Addgene

We narrowed to 2,257 results for: PRK

Showing: 601 - 630 of 2257 results

Results per page:

Sort results by:

pRK5-PLA2G15-6xHis S198A

Plasmid

#213605

Purpose

Expresses PLA2G15-6xHis S198A in mammalian cells

Depositor

Monther Abu-Remaileh

Article

Nyame et al Mol Cell. 2024 Apr 4;84(7):1354-1364.e9.

Insert

Phospholipase A2 Group XV (PLA2G15 Human)

Use

Tags

6xHis

Expression

Mammalian

Mutation

Changed serine 198 to alanine

Promoter

CMV

Available since

March 6, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pRK5-FLAG-Sestrin2-L261A

Plasmid

#73671

Purpose

overexpression

Depositor

David Sabatini

Article

Wolfson et al Science. 2016 Jan 1;351(6268):43-8. doi:

Insert

Sesn2 (SESN2 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

L261A changed Leucine 261 to Alanine

Promoter

CMV

Available since

March 30, 2016

Availability

Academic Institutions and Nonprofits only
pRK5-myc-Miro1 K427V

Plasmid

#47892

Purpose

Expresses myc tagged Miro1 K427 constitutively active mutant

Depositor

Pontus Aspenström

Article

Fransson et al Biochem Biophys Res Commun. 2006 Jun 2;3

Insert

Miro1 K427V (RHOT1 Human)

Use

Tags

myc

Expression

Mammalian

Mutation

K427V, constitutively active mutant

Promoter

CMV

Available since

Sept. 12, 2013

Availability

Academic Institutions and Nonprofits only
pRK5-mEGFP-FOXC1-MUT

Plasmid

#194567

Purpose

Mammalian Expression of mEGFP-FOXC1 Mutant (Q200Rfs*109) Fusion Protein

Depositor

Denes Hnisz

Article

Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

Insert

FOXC1 (FOXC1 Human)

Use

Tags

mEGFP

Expression

Mammalian

Mutation

Q200Rfs*109

Promoter

Available since

Feb. 22, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pRK5-mEGFP-HMGB1-delIDR

Plasmid

#194554

Purpose

Mammalian Expression of mEGFP-HMGB1 WT Fusion Protein, "del IDR" variant

Depositor

Denes Hnisz

Article

Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

Insert

HMGB1 (HMGB1 Human)

Use

Tags

mEGFP

Expression

Mammalian

Mutation

truncation by stop codon after Asn134

Promoter

Available since

Feb. 22, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
p667-UBC-PRKCQ-V5-miniTurbo_IDG-K

Plasmid

#170590

Purpose

Gateway destination clone of PRKCQ (human) tagged with C-terminal V5-miniTurbo for generating protein-proximity networks using proximity-dependent biotinylation proteomics

Depositor

Ben Major

Insert

PRKCQ-V5-miniTurbo (PRKCQ Human)

Use

Lentiviral; Gateway destination

Tags

V5-miniTurbo

Expression

Mammalian

Mutation

Promoter

Ubiquitin

Available since

Sept. 1, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pRK5-Flag CASTOR1 I280A

Plasmid

#84495

Purpose

overexpression of CASTOR1 I280A mutant

Depositor

David Sabatini

Article

Chantranupong et al Cell. 2016 Mar 24;165(1):153-64. doi: 10

Insert

CASTOR1 I280A (GATSL3 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

I280A

Promoter

CMV

Available since

Oct. 24, 2016

Availability

Academic Institutions and Nonprofits only
pRK5-FLAG-Sestrin2-E451A

Plasmid

#73673

Purpose

overexpression

Depositor

David Sabatini

Article

Wolfson et al Science. 2016 Jan 1;351(6268):43-8. doi:

Insert

Sesn2 (SESN2 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

E451A changed Glutamate 451 to Alanine

Promoter

CMV

Available since

March 30, 2016

Availability

Academic Institutions and Nonprofits only
pRK5 Flag-RagA(Q66L)

Plasmid

#99716

Purpose

Mammalian expression of Flag-RagA(Q66L)

Depositor

David Sabatini, Kuang Shen

Article

Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

Insert

RagA (RRAGA )

Use

Tags

Flag

Expression

Mutation

Q66L

Promoter

CMV

Available since

Nov. 3, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pRK5-SNAP-CLIP-GB1a

Plasmid

#125709

Purpose

Eukaryotic expression of a fusion protein encoding SNAP, CLIP and the GB1a subunit of the metabotropic GABA receptor

