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Plasmid#129508PurposeExpresses avGFP (Lambert 2019 clone) constitutively in E. coli (most strains)DepositorInsertavGFP
UseTagsExpressionBacterialMutationPromotersynthetic constitutive (stationary phase) promoteā¦Available sinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 mHIF-2Ī± MYC (P405A/P530V/N851A)
Plasmid#44027DepositorInsertmHIF-2Ī± (P405A/P530V/N851A) (Epas1 Mouse)
UseTagsMycExpressionMammalianMutationP405A, P530V, N851APromoterCMVAvailable sinceMarch 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAAV-GfaABC1D-GRAB_eCB2.0
Plasmid#164609PurposeExpress the endocannabinoid sensor GRAB_eCB2.0 in astrocytesDepositorInsertEndocannabinoid sensor GRAB_eCB2.0
UseAAVTagsExpressionMutationS383TPromoterGfaABC1DAvailable sinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
KAT6B-Tol2
Plasmid#113384Purposeevaluate FOXA1 DIV motif enhancer activity near 50 kb of KAT6B geneDepositorInsertKAT6B-enhancer (KAT6B Human)
UseTol2 reporterTagsExpressionMutationPromoterAvailable sinceSept. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
KAT6B-luciferase
Plasmid#113353Purposeevaluate FOXA1 DIV motif enhancer activity near 50 kb of KAT6B geneDepositorInsertKAT6B-enhancer (KAT6B Human)
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceSept. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
Integrin beta5-2XEGFP
Plasmid#139779PurposeDetecting Integrin beta5 by fluorescence microscopyDepositorInsertIntegrin subunit beta5 (ITGB5 Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceApril 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pODN-mNG-RHOA
Plasmid#183836PurposeRepair template for the N-terminal tagging of RhoA with mNeonGreen in human cells using CRISPR/Cas9.DepositorInsertRHOA homology arms with mNeonGreen-linker (RHOA Human)
UseCRISPR; Donor templateTagsmNeonGreen-linkerExpressionMammalianMutationHomology arms contain point mutations to remove tā¦PromoterAvailable sinceMay 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-eGFP-Ago2
Plasmid#74231PurposeGFP-Ago2 lentiviral vectorDepositorInsertAgo2 (AGO2 Human)
UseLentiviralTagseGFPExpressionMutationPromoterAvailable sinceApril 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CMV-BDNF-Myc
Plasmid#124279PurposeExpress Myc-tagged brain-derived neurotrophic factor (BDNF)DepositorInsertBDNF (Bdnf Mouse)
UseAAVTagsMycExpressionMutationPromoterAvailable sinceApril 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCol1a1-frt (FVB)
Plasmid#63575PurposeTargeting vector for the Col1a1 locus that contains a Neo resistance cassette flanked by FRT sites, followed by an ATG-less Hygromycine resistance gene. The homology arms match the sequence of FVB.DepositorInsertCol1A-frt-hygro-pA
UseMouse Targeting; Frt/flpeTagsExpressionBacterial and MammalianMutationPromoterNoneAvailable sinceMarch 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTet-GLI2shR
Plasmid#136691PurposeInducible Gli2 shRNA lentiviral plasmid (target sequence: human GLI2 5ā²CCGGCCTGGCATGACTACCACTATGCTCGAGCATAGTGGTAGTCATGCCAGGTTTTTG 3ā²)DepositorInsertshRNA_humanGli2 (GLI2 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJune 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pZac2.1 SV40-CMV-SaCas9-3xNLS
Plasmid#78601PurposeAAV vector containing SaCas9DepositorInsertSV40-CMV-SaCas9-3xNLS
UseAAV and CRISPRTagsHA tagExpressionMammalianMutationPromoterSV40, CMV promotersAvailable sinceDec. 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCS2-MT mouse Axin (Axin MTFu1)
Plasmid#21287DepositorInsertAxin (Axin1 Mouse)
UseTagsMycExpressionMammalianMutationPromoterAvailable sinceDec. 15, 2009AvailabilityAcademic Institutions and Nonprofits only -
FH1-PNGT#1-mCherry_PGK-H2B-CFP
Plasmid#164103PurposeFluorescent reporter for genetic tracing of proneural Glioblastoma cell-state.DepositorInsertPNGT#1-mCherry
UseLentiviral and Synthetic BiologyTagsExpressionMutationPromoterAvailable sinceMarch 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pN1-RnKIF5C(1-560)-Halo-Flag
Plasmid#188071PurposeMammalian cell expression of truncated version (aa 1-560) of rat kinesin-1 KIF5C tagged with Halo and Flag tagsDepositorInsertrat KIF5C (Kif5c Rat)
UseTagsFlag and HaloExpressionMammalianMutationaa1-560PromoterCMVAvailable sinceAug. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
H2B-mAvicFP1
Plasmid#129511PurposeExpresses H2B-mAvicFP1 in mammalian cells (human histone 2B)DepositorInsertH2B-mAvicFP1
UseTagsExpressionMammalianMutationmAvicFP1 fused to the C terminus of human histoneā¦PromoterCMVAvailable sinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pWPI_SPbFGF
Plasmid#25812DepositorInsertpWPI_SPbFGF (FGF2 Human)
UseLentiviralTagsExpressionMutationIg signal peptide in N-terminal of human FGF2PromoterAvailable sinceJan. 24, 2011AvailabilityAcademic Institutions and Nonprofits only -
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CDK4-3XFlag-pBabe
Plasmid#73558Purposevector encoding a fusion protein between CDK4 and 3 Flag tagsDepositorInsertCDK4 (CDK4 Human)
UseRetroviralTags3X-FLAGExpressionMammalianMutationPromoterSV40Available sinceAug. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGEX-UAP56
Plasmid#157661PurposeExpresses UAP56 in E.coli cellDepositorInsertSpliceosome RNA helicase DDX39B (DDX39B Human)
UseTagsGST tagExpressionBacterialMutationdeleted amino acids 1-43PromoterAvailable sinceAug. 20, 2020AvailabilityAcademic Institutions and Nonprofits only