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Plasmid#138693PurposeExpresses a human NUAK2-targeting sgRNA and Cas9DepositorInsertsgNUAK2 human (NUAK2 Human)
UseLentiviralTagsExpressionMutationPromoterhU6Available sinceFeb. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLenti-FoxO1-Clover
Plasmid#67759PurposeFluorescent fusion protein for tracking Akt signalingDepositorInsertFox01 (Foxo1 Mouse)
UseLentiviralTagsCloverExpressionMutationChanged Serine 209 to Alanine, changed Histidine …PromoterEF1aAvailable sinceAug. 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLenti-PE2-BSD
Plasmid#161514PurposeExpression of PE2 in mammalian cells. Can be used to generate stable cell lines by Blasticidin selectionDepositorInsertPE2-P2A-BSD
UseLentiviralTagsSV40 NLSExpressionMammalianMutationPromoterEF-1α core promoterAvailable sinceNov. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLenti6.2-Nanoluc-ccdB
Plasmid#87078PurposeLentiviral Gateway-compatible expression vector backbone with an N-terminal Nanoluc luciferaseDepositorTypeEmpty backboneUseLentiviralTagsNanoluc luciferaseExpressionMammalianMutationPromoterAvailable sinceMarch 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLenti6.2-ccdB-Nanoluc
Plasmid#87075PurposeLentiviral Gateway-compatible expression vector backbone with a C-terminal Nanoluc luciferaseDepositorTypeEmpty backboneUseLentiviralTagsNanoluc luciferaseExpressionMammalianMutationPromoterAvailable sinceMarch 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLentiCrispr-V2-mOrange (V2mO)
Plasmid#140206PurposeLenti Crispr/Cas9 with mOrange fluorescent markerDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterpELF1aAvailable sinceMarch 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
Plenti-ARE-HSVTK-luc
Plasmid#161789PurposeExpresses HSV-TK and Luciferase in an NRF2 dependent manner. Also, expresses cherry and blasticidin resistance selection markersDepositorInsertsLuciferase-T2A-HSV-TK
mCherry P2A blasticidin
UseLentiviral and LuciferaseTagsExpressionMammalianMutationPromoterEF1a and Murine Gclc AREAvailable sinceFeb. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
plentiCMV-sp-HPV-TM(ICAM)
Plasmid#196510PurposeExpress HPV antigen epitope with ICAM transmembrane domainDepositorInserta HPV epitope with CD8a stalk linker and ICAM TM domain
UseLentiviralTags3xflag tagExpressionMammalianMutationPromoterCMVAvailable sinceMarch 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti-nSpCas9-TadA8e
Plasmid#209044PurposeLentiviral vector expressing doxycycline-inducible human codon-optimized adenine base editor where TadA8e (ABE8e) is fused to the N-terminus of SpCas9(D10A)-6xNLSDepositorInsertTadA8e-nSpCas9
UseCRISPR and LentiviralTagsFLAG-HAExpressionMammalianMutationD10APromoterTight TRE promoterAvailable sinceJune 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti-opCas12a-6xNLS
Plasmid#209022PurposeLentiviral vector expressing human codon-optimized OpCas12a with 6xNLSDepositorInsertopCas12a-6xNLS-Flag-Myc-NeoR
UseCRISPR and LentiviralTagsFLAG and MYCExpressionMammalianMutationE174R/S542RPromoterEF-1aAvailable sinceJune 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti6-V5-Topo mCherry
Plasmid#128062PurposeLentiviral vector for C terminal mCherry fusionsDepositorTypeEmpty backboneUseLentiviralTagsmCherryExpressionMammalianMutationPromoterAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLenti-mCherry-CDK4KTR
Plasmid#126680PurposeFluorescent reporter for CDK4/6 activityDepositorInsertRB cterm
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceApril 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLenti-hSyn-nucGFP
Plasmid#140190PurposeOverexpression of GFP with a nuclear localization sequence, under the human synapsin promoterDepositorInsertGFP-NLS
UseLentiviralTagsGFPExpressionMammalianMutationPromoterAvailable sinceJuly 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLenti_dCas9-KRAB-MeCP2_Blast
Plasmid#221501PurposeLentiviral construct for expression of the dCas9-KRAB-MeCP2 CRISPRi vectorDepositorInsertdCas9-KRAB-MeCP2-T2A-Blast
UseCRISPR and LentiviralTagsExpressionMammalianMutationCas9 mutations to make dCas9=D10A+D839A+H840A+N86…PromoterCMVAvailable sinceJuly 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti6/V5-DEST-ANLN
Plasmid#31203DepositorInsertANLN (ANLN Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterAvailable sinceJuly 29, 2011AvailabilityAcademic Institutions and Nonprofits only -
pLenti6/UbC/mSlc7a1
Plasmid#17224DepositorInsertSlc7a1 (Slc7a1 Mouse)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 8, 2008AvailabilityAcademic Institutions and Nonprofits only -
plenti-X1-Neo-RPN1-HA
Plasmid#139868PurposeLentiviral expression of HA-tagged (C terminal) human RPN1DepositorInsertRPN1 (Ribophorin1) (RPN1 Human)
UseLentiviralTagsHAExpressionMutationPromoterAvailable sinceJuly 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
plenti-FLAG-CAMK2D-ED
Plasmid#221700PurposeLentiviral expression of FLAG-tagged human CAMK2D (K43R/D136N) in mammalian cellsDepositorInsertCAMK2D (CAMK2D Human)
UseLentiviralTagsExpressionMutationK43R, D136NPromoterAvailable sinceAug. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti_gRNA -hMLHdn-Puro
Plasmid#191105PurposeBackbone for lentiviral pegRNA library construction with hMLH1dn and Puro markerDepositorInserthMLH1dn
UseCRISPR and LentiviralTagsExpressionMammalianMutationI31 silent mutation (ATA to ATC) to remove early …PromoterEF-1aAvailable sinceMay 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLentiCRISPRv2-sgAAVS1-HepmTurquoise2
Plasmid#192828PurposeExpresses sgRNA targeting AAVS1 (non-targeting control sgRNA for mouse) and mTurquoise2 from hepatocyte-specific promoterDepositorInsertmTurquoise2
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6 for sgRNA and hepatocyte-specific promoter (HS…Available sinceNov. 23, 2022AvailabilityAcademic Institutions and Nonprofits only