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Plasmid#107970Purposeexpression of CD44 variantDepositorInsertmCD44v6 (Cd44 Mouse)
UseRetroviralTagsExpressionMammalianMutationsplice variant; see Depositor Comments belowPromoterAvailable sinceJan. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJZC588
Plasmid#62315PurposesgRNA with 2x MS2 (wt+f6) for yeast cellsDepositorInsertsgRNA + 2x MS2 (wt+f6) binding module
UseTagsExpressionYeastMutationPromoterSNR52Available sinceApril 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
CL20_mEGFP_KIAA1549_BRAF
Plasmid#205838PurposeExpress mEGFP-tagged fusion protein, KIAA1549_BRAF from patient-derived sequenceDepositorUseTagsmEGFPExpressionMammalianMutationPromoterAvailable sinceNov. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
nls-EGFP
Plasmid#67652PurposeMammalian expression vector driving nls-EGFP.DepositorInsertnls-EGFP
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
LV anti-FITC-LaG16-Y186A-T2A-BFP
Plasmid#194932PurposeLV construct encoding Y186A mutant sNRR-based SynNotch receptorDepositorInsertantiFITC-LaG16-sNRR(Y186A)-SynNotch-Gal4-VP64-T2A-BFP
UseLentiviral and Synthetic BiologyTagsExpressionMutationPromoterAvailable sinceMarch 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
psfGFP-LAMP1-mCherry (pHLARE)
Plasmid#164477PurposeLysosomal pH biosensor consisting of sfGFP-rat Lamp1-mCherry.DepositorInsertLAMP1 (Lamp1 Rat)
UseTagsmCherry and superfolder GFPExpressionMammalianMutationPromoterCMVAvailable sinceSept. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
EIF2AK4 gRNA (BRDN0001145948)
Plasmid#75875Purpose3rd generation lentiviral gRNA plasmid targeting human EIF2AK4DepositorInsertEIF2AK4 (EIF2AK4 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pUA66 PrpsL GFP
Plasmid#105606PurposeExpresses GFP under PrpsLDepositorInsertGFP
UseTagsExpressionBacterialMutationPromoterPrpsLAvailable sinceMay 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBW2287_pCAG-PV1-PYL-L1-FlpO-397C-NLS-BGHpA
Plasmid#108770PurposeExpresses aa397 to C-terminus of Flp recombinase fused to PYL CIDDepositorInsertFlp recombinase fragment aa397 to C-terminus
UseCre/Lox and Synthetic BiologyTagsNLS and PYLExpressionMammalianMutationPromoterpCAGAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSIBR004
Plasmid#177667PurposeSIBR Intron 4 (Int4) interrupts the CDS of FnCas12aDepositorTypeEmpty backboneUseCRISPRTagsExpressionBacterialMutationPromoterlacUV5Available sinceJan. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDONR221_SLC25A38
Plasmid#132053PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectorsDepositorInsertSLC25A38 (SLC25A38 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 4, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
TFORF1513
Plasmid#143513PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertNKX3-1 (NKX3-1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJuly 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
BFP-Sec61 beta
Plasmid#49154Purposeexpression of Sec61 beta fused to BFP, used as a general ER markerDepositorInsertSec61 beta (SEC61B Human)
UseTagsBFPExpressionMammalianMutationPromoterCMVAvailable sinceMay 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDrVSP(C302S)-IRES2-EGFP
Plasmid#80331PurposeExpresses Danio rerio VSP (without phosphatase activity) with separated EGFP in mammalian cellDepositorInsertDaino rerio voltage-sensing phosphatase (tpte Zebrafish)
UseTagsExpressionMammalianMutationC302SPromoterCMVAvailable sinceSept. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCX-OKS-2A
Plasmid#19771PurposeNon-integrating (episomal) expression of mouse Oct3/4, Klf4 and Sox2DepositorUseTagsExpressionMammalianMutationThree genes are connected with the foot-and-mouth…PromoterAvailable sinceJan. 5, 2009AvailabilityAcademic Institutions and Nonprofits only -
