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pCMV-PB1-AG-FRB-TEVuncs-mTagBFP2-HA

Plasmid

#178869

Purpose

Mammalian expression of a protein of interest fused to the C-terminus of PB1-AzamiGreen-FRB-TEVuncs-mTagBFP-HA

Depositor

Shinya Tsukiji

Article

Yoshikawa et al J Am Chem Soc. 2021 May 5;143(17):6434-6

Insert

PB1-AzamiGreen-FRB-TEVuncs(NEYLQFL)-mTagBFP2-HA

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-mCherry-Zdk1-Vav2cat

Plasmid

#178875

Purpose

Mammalian expression of Vav2(DH-PH-CRD) fused to mCherry-Zdk1

Depositor

Shinya Tsukiji

Article

Yoshikawa et al J Am Chem Soc. 2021 May 5;143(17):6434-6

Insert

mCherry-Zdk1-Vav2(DH-PH-CRD) (Vav2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 10, 2022

Availability

Academic Institutions and Nonprofits only

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CAG.mCherry-INBox-eDHFR

Plasmid

#176202

Purpose

Single INBox (INCENP 819-918) recruitment responsible for targeted Aurora B activation and recruitment using TMP-Halo dimerization system

Depositor

Michael Lampson

Article

Chen et al J Cell Biol. 2021 Jun 7;220(6). pii: 212

Insert

mCherry-INBox-eDHFR

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

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CAG.mCherry-INBox(AAA)-eDHFR

Plasmid

#176207

Purpose

INBox TSS(892-894)-mutant replaced by AAA responsible for targeted Aurora B recruitment without activation using TMP-Halo dimerization system

Depositor

Michael Lampson

Article

Chen et al J Cell Biol. 2021 Jun 7;220(6). pii: 212

Insert

mCherry-INBoxAAA-eDHFR

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 24, 2021

Availability

Academic Institutions and Nonprofits only

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TetO::EGFP-hMeikin(8A mut)

Plasmid

#174707

Purpose

Doxycycline inducible expression of human Meikin with 8 alanine mutations that eliminate Plk1 binding

Depositor

Iain Cheeseman

Article

Maier et al Dev Cell. 2021 Aug 9;56(15):2192-2206.e8

Insert

Meikin

Use

Tags

EGFP

Expression

Mammalian

Mutation

S175A, T176A, T180A, S181A, S196A, T251A, T264A, …

Promoter

Available since

Oct. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pJH776_pJH_LldR_PlldR-sfGFP.3opal

Plasmid

#163735

Purpose

Lactate-inducible expression of green fluorescent protein encoding three opal codons

Depositor

Matthew Bennett

Article

Ho et al Nucleic Acids Res. 2021 Mar 18;49(5):e25

Insert

lldr gfp.3TGA

Use

Tags

Expression

Bacterial

Mutation

gfp with first three leucine codons mutated to am…

Promoter

Available since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pJH775_pJH_LldR_PlldR-sfGFP.2opal

Plasmid

#163734

Purpose

Lactate-inducible expression of green fluorescent protein encoding two opal codons

Depositor

Matthew Bennett

Article

Ho et al Nucleic Acids Res. 2021 Mar 18;49(5):e25

Insert

lldr gfp.2TGA

Use

Tags

Expression

Bacterial

Mutation

gfp with first two leucine codons mutated to amber

Promoter

Available since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-FLAG-PGRMC1-1-170

Plasmid

#173467

Purpose

PGRMC1 lacking the extreme C-terminus

Depositor

Bill Tsai

Article

Chen et al Nat Commun. 2021 Oct 13;12(1):5991. doi:

Insert

PGRMC1 (PGRMC1 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Deleted for amino acids 171-194

Promoter

Available since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-HA-PGRMC1-1-54

Plasmid

#173472

Purpose

PGRMC1 lacking the C-terminal region downstream of the transmembrane domain

Depositor

Bill Tsai

Article

Chen et al Nat Commun. 2021 Oct 13;12(1):5991. doi:

Insert

PGRMC1 (PGRMC1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Deleted for amino acids 55-194

Promoter

Available since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-HA-PGRMC1-21-195

Plasmid

#173473

Purpose

PGRMC1 lacking the N-terminal region upstream of the transmembrane region

Depositor

Bill Tsai

Article

Chen et al Nat Commun. 2021 Oct 13;12(1):5991. doi:

Insert

PGRMC1 (PGRMC1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Deleted for amino acids 1-20

Promoter

Available since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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IBMc120

Plasmid

#161950

Purpose

Contains elements for building reporter plasmid for IBM. Built upon pSB4A3. ECF20 reporter plasmid

Depositor

Baojun Wang

Article

Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:

