas12f-full-length-sgRNA
Plasmid#204638PurposeExpression of full-length AsCas12f sgRNA in mammalian cellsDepositorInsertfull-length AsCas12f sgRNA
UseTagsExpressionMammalianMutationPromoterU6Available sinceAug. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO APEX2-GFP
Plasmid#129640PurposeExpress APEX2-GFP fusion in mammalian cellsDepositorInsertAPEX2-GFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pESC-URA-GFP-Ubc9ts
Plasmid#20369DepositorInsertSUMO-conjugating enzyme Ubc9 (UBC9 Budding Yeast)
UseYeast/bacteria shuttleTagsGFPExpressionYeastMutationY68L This mutation renders Ubc9 temperature-sens…PromoterAvailable sinceMay 28, 2009AvailabilityAcademic Institutions and Nonprofits only -
pCDH_blast_MCS_Nard_GFP_D50
Plasmid#167339PurposeExpresses GFP fused to progerin (d50) in mammalian cellsDepositorInsertProgerin
UseLentiviralTagsGFPExpressionMammalianMutation50AA deletion creating Progerin instead of mature…PromoterCMVAvailable sinceApril 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
PcDNA3.1-Flag
Plasmid#210342PurposeEmpty Vector for molecular cloningDepositorInsertFlag tag
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEMT-PTE2A
Plasmid#111773PurposeQuadcistronic construct: Co-express four genes by three 2A peptides -- P2A, T2A and E2ADepositorTypeEmpty backboneUseCloning intermediateTagsExpressionMutationPromoterT7Available sinceAug. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEX-6P-1 Stu2 1-590
Plasmid#38314DepositorInsert5' 1770 bp from STU2 ORF (STU2 Budding Yeast)
UseTagsGSTExpressionBacterialMutationPromotertacAvailable sinceAug. 17, 2012AvailabilityAcademic Institutions and Nonprofits only -
pFastBac His6 TEV cloning vector with BioBrick PolyPromoter LIC Subcloning (438-B)
Plasmid#55219DepositorTypeEmpty backboneUseTagsHis6ExpressionInsectMutationPromoterAvailable sinceJune 13, 2014AvailabilityAcademic Institutions and Nonprofits only -
pME-mTagBFP
Plasmid#75148PurposeTagBFP (blue) fluorophore for use with 5’ promoters.DepositorInsertmTagBFP
UseMiddle entry vector for multisite gateway three f…TagsExpressionMutationPromotern/aAvailable sinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBT374
Plasmid#125615PurposeExpresses evoFERNY-BE4max-NG in mammalian cellsDepositorInsertevoFERNY-BE4max-NG
UseTagsExpressionMammalianMutationH102P D104NPromoterAvailable sinceJuly 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTW199b2
Plasmid#136928PurposeDirected evolution of SpCas9DepositorInsertgIII
UseCRISPRTagsExpressionBacterialMutationnonePromoterAvailable sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only