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KBB353

Plasmid

#185081

Purpose

Swi6M-GFP expressing N-term 181 amino acids of Swi6

Depositor

Ken Belanger

Article

Belanger et al BMC Mol Cell Biol. 2022 Feb 21;23(1):10.

Insert

SWI6

Use

Tags

GFP

Expression

Yeast

Mutation

Swi6 truncation aa 1-181

Promoter

Available since

July 21, 2022

Availability

Academic Institutions and Nonprofits only

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pEGPN

Plasmid

#187482

Purpose

Expression vector - GFP

Depositor

Lewis Cantley

Article

Kastenhuber et al Elife. 2022 Mar 23;11. pii: 77444. doi:

Insert

EGFP

Use

Lentiviral

Tags

Expression

Mutation

Promoter

EFS

Available since

July 20, 2022

Availability

Academic Institutions and Nonprofits only

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KBB484

Plasmid

#185132

Purpose

SWI6-GFP full length under control of GAL1 promotion

Depositor

Ken Belanger

Article

Belanger et al BMC Mol Cell Biol. 2022 Feb 21;23(1):10.

Insert

SWI6

Use

Tags

GFP

Expression

Yeast

Mutation

Promoter

Available since

July 19, 2022

Availability

Academic Institutions and Nonprofits only

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KBB486

Plasmid

#185133

Purpose

Full length CRM1 fused to binding domain of two-hybrid plasmid

Depositor

Ken Belanger

Article

Belanger et al BMC Mol Cell Biol. 2022 Feb 21;23(1):10.

Insert

CRM1

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

July 19, 2022

Availability

Academic Institutions and Nonprofits only

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KBB491

Plasmid

#185134

Purpose

Full length SWI6 fused to activation domain of two-hybrid plasmid

Depositor

Ken Belanger

Article

Belanger et al BMC Mol Cell Biol. 2022 Feb 21;23(1):10.

Insert

SWI6

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

July 19, 2022

Availability

Academic Institutions and Nonprofits only

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pVH9-Tier1-PhCMV-3xNLS

Plasmid

#169478

Purpose

Tier-1 vector encoding PhCMV-driven triple nuclear localization signal expression (PhCMV-3xNLS-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PCMV-driven nuclear localization signal

Use

Tags

Expression

Mammalian

Mutation

Promoter

PhCMV

Available since

June 14, 2022

Availability

Academic Institutions and Nonprofits only

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pVH8-Tier1-PhCMV-NLS

Plasmid

#169477

Purpose

Tier-1 vector encoding PhCMV-driven nuclear localization signal expression (PhCMV-NLS-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PCMV-driven nuclear localization signal

Use

Tags

Expression

Mammalian

Mutation

Promoter

PhCMV

Available since

June 14, 2022

Availability

Academic Institutions and Nonprofits only

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TDRD3:ABC006-C02:C220502

Plasmid

#183982

Purpose

Baculovirus expression for structure determination. May not contain entire coding region of gene.

Depositor

Cheryl Arrowsmith

Insert

TDRD3-anti-TDRD3 FAB (TDRD3 Human)

Use

Tags

anti-TDRD3 FAB HC: N-terminal STII leader peptid…

Expression

Bacterial

Mutation

Promoter

Available since

June 6, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pHBT-Mb(GDx-Clo_L10)

Plasmid

#183406

Purpose

Expresses Monobody GDx-Clo_L10 in E. coli

Depositor

Shohei Koide

Article

Kermani et al Nat Commun. 2020 Nov 27;11(1):6064. doi:

Insert

His tag-Avi tag-TEV cleavage site-Mb(GDx Clo_L10)

Use

Tags

His tag-Avi tag-TEV cleavage site

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 25, 2022

Availability

Academic Institutions and Nonprofits only

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pYPQ-NF1-L1-dSpRY

Plasmid

#177681

Purpose

Gateway entry clone (attL1 & attR5) for dimeric FokI-dCas9-SpRY genome editing

Depositor

Yiping Qi

Article

Cheng et al Biotechnol J. 2022 Apr 4:e2100571. doi:

Insert

FokI-dCas9-SpRY

Use

CRISPR; Gateway compatible foki-dcas9-spry entry …

Tags

NLS

Expression

Plant

Mutation

Promoter

Available since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

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pYPQ-NF1-L2-dSpRY

Plasmid

#177682

Purpose

Gateway entry clone (attL1 & attR5) for dimeric FokI-dCas9-SpRY genome editing

Depositor

Yiping Qi

Article

Cheng et al Biotechnol J. 2022 Apr 4:e2100571. doi:

