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Addgene

EGFR G719S
(Plasmid #11013)

Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 11013 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pBABE puro
  • Backbone manufacturer
    available at Addgene
  • Backbone size w/o insert (bp) 5169
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    EGFR G719S
  • Alt name
    epidermal growth factor receptor
  • Alt name
    ERBB
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3639
  • Mutation
    G719S
  • Entrez Gene
    EGFR (a.k.a. ERBB, ERBB1, ERRP, HER1, NISBD2, NNCIS, PIG61, mENA)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SnaBI (destroyed during cloning)
  • 3′ cloning site SnaBI (destroyed during cloning)
  • 5′ sequencing primer pBABE5'
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Author has deposited the sequence of the insert to Addgene. Click on "sequence" to view. Point mutation made by using the following primers to PCR from plasmid 11011: 5'-AAGATCAAAGTGCTGAGCTCCGGTGCGTTCG-3' and 5'-CGAACGCACCGGAGCTCAGCACTTTGATCTT-3'

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    EGFR G719S was a gift from Matthew Meyerson (Addgene plasmid # 11013 ; http://n2t.net/addgene:11013 ; RRID:Addgene_11013)
  • For your References section:

    Oncogenic Transformation by Inhibitor-Sensitive and -Resistant EGFR Mutants. Greulich H, Chen TH, Feng W, Janne PA, Alvarez JV, Zappaterra M, Bulmer SE, Frank DA, Hahn WC, Sellers WR, Meyerson M. PLoS Med. 2005 Oct 4. 2(11):e313. 10.1371/journal.pmed.0020313 PubMed 16187797