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Showing: 401 - 420 of 562 results

  1. pAAV-hSyn-DIO {mCAR}off-{ChETA-mRuby2}on-W3SL

    Plasmid
    #111390
    Purpose
    AAV vector with hSynapsin promoter, Cre-OFF mCAR (for efficient CAV-2 infection), Cre-ON ChETA-mRuby2 (for optogenetic activation), and W3SL regulatory cassette (for maximize cloning capacity)
    Depositor
    Adam Kepecs
    Article
    Li et al Neuron. 2018 Jun 6;98(5):905-917.e5. doi
    Inserts
    mCAR (Cxadr Mouse)
    ChETA-mRuby2
    Use
    AAV
    Tags
    Myc and mRuby2
    Expression
    Mammalian
    Mutation
    Promoter
    hSynapsin
    Available since
    June 8, 2018
    Availability
    Academic Institutions and Nonprofits only
    111390-plasmid-map-sequence-id-212377

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  2. pAAV-hSyn1-Flex-mRuby2-GSG-P2A-GCaMP6s-WPRE-pA

    Plasmid
    #68720
    Purpose
    Cre-dependent bicistronic vector expressing mRuby2 and GCaMP6s from a single open reading frame.
    Depositor
    Mark Huebener, Tobias Bonhoeffer, Tobias Rose
    Article
    Rose et al Science. 2016 Jun 10;352(6291):1319-22.
    Has Service
    AAV1
    Insert
    mRuby2-P2A-GCaMP6s
    Use
    AAV
    Tags
    Expression
    Mutation
    Cre-dependent expression from inverted open readi…
    Promoter
    hSyn1-FLEX
    Available since
    June 20, 2016
    Availability
    Academic Institutions and Nonprofits only
    68720-plasmid-map-sequence-id-198989

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  3. pAAV-hSyn-DIO {hCAR}off-{ChETA-mRuby2}on-W3SL

    Plasmid
    #111391
    Purpose
    AAV vector with hSynapsin promoter, Cre-OFF hCAR (for efficient CAV-2 infection), Cre-ON ChETA-mRuby2 (for optogenetic activation), and W3SL regulatory cassette (for maximize cloning capacity)
    Depositor
    Adam Kepecs
    Article
    Li et al Neuron. 2018 Jun 6;98(5):905-917.e5. doi
    Inserts
    hCAR (CXADR Human)
    ChETA-mRuby2
    Use
    AAV
    Tags
    Myc and mRuby2
    Expression
    Mammalian
    Mutation
    Promoter
    hSynapsin
    Available since
    June 7, 2018
    Availability
    Academic Institutions and Nonprofits only
    111391-plasmid-map-sequence-id-212240

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  5. pUC19_mRuby-3xFLAG-NLS-3xSV40-poly(A)_loxP-PGK-Puro-loxP

    Plasmid
    #195505
    Purpose
    T-REX17 donor plasmid to generate locus specific integration of mRuby followed by 3xSV40 poly(A) into Exon 1 of human lncRNA T-REX17
    Depositor
    Alexander Meissner
    Article
    Landshammer et al Elife. 2023 Jan 31;12:e83077. doi: 10.75
    Insert
    pT-REX-mRuby-3xFLAG-NLS-3xSV40-poly(A)_loxP-PGK-Puro-loxP_T-REX(Ex1)
    Use
    CRISPR; Donor vector
    Tags
    mRuby-3xFLAG-NLS-3xSV40-poly(A)
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    195505-plasmid-map-sequence-id-384991

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  7. pAU003 - pTRE3G-Cterm_Nterm_switch_CTCF-mRuby2-CAGGS-rtta3G-Frt-PGK-PuroR-bpA-Frt TIGRE

    Plasmid
    #156430
    Purpose
    Vector to introduce a constitutive rtta3G cassette and a DOX-inducible mutant CTCF mouse cDNA, targeting the mouse TIGRE acceptor locus. Use with Addgene #92144 sgRNA. Use puromycine selection.
    Depositor
    Elphege Nora
    Article
    Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:
    Insert
    CTCF, rtta3G, TetO3G (Ctcf Mouse)
    Use
    Mouse Targeting
    Tags
    Expression
    Mammalian
    Mutation
    Cterm_Nterm_switched
    Promoter
    Available since
    Sept. 3, 2020
    Availability
    Academic Institutions and Nonprofits only
    156430-plasmid-map-sequence-id-310203

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  8. pEN515 - pTRE3G-CTCF(ZFmut)-mRuby2-CAGGS-rtta3G-Frt-PGK-PuroR-bpA-Frt TIGRE

