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Plasmid#120543PurposeExpresses dFP biliverdin-binding fluorescence-activating mini 10 KDa protein in bacteria.DepositorInsertde novo fluorescence-activating protein mini (dFP-mini)
UseTags6xHis - TEV cleavageExpressionBacterialMutationPromoterT7Available sinceJan. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-BLU-VIPR
Plasmid#220497PurposeRetroviral plasmid allowing for potent induction of gRNA using blue light. The plasmid contains VPR-EL222-2A-Thy1.1 and the C120 element followed by cloning sites for ribozyme flanked gRNA (RGR).DepositorTypeEmpty backboneUseCRISPR and RetroviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBLU-VIPR
Plasmid#220498PurposePlasmid allowing for potent induction of gRNA using blue light. The plasmid contains VPR-EL222 and the C120 response element followed by mCherry and cloning sites for ribozyme flanked gRNA (RGR).DepositorTypeEmpty backboneUseCRISPR; TransposonTagsExpressionMammalianMutationPromoterAvailable sinceAug. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAG-PDZD8HA
Plasmid#105005PurposeMammalian Expression of PDZD8DepositorInsertPDZD8 (Pdzd8 Mouse)
UseTagsHAExpressionMammalianMutationPromoterCAGAvailable sinceJan. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV Fcgamma RIIa IRES neo
Plasmid#14948DepositorInsertFcgamma RIIa (FCGR2A Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 21, 2007AvailabilityAcademic Institutions and Nonprofits only -
LentiV_Blast
Plasmid#111887PurposeLentiviral expression of cDNA with blasticidin resistance geneDepositorTypeEmpty backboneUseLentiviralTagsFLAGExpressionMammalianMutationPromoterEFS promoterAvailable sinceJune 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCRISPaint-T2A-Gal4-3xP3-RFP
Plasmid#127556PurposeCRISPaint universal donor plasmid for gene exon insertion. Encodes T2A-Gal4-SV40 and 3xP3-RFP visible eye marker.DepositorInsertGal4
UseCRISPRTagsExpressionInsectMutationPromoterAvailable sinceAug. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGM1190
Plasmid#69994PurposeOriT containing mobilizable vector for thiostrepton induced gene expression in StreptomycesDepositorInsertsaac
tsr
rep
sso
TipA promoter
dso
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFPV25.1_dGFPmut3-AAV
Plasmid#187377PurposerpsM promoter driving the expression of destabilized GFPmut3 (containing a AAV C-terminal tail)DepositorInsertdGFPmut3_AAV
UseTagsAAVExpressionBacterialMutationC-terminal AAV tailPromoterAvailable sinceAug. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
RhoC FLARE.sc mCer, mVenus - CA
Plasmid#65424PurposeRhoC CA biosensor single chain; mCerulean-tagged ROCK-RBD, mVenus-tagged RhoC Q63LDepositorInsertRBD, mCerulean, mVenus, RhoC
UseTagsExpressionBacterial, Insect, and Mamm…MutationT153M in mVenus, Q63L in RhoCPromoterAvailable sinceAug. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
RhoC FLARE.sc mCer, mVenus - DN
Plasmid#65423PurposeRhoC DN biosensor single chain; mCerulean-tagged ROCK-RBD, mVenus-tagged RhoC T19NDepositorInsertRBD, mCerulean, mVenus, RhoC
UseTagsExpressionBacterial, Insect, and Mamm…MutationT153M in mVenus, T19N in RhoCPromoterAvailable sinceAug. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
VGluT1-SNAPtag
Plasmid#107371PurposeSynaptic vesicle protein VGluT1 fused to the SNAPtag domainDepositorInsertVesicular Glutamate transporter 1 (Slc17a7 Rat)
UseTagsSNAPtag, 104 aa before C termExpressionMammalianMutationPromoterAvailable sinceSept. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9n(BB) (PX460)
Plasmid#48873PurposeCas9n (D10A nickase mutant) from S. pyogenesDepositorHas ServiceCloning Grade DNAInserthSpCas9n
UseCRISPRTags3XFLAGExpressionMammalianMutationCas9 D10A nickase mutantPromoterCbhAvailable sinceOct. 29, 2013AvailabilityAcademic Institutions and Nonprofits only -
GFP-TREX1(D18N)
Plasmid#27220DepositorInsertTREX1 (TREX1 Human)
UseTagsGFPExpressionMammalianMutationD18N sequence 52-GAC changed to AAC, resulting i…PromoterAvailable sinceJan. 26, 2011AvailabilityAcademic Institutions and Nonprofits only -
pPUR-hU6-sgRNA-Sirius-8XMS2
Plasmid#121942PurposeCRISPR-Sirius plasmidDepositorInsertsgRNA-Sirius-8XMS2
UseCRISPR and LentiviralTagsnoExpressionMammalianMutationPromoterhuman U6 promoterAvailable sinceFeb. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLenti6/UbC/mSlc7a1
Plasmid#17224DepositorInsertSlc7a1 (Slc7a1 Mouse)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 8, 2008AvailabilityAcademic Institutions and Nonprofits only -
pUBQ10-mNeonGreen-GW
Plasmid#209352PurposeGateway cloning compatible binary vector for agrobacterium mediated transformation. Arabidopsis UBQ10 promoter expression of N-terminal mNeonGreen fusion protein.DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceFeb. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDEST-HemmarR2
Plasmid#112813Purposegateway cloning destination vector for expressing enhancer driven CD4-tdTomatoDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJune 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEMHE-H2B-mEGFP
Plasmid#105528PurposeT7 promotor drives in vitro transcription of mEGFP-tagged Histon 2B mRNADepositorInsertH2B (H2BC11 Human)
UseIn vitro transcriptionTagsmEGFPExpressionMutationPromoterT7Available sinceApril 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDEST-HemmarG2
Plasmid#112814Purposegateway cloning destination vector for expressing enhancer driven CD4-tdGFPDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 10, 2018AvailabilityAcademic Institutions and Nonprofits only