Insert

FokI-dCas9-SpRY

Use

CRISPR; Gateway compatible foki-dcas9-spry entry …

Tags

NLS

Expression

Plant

Mutation

Promoter

Available since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

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pYPQ-NF-L2-dMb2Cas12a

Plasmid

#177684

Purpose

Gateway entry clone (attL1 & attR5) for dimeric FokI-dMb2Cas12a genome editing

Depositor

Yiping Qi

Article

Cheng et al Biotechnol J. 2022 Apr 4:e2100571. doi:

Insert

FokI-dMb2Cas12a

Use

CRISPR; Gateway compatible foki-dmb2cas12a entry …

Tags

NLS

Expression

Plant

Mutation

Promoter

Available since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

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pBM13g-sd4U

Plasmid

#179689

Purpose

Bacterial expression of base editor

Depositor

David Liu

Article

Mok et al Nat Biotechnol. 2022 Sep;40(9):1378-1387

Insert

[sd4U] UGI-TALE3-DddA-G1397-N (S1330I + A1341V + N1342S + E1370K + T1380I)

Use

Tags

Expression

Bacterial

Mutation

S1330I A1341V N1342S E1370K T1380I

Promoter

Available since

April 4, 2022

Availability

Academic Institutions and Nonprofits only

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pnYC-NpM-NcPAN2_1-390_Y

Plasmid

#148103

Purpose

Bacterial Expression of NcPAN2_1-390

Depositor

Elisa Izaurralde

Article

Jonas et al Nat Struct Mol Biol. 2014 Jul;21(7):599-

Insert

NcPAN2_1-390

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 25, 2022

Availability

Academic Institutions and Nonprofits only

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pJH2028

Plasmid

#179771

Purpose

Pnlf-1::NLF::2XGFP unc-54 3'UTR C.elegans neural expression of NLF::2XGFP

Depositor

Mei Zhen

Article

Xie et al Neuron. 2013 Mar 20;77(6):1069-82. doi:

Insert

NLF-1::2XGFP (nlf-1 Nematode)

Use

Tags

2XGFP

Expression

Worm

Mutation

Promoter

nlf-1

Available since

March 14, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pJH2198

Plasmid

#179773

Purpose

Prgef-1 NLF-1::GFP unc-54 3' UTR C.elegans pan-neural expression of NLF-1::GFP

Depositor

Mei Zhen

Article

Xie et al Neuron. 2013 Mar 20;77(6):1069-82. doi:

Insert

NLF-1::GFP (nlf-1 Nematode)

Use

Tags

GFP

Expression

Worm

Mutation

Promoter

Prgef-1

Available since

March 14, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pAc5.1B-lambdaN-HA-DmGW182-delUBAdelQrich_G

Plasmid

#146396

Purpose

Insect Expression of DmGW182-delUBAdelQrich

Depositor

Elisa Izaurralde

Article

Zekri et al Mol Cell Biol. 2009 Sep 21. ():.

Insert

DmGW182-delUBAdelQrich (gw Fly)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 3, 2022

Availability

Academic Institutions and Nonprofits only

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pJH2437

Plasmid

#179780

Purpose

Prgef-1 NLF-1 unc-54 3' UTR C.elegans pan-neural expression of NLF

Depositor

Mei Zhen

Article

Xie et al Neuron. 2013 Mar 20;77(6):1069-82. doi:

Insert

NLF-1 (nlf-1 Nematode)

Use

Tags

no

Expression

Worm

Mutation

Promoter

Prgef-1

Available since

March 2, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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Prham-M.XhoI-M.MunI-M.Ocy1ORF8430P-p15A-aadA

Plasmid

#170768

Purpose

Methylase expression vector for 5RM assembly: expresses M.XhoI, M.MunI (MfeI) and M.Ocy1ORF8430P (SpeI) DNA methyltransferases in E. coli

Depositor

Ichiro Matsumura

Article

Matsumura et al Biotechniques. 2022 May;72(5):185-193. d

Inserts

M.XhoI
M.MunI
M.Ocy1ORF8430P

Use

Tags

Expression

Bacterial

Mutation

Promoter

rhamnose

Available since

Feb. 22, 2022

Availability

Academic Institutions and Nonprofits only

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CAG.INBox-mCherry-INBox-eDHFR

Plasmid

#176203

Purpose

Double INBox (INCENP 819-918) recruitment responsible for targeted Aurora B activation and recruitment using TMP-Halo dimerization system

Depositor

Michael Lampson

Article

Chen et al J Cell Biol. 2021 Jun 7;220(6). pii: 212

Insert

INBox-mCherry-INBox-eDHFR

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 11, 2022

Availability

Academic Institutions and Nonprofits only

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