    Plasmid
    #156434
    Purpose
    Vector to introduce a constitutive rtta3G cassette and a DOX-inducible mutant CTCF mouse cDNA (all Zinc fingers point mutated), targeting the mouse TIGRE acceptor locus. Use with Addgene #92144 sgRNA.
    Depositor
    Elphege Nora
    Article
    Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:
    Insert
    CTCF, rtta3G, TetO3G (Ctcf Mouse)
    Use
    Mouse Targeting
    Tags
    Expression
    Mammalian
    Mutation
    All zinc fingers mutated
    Promoter
    Available since
    Sept. 3, 2020
    Availability
    Academic Institutions and Nonprofits only
    156434-plasmid-map-sequence-id-310270

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  9. pAU002 - pTRE3G-CTCF(DELTA1-265)-mRuby2-CAGGS-rtta3G-Frt-PGK-PuroR-bpA-Frt TIGRE

    Plasmid
    #156429
    Purpose
    Vector to introduce a constitutive rtta3G cassette and a DOX-inducible mutant CTCF mouse cDNA, targeting the mouse TIGRE acceptor locus. Use with Addgene #92144 sgRNA. Use puromycine selection.
    Depositor
    Elphege Nora
    Article
    Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:
    Insert
    CTCF, rtta3G, TetO3G (Ctcf Mouse)
    Use
    Mouse Targeting
    Tags
    Expression
    Mammalian
    Mutation
    DELTA1-265
    Promoter
    Available since
    Sept. 3, 2020
    Availability
    Academic Institutions and Nonprofits only
    156429-plasmid-map-sequence-id-310209

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  10. pEN366 - pTRE3G-CTCF-mRuby2-BGHpA-CAGGS-rtta3G-rbgpA-Frt-PGK-EM7-PuroR-bpA-Frt TIGRE donor

    Plasmid
    #156432
    Purpose
    Vector to introduce a constitutive rtta3G cassette and a DOX-inducible wild-type CTCF mouse cDNA, targeting the mouse TIGRE acceptor locus. Use with Addgene #92144 sgRNA. Use puromycine selection.
    Depositor
    Elphege Nora
    Article
    Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:
    Insert
    CTCF, rtta3G, TetO3G (Ctcf Mouse)
    Use
    Mouse Targeting
    Tags
    Expression
    Mammalian
    Mutation
    none
    Promoter
    Available since
    Sept. 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    156432-plasmid-map-sequence-id-310156

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  12. pDEW8

    Plasmid
    #84238
    Purpose
    Plasmid containing a P2A peptide (porcine teschovirus-1 derived) sequence that works efficiently in Saccharomyces cerevisiae
    Depositor
    David Weinberg
    Article
    DiSanto et al Elife. 2016 Oct 1;5. pii: e20069. doi: 1
    Insert
    HA-mRuby-P2A-eGFP
    Use
    Tags
    HA-mRuby and eGFP
    Expression
    Yeast
    Mutation
    Promoter
    GPD
    Available since
    Nov. 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    84238-plasmid-map-sequence-id-164972

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  13. pKL004

    Plasmid
    #98920
    Purpose
    Constitutively expresses the calcium sensor GCaMP6f tethered to mRuby3 under the Sigma 70 promoter in E. coli.
    Depositor
    Joel Kralj
    Article
    Bruni et al Proc Natl Acad Sci U S A. 2017 Aug 29;11
    Insert
    GCaMP6f tethered to mRuby
    Use
    Tags
    His and mRuby3
    Expression
    Bacterial
    Mutation
    Promoter
    BBa_J23118 - Strong Constitutive
    Available since
    May 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    98920-plasmid-map-sequence-id-189873

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  17. MTK0_057

    Plasmid
    #123950
    Purpose
    Encodes the hAAVS1 BxBI recognition site with Hygromycin resistance and mRuby2 landing pad with GFP dropout destination vector as a type 0 part to be used in the MTK system
    Depositor
    Hana El-Samad
    Article
    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2
    Insert
    BxBI LP on hAAVS1 (Hygro::mRuby)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    123950-plasmid-map-sequence-id-271459

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  18. MTK0_052

    Plasmid
    #123978
    Purpose
    Encodes the PhiC31 landing pad on hThal5.10-2-hg18 with hygromycin resistance cassette coexpressed with mRuby2 as a type 0 part to be used in the MTK system
    Depositor
    Hana El-Samad
    Article
    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2
    Insert
    PhiC31 LP on hThal5.10-2-hg18 (Hygro::mRuby)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    123978-plasmid-map-sequence-id-251837

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  19. MTK0_054

    Plasmid
    #123980
    Purpose
    Encodes the PhiC31 landing pad on hCLYBL with hygromycin resistance cassette coexpressed with mRuby2 as a type 0 part to be used in the MTK system
    Depositor
    Hana El-Samad
    Article
    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2
    Insert
    PhiC31 LP on hCLYBL (Hygro::mRuby)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    123980-plasmid-map-sequence-id-251849

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Showing: 401 - 420 of 562 results