Depositor

Kai Johnsson

Article

Masharina et al J Am Chem Soc. 2012 Nov 21;134(46):19026

Insert

SNAP-CLIP-GB1a

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 17, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
C terminal Flag Mios pRK5

Plasmid

#46326

Depositor

David Sabatini

Article

Bar-Peled et al Science. 2013 May 31;340(6136):1100-6. d

Insert

Mios (MIOS Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

July 15, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pRK5-FLAG-Sestrin1 isoform1

Plasmid

#72594

Purpose

overexpression, codon optimized for mammalian expression

Depositor

David Sabatini

Article

Chantranupong et al Cell Rep. 2014 Oct 9;9(1):1-8. doi: 10.1

Insert

SESN1 (SESN1 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

codon optimized for mammalian expression, see dep…

Promoter

CMV

Available since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only
pRK Smad2 deltaC Flag

Plasmid

#12624

Depositor

Rik Derynck

Article

Zhang et al Nature. 1996 Sep 12. 383(6596):168-72.

Insert

Smad2 deltaC (SMAD2 Human)

Use

Tags

flag

Expression

Mammalian

Mutation

C-terminal truncated Smad2 (aa 1-424)

Promoter

Available since

Oct. 10, 2006

Availability

Academic Institutions and Nonprofits only
pRK Smad3 deltaC Flag

Plasmid

#12626

Depositor

Rik Derynck

Article

Zhang et al Nature. 1996 Sep 12. 383(6596):168-72.

Insert

Smad3 (SMAD3 Human)

Use

Tags

flag

Expression

Mammalian

Mutation

C-terminal truncated Smad3 (aa 1-382)

Promoter

Available since

Oct. 10, 2006

Availability

Academic Institutions and Nonprofits only
pRK-flag-USP19 C506S

Plasmid

#78584

Purpose

Expression flag-USP19 C506S in mammalian cells

Depositor

Yihong Ye

Article

Lee et al Nat Cell Biol. 2016 Jul;18(7):765-76. do

Insert

flag-USP19 C506S (USP19 Human)

Use

Tags

flag

Expression

Mammalian

Mutation

mutate 506 cysteine residue to serine

Promoter

CMV

Available since

June 22, 2016

Availability

Academic Institutions and Nonprofits only
pRK-flag-USP19 1-1290

Plasmid

#78579

Purpose

Expression flag-USP19 1-1290 in mammalian cells

Depositor

Yihong Ye

Article

Lee et al Nat Cell Biol. 2016 Jul;18(7):765-76. do

Insert

flag-USP19 1-1290 (USP19 Human)

Use

Tags

flag

Expression

Mammalian

Mutation

mutate 1291 residue to stop condon

Promoter

CMV

Available since

June 22, 2016

Availability

Academic Institutions and Nonprofits only
pRK5-FLAG-RagC-D181N

Plasmid

#42325

Depositor

David Sabatini

Article

Bar-Peled et al Cell. 2012 Sep 14;150(6):1196-208. doi:

Insert

RagC D181N (RRAGC Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

D181N

Promoter

CMV, SP6

Available since

March 6, 2013

Availability

Academic Institutions and Nonprofits only
pRK5-FLAG-RagB-D163N

Plasmid

#42324

Depositor

David Sabatini

Article

Bar-Peled et al Cell. 2012 Sep 14;150(6):1196-208. doi:

Insert

RagB D163N (RRAGB Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

D163N

Promoter

CMV, SP6

Available since

March 6, 2013

Availability

Academic Institutions and Nonprofits only
pRK5 Flag-RagC(Q120L)

Plasmid

#99725

Purpose

Mammalian expression of Flag-RagC(Q120L)

Depositor

David Sabatini, Kuang Shen

Article

Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

Insert

RagC (RRAGC )

Use

Tags

Flag

Expression

Mutation

Q120L

Promoter

CMV

Available since

Nov. 3, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pRK5 GFP RagB Q99L

Plasmid

#112749

Purpose

express GFP-RagB (Q99L mutant)

Depositor

Roberto Zoncu

Article

Lawrence et al Nat Cell Biol. 2018 Sep;20(9):1052-1063.

