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Plasmid#80404Purposeoverexpression of ISG15DepositorInsertISG15 (ISG15 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
Flag-Vac 14 Full Length
Plasmid#47418DepositorInsertVac14 (VAC14 Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceJuly 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
IFNAR1_pCSdest
Plasmid#53881DepositorInsertIFNAR1 (IFNAR1 Human)
UseTagsExpressionMammalianMutationPromoterSP6 and CMVAvailable sinceMarch 27, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
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Flag-SRm160
Plasmid#11305DepositorInsertSRm160 (SRRM1 Human)
UseTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceApril 4, 2006AvailabilityAcademic Institutions and Nonprofits only -
pLKO-RB1-shRNA19
Plasmid#25640Purpose3rd generation lentiviral vectorDepositorInsertRB1-shRNA 19 (RB1 Human)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceJune 21, 2010AvailabilityAcademic Institutions and Nonprofits only -
pCAG-EGxxFP
Plasmid#50716Purpose5’ and 3’ EGFP fragments that shares 482bp were placed under ubiquitous CAG promoter. Used for validation of gRNA sequences by DSB mediated EGFP reconstitution.DepositorInsertsplit EGFP
UseCRISPRTagsExpressionMammalianMutationPromoterCAGAvailable sinceFeb. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-PAK6
Plasmid#23833DepositorInsertPAK6 (PAK6 Human)
UseGateway donor vectorTagsExpressionMutationPromoterAvailable sinceJuly 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
pUBC_HA_PP7-mVenus
Plasmid#111268PurposeTransient expression of non-aggregating delta-FG mutant, fused to mVenus in mammalian cells, under a hUBC promoter, HA tagDepositorInsertPP7-mVenus
UseSynthetic BiologyTagsHA, SV40 NLS, and mVenusExpressionMammalianMutationPP7: C68S, S69G [delta]70-75 (non-aggregating)PromoterhUBCAvailable sinceJune 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBSKΔBS-SD_MCS_SA(NBSI)-24xMS2-sirius-96xopto-TetO-Int-3xFLAG-NeoR-GS4
Plasmid#177265PurposeSTREAMING-tag cassetteDepositorInsertSTREAMING-tag cassette
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceDec. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUbC-OsTIR1-myc-IRES-scFv-sfGFP
Plasmid#84563PurposeBicistronic vector for expression of O. sativa E3 ubiquitin ligase and single chain variable fragment of GCN4 antibody fused to superfolder GFPDepositorInsertTransport inhibitor response 1 (E3 ligase domain), IRES, single chain variable fragment-superfolder GFP
UseLentiviralTagsmyc tag (9x)ExpressionMutationPromoterAvailable sinceDec. 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
P362-FBP_12
Plasmid#139703PurposeT-DNA encoding Fungal Bioluminescent Pathway Components, complete pathwayDepositorInsertpSlH4:NnCPH:tSlH4, p35S_Short_TMV 5':AsNpga:tAtug7, pAtuMas:NhH3H:tAtuMas, p35S:NnHisps:t35S, p35S:NnLuz:AtuOcs
UseLuciferaseTagsExpressionPlantMutationPromotervariousAvailable sinceMay 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
PresentER-SIINFEKL (mCherry)
Plasmid#102945PurposePresentER minigene encoding H2-Kb ligand SIINFEKL (mCherry)DepositorInsertSIINFEKL (OVAL Synthetic, Chicken)
UseRetroviralTagsExpressionMammalianMutationOVA257-264PromoterLTRAvailable sinceApril 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
TFORF3467
Plasmid#144943PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertSUPT4H1 (SUPT4H1 Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterEF1aAvailable sinceAug. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
FUGW-ER-TurboID
Plasmid#200965Purpose3rd gen lentiviral plasmid for expressing endoplasmic reticulum localized TurboID with EGFPDepositorInsertER-TurboID
UseLentiviralTagsEGFPExpressionMammalianMutationPromoterAvailable sinceAug. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
Proinsulin-NanoLuc in pLX304
Plasmid#62057PurposeLuminescent reporter of insulin secretionDepositorInsertProinsulin-NanoLuc (Ins2 Synthetic, Mouse)