pCMV-15F11-HA-Halo
Plasmid#129592PurposeThe encoded protein is the anti-HA scFv (anti-HA frankenbody) fused with the HaloTag. It can be used to track mature and nascent HA tagged proteins in living organism.DepositorInsertAnti-HA frankenbody-HaloTag (15F11-HA scFv-HaloTag)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRS316-4M5.3
Plasmid#45831DepositorInsert4M5.3
UseTagsFLAGExpressionYeastMutationPromoterGAL1Available sinceJune 26, 2013AvailabilityAcademic Institutions and Nonprofits only -
PB-TAC-OSKM
Plasmid#80481PurposepiggyBac transposon for dox-inducible expression of the OSKM polycistronic reprogramming cassette (with mCherry)DepositorUsePiggybac transposonTagsExpressionMammalianMutationPromotertetOAvailable sinceNov. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLOX-gfp-iresTK
Plasmid#12243DepositorInsertGFP, HSV1-TK
UseCre/Lox and LentiviralTagsExpressionMammalianMutationLoxP in 3' LTR.PromoterAvailable sinceAug. 4, 2006AvailabilityAcademic Institutions and Nonprofits only -
PB-H2A-mScarlet-2A-AKT_KTR-mTq2-2A-ERK_KTR-mNG
Plasmid#129632PurposeVisualization of Akt and ERK activityDepositorInsertPB-H2A-mScarlet-2A-AKT_KTR-mTq2-2A-ERK_KTR-mNG
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pENTR-c-Met
Plasmid#31786DepositorInsertMet (MET Human)
UseEntry vectorTagsExpressionMutationPromoterAvailable sinceOct. 21, 2011AvailabilityAcademic Institutions and Nonprofits only -
pSLQ1651-sgRNA(F+E)-sgGal4
Plasmid#100549PurposesgGal4-expressing vector modified from Addgene plasmid #51024DepositorInsertsgGal4
UseCRISPRTagssgRNAExpressionMammalianMutationPromoterU6Available sinceSept. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAVS1-P-CAG-mCh
Plasmid#80492Purposedonor vector for AAVS1 targeting (puromycin selection) and constitutive mCherry expressionDepositorInsertmCherry
UseDonor vector (human)TagsExpressionMammalianMutationPromoterCAGAvailable sinceNov. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCMV-MtAcKRS (human-opti)
Plasmid#164195PurposeTo express a Methanosarcina thermophila derived AcKRS in mammalian cells for genetic code expansionDepositorInsertMtAcKRS-478(human-opti)
UseSynthetic BiologyTagsExpressionMammalianMutationD76G; L325M; L329I; Y330F; L333A; C372FPromoterCMVAvailable sinceFeb. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
Cre-IRES-PuroR
Plasmid#30205DepositorInsertCre recombinase (cre Enterobacteria phage P1)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceApril 12, 2012AvailabilityAcademic Institutions and Nonprofits only -
pSNAPf-C1
Plasmid#58186DepositorTypeEmpty backboneUseTagsSNAP tagExpressionMammalianMutationPromoterAvailable sinceJan. 6, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
Sfp pet29b C-terminal His Tag
Plasmid#75015PurposeExpresses the PPTase Sfp from B. subtilis; loads CoA analogues onto ACPDepositorInsertSfp
UseTagsHis TagExpressionBacterialMutationPromoterT7Available sinceMay 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLDLR-Luc mutSRE
Plasmid#14945DepositorInsertLDLR promoter mut SRE (LDLR Human)
UseLuciferaseTagsluciferaseExpressionMammalianMutationPoint mutation in the SRE-1 sequence of the LDL r…PromoterAvailable sinceMay 21, 2007AvailabilityAcademic Institutions and Nonprofits only -
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pLenti-EML4-ALK variant 3a L1196M/G1202R
Plasmid#183831PurposeExpress EML4-ALK fusion variant 3a (E6a;A20) harboring ALK L1196M/G1202R mutation in mammalian cellsDepositorUseLentiviralTagsThe fusion protein of EML4 exon 1-6a and ALK exon…ExpressionMammalianMutationL1196M/G1202RPromoterCMVAvailable sinceJune 1, 2022AvailabilityAcademic Institutions and Nonprofits only