Skip to main content
Addgene
Showing: 6301 - 6330 of 148509 results

  4. pXPR_219

    Plasmid
    #164559
    Purpose
    Lentiviral vector that encodes two sgRNA expression cassettes. Also encodes the fluorophore violet-excited GFP (Vex) as a marker of transduction.
    Depositor
    Arlene Sharpe
    Article
    LaFleur et al Nat Immunol. 2023 Nov 27. doi: 10.1038/s
    Inserts
    First sgRNA cassette
    Second sgRNA cassette
    Use
    CRISPR and Lentiviral; Dual sgrna
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Human U6 and Mouse U6
    Available since
    Nov. 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    164559-plasmid-map-sequence-id-330205

    Enlarge

  10. mEmerald-KLC2-N-18

    Plasmid
    #62759
    Purpose
    Localization: Kinesin Light Chain , N1 Cloning Vector, Excitation:487, Emission:509
    Depositor
    Michael Davidson
    Insert
    KLC2
    Use
    Tags
    mEmerald
    Expression
    Mammalian
    Mutation
    none
    Promoter
    CMV
    Available since
    March 10, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  12. Human NCKX5 (pcDNA3.1+)

    Plasmid
    #75214
    Purpose
    Mammalian expression of SLC24A5
    Depositor
    Jonathan Lytton
    Insert
    SLC24A5 (SLC24A5 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    June 21, 2016
    Availability
    Academic Institutions and Nonprofits only
    75214-plasmid-map-sequence-id-147898

    Enlarge

  15. pAS_4xMma PylT_FLAG-AcKRS

    Plasmid
    #140021
    Purpose
    Plasmid with 4xMmaPylT cassette and MmaPylRS for amber suppression and incorporation of acetyl-lysine; for transient or stable piggyBac-mediated integration
    Depositor
    Simon Elsaesser
    Article
    Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006
    Insert
    AcKRS-3 (pylS Methanosarcina mazei)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    L301M, L305I, Y306F, L309A, C348F
    Promoter
    EF1
    Available since
    May 20, 2020
    Availability
    Academic Institutions and Nonprofits only
    140021-plasmid-map-sequence-id-297663

    Enlarge

  21. TFORF3520

    Plasmid
    #144996
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    TCF4 (TCF4 Human)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    144996-plasmid-map-sequence-id-287102

    Enlarge

  27. BRD4

    Plasmid
    #38943
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    BRD4 (BRD4 Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only
Showing: 6301 - 6330 of 148509 results