UseLentiviral and LuciferaseTagsExpressionMammalianMutationInserted NanoLuc into C-peptide of mouse Ins2 cod…PromoterCMVAvailable sinceJan. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSIR-GFP
Plasmid#51134PurposeSelf-inactivating retrovirus vector containing the GFP geneDepositorTypeEmpty backboneUseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
TFORF3175
Plasmid#144651PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertILF3 (ILF3 Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET32a-HD46Q
Plasmid#11515DepositorInserthuntingtin, exon 1, 46Q glutamines (HTT Human)
UseTagsHis, His (out of frame), and TrxExpressionBacterialMutationhuntingtin exon 1 with 46 glutamines, vector m…PromoterAvailable sinceApril 28, 2006AvailabilityAcademic Institutions and Nonprofits only -
pDRF1-GW ytdTomato
Plasmid#118456PurposeOverexpression of yeast codon-optimized tdTomatoDepositorInsertytdTomato
UseTagsExpressionYeastMutationPromoterAvailable sinceFeb. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCTCON2-BYG
Plasmid#158126PurposeDual fluorescent protein reporter (BFP-GFP) for ncAA incorporation (wild-type construct)DepositorInsertmTagBFP
UseTagsExpressionYeastMutationPromoterGAL1-10Available sinceJan. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
TRMPVneo.Yap.3093
Plasmid#59915PurposeTet on shRNA for conditional Yap1 knockdownDepositorInsertYap1 (Yap1 Mouse)
UseRetroviralTagsExpressionMutationPromoterAvailable sinceSept. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
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rescue plasmid
Plasmid#135971Purposeexpresses copGFP and puromycin resistance genesDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceMay 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
R5 EMCV
Plasmid#51733PurposeDicistronic vector with EMCV IRESDepositorInsertEMCV IRES
UseTagsExpressionMutationPromoterAvailable sinceJune 12, 2014AvailabilityAcademic Institutions and Nonprofits only -
TRE-UBC-mCD47-T2A-GFP
Plasmid#205449Purposelentiviral plasmid for expression of mouse CD47DepositorInsertmCD47 (Cd47 Mouse)
UseTagsExpressionMammalianMutationPromoterUBCAvailable sinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pU6_(GLuc)_INT[3xPP7_SL]
Plasmid#68424PurposeTransient expression of an "INT" construct_bearing three PP7 Stem-loops, targeting the GLuc reporter, in mammalian cells. U6 promoterDepositorInsertINT construct bearing three PP7 Stem-loops
UseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterhuman U6Available sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEF1a_FL MUT TET1
Plasmid#124081PurposeExpression of catalytic inactive form of full-length TET1DepositorInsertTET1 - full length, mutated (TET1 Human)
UseTagsFlag and HAExpressionMammalianMutationH1672Y, D1674APromoterEF1 alphaAvailable sinceApril 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
Integrin β3-GFP
Plasmid#205091PurposeExpress GFP-tagged human integrin β3 in mammalian cellsDepositorInsertIntegrin beta 3 (ITGB3 Human)
UseTagsGFPExpressionMammalianMutationPromoterAvailable sinceSept. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pROP-PP-FBFP
Plasmid#122137PurposeFMN-based fluorescent protein Reporter plasmid for B-Rex contain perfect operator sequence (anaerobic purpose)DepositorInsertFBFP
UseTagsExpressionBacterialMutationPromoterJ23101Available sinceMarch 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
mCardinal-pBAD
Plasmid#54800PurposeLocalization: Protein Expression Vector , Excitation: 604, Emission: 659DepositorTypeEmpty backboneUseTags6xHis and mCardinalExpressionBacterialMutationPromoterAvailable sinceJuly 25, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pJL 89
Plasmid#98558PurposeUseful for making infectious clones of RNA virus cDNAs; Has 35S promoter and HDV Ribozyme sequences in T-DNA region of Agrobacterium vector.DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterEnh 35SAvailable sinceAug. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
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pVS1
Plasmid#33143DepositorInsertLacO (256 copies)
