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Plasmid#87956PurposeExpresses human gamma-tubulin with a GFP-tag on the C-terminal of the proteinDepositorInsertgamma-tubulin 1 (TUBG1 Human)
UseTagsGFPExpressionMammalianMutationThe protein is a sh-gamma-tubulin resistant gene …PromoterAvailable sinceFeb. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1.Gαs-LgB113
Plasmid#134362PurposeNanoluc complementation assay. Expression of Gαs protein fused with the LgBiT(LgB)of the nanoluciferase inserted between residues 113 and 114 of Gαs. Addition of the HA epitope at N terminus of Gαs.DepositorInsertGαs-LgB113 (GNAS Human)
UseTagsHAExpressionBacterial and MammalianMutationPromoterT7Available sinceJuly 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHR-Hsp-eGFP-PGK-mCherry
Plasmid#191862PurposeHeat inducible eGFP reporter with constitutive mCherry markerDepositorInsertseGFP
mCherry
UseLentiviralTagsExpressionMammalianMutationPromoterHSPA7 promoter and PGK promoterAvailable sinceNov. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-myc-EphB2
Plasmid#200982PurposeMammalian expression plasmid for myc-tagged EphB2DepositorInsertEphB2 (EPHB2 Human)
UseTagsExtracellular c-myc epitope tag and Signal peptid…ExpressionMammalianMutationPromoterCMVAvailable sinceOct. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
All-in-one lenti-evoCDA-NG
Plasmid#200449PurposeLentivirus vector for base editingDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJune 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 mHIF-1α MYC (P402A/P577A/N813A)
Plasmid#44028DepositorInsertmHIF-1α (P402A/P577A/N813A) (Hif1a Mouse)
UseTagsMycExpressionMammalianMutationP402A, P577A, N813APromoterCMVAvailable sinceMarch 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
Lamp1-YFP
Plasmid#1816PurposeMammalian expression of Lamp1 fused to YFP. Used for localization to the lysosome.DepositorInsertlysosome associated membrane protein 1 (Lamp1 Rat)
UseTagsYFPExpressionMammalianMutationD50E (not important for function of plasmid)PromoterAvailable sinceFeb. 16, 2006AvailabilityAcademic Institutions and Nonprofits only -
CDK4-3XFlag-pBabe
Plasmid#73558Purposevector encoding a fusion protein between CDK4 and 3 Flag tagsDepositorInsertCDK4 (CDK4 Human)
UseRetroviralTags3X-FLAGExpressionMammalianMutationPromoterSV40Available sinceAug. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pNCST-AausFP1
Plasmid#129499PurposeExpresses AausFP1 constitutively in E. coli (most strains)DepositorInsertAausFP1
UseTagsExpressionBacterialMutationPromotersynthetic constitutive (stationary phase) promote…Available sinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hsyn-GRAB_rDA1h
Plasmid#140557PurposeExpresses the red fluorescent high-affinity DA sensor GRAB_rDA1h in neuronsDepositorHas ServiceAAV9InsertRed fluorescent DA sensor GRAB_rDA1h (high affinity)
UseAAVTagsExpressionMutationPromoterhSynAvailable sinceJuly 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLenti-Puro-sgSNRK
Plasmid#138694PurposeExpresses a human SNRK-targeting sgRNA and Cas9DepositorInsertsgSNRK human (SNRK Human)
UseLentiviralTagsExpressionMutationPromoterhU6Available sinceFeb. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV-dCas9-D3A
Plasmid#78256PurposeExpresses dead Cas9 (dCas9) fused to DNMT3A catalytic domain (CD) under the control of the CMV promoterDepositorInsertDNMT3A CD aa 598-912 (DNMT3A Human)
UseCRISPRTagsFlag epitope tagExpressionMammalianMutationPromoterpCMVAvailable sinceJune 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CMV-BDNF-Myc
Plasmid#124279PurposeExpress Myc-tagged brain-derived neurotrophic factor (BDNF)DepositorInsertBDNF (Bdnf Mouse)
UseAAVTagsMycExpressionMutationPromoterAvailable sinceApril 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET41b+_Ufd1-His
Plasmid#117107PurposeProduction of recombinant Ufd1-His in E.coliDepositorInsertUfd1 (UFD1 Human)
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceOct. 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET41b+_Npl4
Plasmid#117108PurposeProduction of recombinant Npl4 in E.coliDepositorInsertNpl4 (NPLOC4 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMSCVpuro-Flag-cMyc T58A
Plasmid#20076DepositorInsertcMyc (MYC Human)
UseRetroviralTagsFlagExpressionMammalianMutationT58APromoterAvailable sinceJan. 9, 2009AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 mHIF-2α MYC (P405A/P530V/N851A)
Plasmid#44027DepositorInsertmHIF-2α (P405A/P530V/N851A) (Epas1 Mouse)
UseTagsMycExpressionMammalianMutationP405A, P530V, N851APromoterCMVAvailable sinceMarch 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
pIRES-hrGFP II-TET1_CD
Plasmid#83570PurposeExpresses the catalytic domain of TET1 in mammalian cellsDepositorInsertTet Methylcytosine Dioxygenase 1 (TET1 Human)
UseTags3X FLAG tag and hr GFP IIExpressionMammalianMutationTET1 amino acids 1-1417 deletedPromoterCMVAvailable sinceAug. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
FpG5
Plasmid#69443Purposelentiviral GFP reporter plasmid for high throughput functional detection of active transcriptional regulatory DNAsDepositorInsertsminimal FGF4 promoter
EGFP
Ubiquitin promoter
Hygromycin resistance
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMay 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLV_TRET_hNgn2_UBC_Puro
Plasmid#61474Purpose3rd generation lentiviral vector; TetON promoter driving human Ngn2; constitutive expression of Puromycin selection markerDepositorInsertsUseLentiviralTagsExpressionMutationPromoterAvailable sinceFeb. 2, 2015AvailabilityAcademic Institutions and Nonprofits only