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Plasmid#202528PurposeExpress C-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-tagged human Sphingomyelin synthase 1 (SMS1) in mammalian cellDepositorInsertSGMS1 (SGMS1 Human)
UseTagsTEV cleavable site and Twin-Strep-tagExpressionMammalianMutationdeleted stop codon (TAA)PromoterCAG and chicken β-actin promoterAvailable sinceSept. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pIRES-hrGFP II-mTET1
Plasmid#83569PurposeExpresses TET1 with a mutated catalytic domain in mammalian cellsDepositorInsertTet Methylcytosine Dioxygenase 1 (TET1 Human)
UseTags3X FLAG tag and hr GFP IIExpressionMammalianMutationcatalytic domain mutant H1672D, D1674APromoterCMVAvailable sinceSept. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1.H1-NP
Plasmid#134367PurposeNanoluc complementation assay. Expression of histamine receptor H1 fused at C termimus with Natural peptide (NP) of NanoLuc. Addition of the Flag epitope at N terminus of H1 receptor.DepositorInsertH1-NP (HRH1 Human)
UseTagsFlag and natural peptide of nanoluciferaseExpressionBacterial and MammalianMutationPromoterT7Available sinceJuly 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pGW011b Dual AAV vector pAAV-EFS-dCAS9-spA
Plasmid#192160PurposeDual AAV vector AAV-dCas9DepositorInsertDead Cas9
UseAAVTagsExpressionMutationNAPromoterAvailable sinceOct. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFL His-SDS22 / PP1gamma
Plasmid#113511PurposeExpression of His-SDS22 / PP1gamma dimer in insect cells (Baculovirus system)DepositorUseTags6*HisExpressionInsectMutationPromoterAvailable sinceOct. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
STARR-seq luciferase validation vector_ORI_SV40
Plasmid#99309PurposeLuciferase validation vector with SV40 enhancer inserted downstream of the reporter gene. The candidate's enhancer activity is reflected by luciferase activityDepositorInsertSV40 enhancer
UseLuciferase; Starr-seq luciferase validation vectorTagsExpressionMammalianMutationPromoterAvailable sinceSept. 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pNCST-mAvicFP1
Plasmid#129509PurposeExpresses mAvicFP1 constitutively in E. coli (most strains)DepositorInsertmAvicFP1
UseTagsExpressionBacterialMutationPromotersynthetic constitutive (stationary phase) promote…Available sinceFeb. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFL His-mEos3.2-I3
Plasmid#113516PurposeExpression of His-Eos-I3 in insect cells (Baculovirus system)DepositorInsertI3 (PPP1R11 Human)
UseTags6*His-mEos3.2ExpressionInsectMutationPromoterAvailable sinceOct. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
STARR-seq luciferase INF enhancer vector_ORI_IFIT2
Plasmid#99321PurposeLuciferase validation vector with IFIT2 enhancer inserted downstream of the reporter gene. The candidate's enhancer activity is reflected by luciferase activityDepositorInsertenhancer; chr10: 91060605-91062447
UseLuciferase; Starr-seq luciferase validation vectorTagsExpressionMammalianMutationPromoterAvailable sinceAug. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMV-dCas9-D3A
Plasmid#78256PurposeExpresses dead Cas9 (dCas9) fused to DNMT3A catalytic domain (CD) under the control of the CMV promoterDepositorInsertDNMT3A CD aa 598-912 (DNMT3A Human)
UseCRISPRTagsFlag epitope tagExpressionMammalianMutationPromoterpCMVAvailable sinceJune 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
EGFP-Rab7a-7
Plasmid#56443PurposeLocalization: Ras GTPase, Excitation: 488, Emission: 507DepositorInsertRab7a (RAB7A Human)
UseTagsEGFPExpressionMammalianMutationN117S in NM_004637.5PromoterCMVAvailable sinceJan. 13, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pRS313_ATP3
Plasmid#120253PurposeExpression of ATP3 in yeastDepositorInsertATP3 (ATP3 Budding Yeast)
UseTagsExpressionYeastMutationPromoterATP3Available sinceApril 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCfB3052(gRNA X-4, XI-3, XII-5)
Plasmid#73294PurposeEasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at sites X-4, XI-3, and XII-5DepositorInsertguiding RNA
UseCRISPR; GrnaTagsExpressionYeastMutationPromoterAvailable sinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
MAC-TMPRSS4
Plasmid#172422PurposeMAC-tagged gene expressionDepositorInsertTransmembrane protease serine 4 (TMPRSS4 Human)
UseTagsMAC-tagExpressionMammalianMutationPromoterCMV promoterAvailable sinceNov. 4, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
FpG5
Plasmid#69443Purposelentiviral GFP reporter plasmid for high throughput functional detection of active transcriptional regulatory DNAsDepositorInsertsminimal FGF4 promoter
EGFP
Ubiquitin promoter
Hygromycin resistance
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMay 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCMV-GABRA1(A322D)-IRES2-EGFP
Plasmid#170821Purposehuman GABAA receptors alpha1 subunit carrying A322D mutation and IRES2-EGFPDepositorInsertHuman GABAA receptor alpha1 subunit carrying A322D mutation (GABRA1 Human)
UseTagsIRES2-EGFPExpressionMammalianMutationchange Alanine 322 to AspartatePromoterAvailable sinceAug. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
Venus-BimEL-pEGFP-C1
Plasmid#166735PurposeExpress Venus fused to the N-terminus of the Bcl-2 family protein, BimELDepositorInsertBimEL (Bcl2l11 Mouse)
UseTagsVenusExpressionMammalianMutationPromoterCMVAvailable sinceMarch 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
P119 C-Check
Plasmid#66817PurposeDual-fluorescent surrogate reporter vector for in vitro functional assay of programable DNA nuclease and enrichment of genetically edited cellsDepositorInserttruncated Enhanced Green Fluorescent Protein and asRED
UseTagsExpressionMammalianMutationPromoterPGK and CMVAvailable sinceJan. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSICO-CPSF6-358-eGFP
Plasmid#110693PurposeExpresses a truncated form of CPSF6 (CPSF6-358 originally described in Lee et al. Cell Host Microbe 2010) fused with eGFPDepositorInsertCPSF6-eGFP (CPSF6 )
UseLentiviralTagsEGFPExpressionMutationTruncation of CPSF6 isoform 2 at amino acid 358PromoterAvailable sinceJuly 25, 2018AvailabilityAcademic Institutions and Nonprofits only