UseTagsExpressionYeastMutationPromoterAvailable sinceDec. 20, 2011AvailabilityAcademic Institutions and Nonprofits only -
pET24_NusA-Tus
Plasmid#165959PurposeExpression of soluble E. coli Tus proteinDepositorInsertTus
UseTagsHis-NusA-TevExpressionBacterialMutationPromoterT7Available sinceAug. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEGFP VAMP8
Plasmid#42311DepositorInsertVAMP 8 (VAMP8 Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceMarch 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
pEGFP VAMP4
Plasmid#42313DepositorInsertVAMP 4 (VAMP4 Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceMarch 1, 2013AvailabilityAcademic Institutions and Nonprofits only -
v3em-Cterm-PE6c-dualU6-Rosa26-twinPE-attB
Plasmid#207861PurposeAAV genome encoding C-terminal PE6c and U6 expression cassettes for Rosa26 twinPE pegRNA pairsDepositorInsertNpuC-Cterm-PE6c-dualU6-Rosa26-twinPE-attB
UseAAVTagsExpressionMutationSee ManuscriptPromoterCbhAvailable sinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
miniCGBE1 (pBM1219)
Plasmid#140253PurposeCMV promoter expression plasmid for rAPOBEC1(R33A)-nCas9-P2A-EGFPDepositorInsertBE4max(R33A)-ΔUGI
UseTagsExpressionMammalianMutationR33A in rAPOBEC1, D10A in Cas9PromoterCMVAvailable sinceAug. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
TFORF0113
Plasmid#141520PurposeLentiviral vector for overexpressing the SMAD3 transcription factor ORF with a unique 24-bp barcode. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertSMAD3 (SMAD3 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJAT31
Plasmid#204291PurposeFor inserting a phiC31 attP site. HDR integrant is marked with ie1-DsRed, which is expressed in abdomen and mouthparts. Second U6 promoter allows use of two different gRNAs in two synthesized arms.DepositorInsertie1-DsRed
UseCRISPRTagsExpressionMutationPromoterie1Available sinceSept. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAC1816_pCR8-gSMN2-DN4
Plasmid#118649PurposeTransient transfection; Expressed SMN2-DN4 CasRx gRNA; Gateway DonorDepositorInsertgSMN2-DN4
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceJan. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV-HA-PD-L1
Plasmid#121492PurposeExpression of human PD-L1 WT in mammalian cellsDepositorInsertPD-L1 (CD274 Human)
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
CDK7 gRNA (BRDN0001162216)
Plasmid#76077Purpose3rd generation lentiviral gRNA plasmid targeting human CDK7DepositorInsertCDK7 (CDK7 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
TFORF2026
Plasmid#144326PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertNPAS3 (NPAS3 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceAug. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF1958
Plasmid#144118PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertCUX1 (CUX1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLIX403-ccdB-G418
Plasmid#158561PurposeSet of empty Gateway Cloning compatible, inducible, lentiviral vectors with various mammalian selection markers and N-terminal fluorescent protein fusionsDepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
EF1α-scFv-p65-HSF1-T2A-EGFP-WPRE-PolyA
Plasmid#107311PurposeEncoding scFv-p65-HSF1 driven by EF1α promoterDepositorInsertEF1α-scFv-p65-HSF1-T2A-EGFP-WPRE-PolyA
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
RGB-S reporter
Plasmid#207841PurposeA three-colour stress biosensor for real time analysis of physiological stress, genotoxicity, and cytotoxicity of Escherichia coliDepositorInsertsRpoH sensing construct
SOS sensing construct
RpoS sensing construct
UseTagsExpressionBacterialMutationPromoterPosmY, PsulA, and grpEAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMitoLOC
Plasmid#58980PurposeExpression of preCOX4-mCherry & preSU9-GFP for colocalisation analysis of yeast mitochondrilaDepositorInsertsmCherry
GFP
UseTagspreCOX4 and preSU9ExpressionYeastMutationPromoterADH1 and MET17Available sinceJuly 7, 2015AvailabilityAcademic Institutions and Nonprofits only