Insert

pSB4A3-PECF20-B30-mScarlet-I-B15

Use

Tags

Expression

Bacterial

Mutation

Promoter

PECF20

Available since

March 23, 2021

Availability

Academic Institutions and Nonprofits only

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IBMc393

Plasmid

#161953

Purpose

Demonstrates chemically induced dimmerization of acVHH. T7 RNAP polymerase reporter plasmid

Depositor

Baojun Wang

Article

Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:

Insert

pSB4A3-T-PT7-B30-mScarlet-I-3T

Use

Tags

Expression

Bacterial

Mutation

Promoter

PT7

Available since

March 16, 2021

Availability

Academic Institutions and Nonprofits only

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IBMc330

Plasmid

#161952

Purpose

Contains elements for building reporter plasmid for IBM. SrpR reporter plasmid

Depositor

Baojun Wang

Article

Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:

Insert

pSEVA171-PSrpR-B30-mScarlet-I-Ter

Use

Tags

Expression

Bacterial

Mutation

Promoter

PSrpR

Available since

March 14, 2021

Availability

Academic Institutions and Nonprofits only

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IBMc050

Plasmid

#161938

Purpose

Carries Golden Gate substitution insert for IBM with M86 intein. Plux2 as C-lobe promoter

Depositor

Baojun Wang

Article

Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:

Insert

pSB1A3-BbsI-M86N-cat-Plux2-B32-M86C-SapI

Use

Tags

Expression

Bacterial

Mutation

Promoter

Plux2

Available since

March 14, 2021

Availability

Academic Institutions and Nonprofits only

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IBMc259

Plasmid

#161951

Purpose

Contains elements for building reporter plasmid for IBM.TetR reporter plasmid

Depositor

Baojun Wang

Article

Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:

Insert

pSEVA471-PTet*-RiboJ10-B64-mScarlet-I-Ter

Use

Tags

Expression

Bacterial

Mutation

Promoter

PTet*

Available since

March 14, 2021

Availability

Academic Institutions and Nonprofits only

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Nup54 no FG-mEGFP494(2)

Plasmid

#164271

Purpose

Mammalian expression of Nup54 without the FG-Nup domain, with mEGFP (missing the first 5 amino acids) at amino acid position 494 with a rigid alpha helix of 7 amino acids at the carboxyl end of mEGFP

Depositor

Sanford Simon

Article

Pulupa et al Elife. 2020 Dec 21;9. pii: 60654. doi: 1

Insert

Nup54 (NUP54 Human)

Use

Tags

mEGFP

Expression

Mammalian

Mutation

deleted the FG region of Nup54

Promoter

CMV

Available since

Feb. 23, 2021

Availability

Academic Institutions and Nonprofits only

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Nup54 no FG-mEGFP494(1)

Plasmid

#164270

Purpose

Mammalian expression of Nup54 without the FG-Nup domain, with mEGFP (missing the first 5 amino acids) at amino acid position 494 with a rigid alpha helix of 6 amino acids at the carboxyl end of mEGFP

Depositor

Sanford Simon

Article

Pulupa et al Elife. 2020 Dec 21;9. pii: 60654. doi: 1

Insert

Nup54 (NUP54 Human)

Use

Tags

mEGFP

Expression

Mammalian

Mutation

deleted the FG region of Nup54

Promoter

CMV

Available since

Feb. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pCbh-SpyCas9-2A-puro-pA_U6-sgRosa26

Plasmid

#149346

Purpose

Mammalian expression vector for SpyCas9, puromycin resistance and mouse Rosa26 guide RNA

Depositor

Ralf Kuehn

Article

Danner et al Open Biol. 2021 Jan;11(1):200283. doi: 1

Insert

Cas9

Use

CRISPR

Tags

Flag tag

Expression

Mammalian

Mutation

Promoter

Cbc

Available since

Jan. 27, 2021

Availability

Academic Institutions and Nonprofits only

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pCbh-SpyCas9-2A-puro-pA_U6-BbsI

Plasmid

#149347

Purpose

Mammalian expression vector for SpyCas9 and puromycin resistance

Depositor

Ralf Kuehn

Article

Danner et al Open Biol. 2021 Jan;11(1):200283. doi: 1

Insert

SpyCas9

Use

CRISPR

Tags

Flag tag

Expression

Mammalian

Mutation

Promoter

Cbh

Available since

Jan. 27, 2021

Availability

Academic Institutions and Nonprofits only

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pCRII-TOPO CMV-cGFP-SV40 pA + Laccase2 Exon 2

Plasmid

#91802

Purpose

Expresses the Laccase2 circular RNA when the upstream SV40 poly(A) signal fails to be used

Depositor

Jeremy Wilusz

Article

Liang et al Methods. 2021 Apr 18. pii: S1046-2023(21

Insert

Coral Green Fluorescent Protein (cGFP)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 31, 2020

Availability

Academic Institutions and Nonprofits only

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