Insert

GFP-RagB (Q99L) (RRAGB Human)

Use

Tags

eGFP

Expression

Mammalian

Mutation

Q99L

Promoter

Available since

Oct. 15, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pRK5-Myc-TEAD4-Y429H

Plasmid

#33041

Depositor

Kunliang Guan

Article

Li et al Genes Dev. 2010 Feb 1;24(3):235-40.

Insert

TEAD4 (TEAD4 Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

Y429H

Promoter

Available since

March 28, 2012

Availability

Academic Institutions and Nonprofits only
pRK5 Flag-RagA(T21N)

Plasmid

#99715

Purpose

Mammalian expression of Flag-RagA(T21N)

Depositor

David Sabatini, Kuang Shen

Article

Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

Insert

RagA (RRAGA )

Use

Tags

Flag

Expression

Mutation

T21N

Promoter

CMV

Available since

Nov. 3, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
HA GST p85 S6K1 pRK5

Plasmid

#8466

Depositor

David Sabatini

Article

Ali et al J Biol Chem 2005 Apr 4;.

Insert

p85 s6k1 (Rps6kb1 Rat)

Use

Tags

ha/gst

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 14, 2005

Availability

Academic Institutions and Nonprofits only
pMXs-Puro- MLL3-PRKAG2

Plasmid

#69473

Purpose

Expresses MLL3-PRKAG2 fusion gene

Depositor

Axel Hillmer

Article

Yao et al Cell Rep. 2015 Jul 14;12(2):272-85. doi:

Insert

MLL3-PRKAG2 (PRKAG2 Human)

Use

Retroviral

Tags

HA TAG:

Expression

Mutation

Promoter

Available since

Nov. 13, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pRK-Myc-Smurf1 delHECT

Plasmid

#13677

Depositor

Ying Zhang

Article

Yamashita et al Cell. 2005 Apr 8. 121(1):101-13.

Insert

Smurf1 deltaHect (SMURF1 Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

Smurf1 without HECT domain (deleted amino acids 3…

Promoter

Available since

Feb. 8, 2007

Availability

Academic Institutions and Nonprofits only
pRK5 CASTOR2-Flag Cterm

Plasmid

#84490

Purpose

overexpression of CASTOR2 with Flag tag

Depositor

David Sabatini

Article

Chantranupong et al Cell. 2016 Mar 24;165(1):153-64. doi: 10

Insert

CASTOR2 (GATSL2 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 24, 2016

Availability

Academic Institutions and Nonprofits only
pRK5-myc-Miro1 S432N

Plasmid

#47893

Purpose

Expresses myc tagged Miro1 S432N dominant negative mutant

Depositor

Pontus Aspenström

Article

Fransson et al Biochem Biophys Res Commun. 2006 Jun 2;3

Insert

Miro1 S432N (RHOT1 Human)

Use

Tags

myc

Expression

Mammalian

Mutation

S432N, dominant negative mutant

Promoter

CMV

Available since

Sept. 12, 2013

Availability

Academic Institutions and Nonprofits only
pRK5-mEGFP-RAX-MUT

Plasmid

#194573

Purpose

Mammalian Expression of mEGFP-RAX Mutant (S222Rfs*63) Fusion Protein

Depositor

Denes Hnisz

Article

Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

Insert

RAX (RAX Human)

Use

Tags

mEGFP

Expression

Mammalian

Mutation

S222Rfs*63

Promoter

Available since

Feb. 22, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pRK5-mEGFP-MYOD1-MUT

Plasmid

#194571

Purpose

Mammalian Expression of mEGFP-MYOD1 Mutant (R188Pfs*90) Fusion Protein

Depositor

Denes Hnisz

Article

Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

Insert

MYOD1 (MYOD1 Human)

Use

Tags

mEGFP

Expression

Mammalian

Mutation

R188Pfs*90

Promoter

Available since

Feb. 22, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pRK5-myc-PICK1-rescue

Plasmid

#72573

Purpose

Expresses myc-PICK1 shRNA resistant cDNA

Depositor

Victor Anggono, Richard Huganir

Article

Anggono et al J Neurosci. 2011 Feb 9;31(6):2188-96. do

Insert

PICK1 (Pick1 Rat)

Use

Tags

Myc

Expression

Mammalian

Mutation

5 silent mutations around shRNA recognition seque…

Promoter

CMV

Available since

Jan. 3, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge