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Plasmid#98877PurposeIn vivo visualization of actin cytoskeleton (can be used for colocalization studies)DepositorArticleInsertLifeAct
UseTagsmNeonGreenExpressionMammalianMutationPromoterCMVAvailable sinceMarch 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
HA-EglN1-pcDNA3
Plasmid#18963DepositorInsertEgg laying Nine - 1 (EGLN1 Human)
UseTagsHA-tagExpressionMammalianMutationwt cDNAPromoterAvailable sinceAug. 20, 2008AvailabilityAcademic Institutions and Nonprofits only -
FUW-tetO-hOKMS
Plasmid#51543PurposeInducible expression of polycistronic 2A spaced OCT4-KLF4-MYC-SOX2DepositorUseLentiviralTagsExpressionMammalianMutationPromotertetOAvailable sinceMay 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCMV-ABE7.9
Plasmid#102918Purposeexpresses ABE7.9 in mammalian cellsDepositorInsertABE7.9
UseTagsNLSExpressionMammalianMutationsee manuscriptPromoterCMVAvailable sinceNov. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFRT_TO_DESTFLAGHA
Plasmid#26361DepositorTypeEmpty backboneUseTagsFLAG/HAExpressionMammalianMutationPromoterAvailable sinceSept. 22, 2010AvailabilityAcademic Institutions and Nonprofits only -
pLenti-MP2
Plasmid#36097Purpose3rd generation lentiviral plasmidDepositorTypeEmpty backboneUseLentiviralTagsExpressionMutationPromoterAvailable sinceJune 1, 2012AvailabilityAcademic Institutions and Nonprofits only -
paavCAG-iCre
Plasmid#51904PurposeCre recombinaseDepositorInsertimproved Cre recombinase
UseAAVTagsExpressionMutationPromoterCAGAvailable sinceApril 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
CMV-R-GECO1
Plasmid#32444PurposeMammalian expression of Red fluorescent genetically encoded Ca2+ indicator for optical imagingDepositorArticleInsertR-GECO1.0
UseTagsExpressionMammalianMutationSubstitutions relative to the mApple-derived anal…PromoterCMVAvailable sinceSept. 29, 2011AvailabilityAcademic Institutions and Nonprofits only -
pAct:dCas9-VPR
Plasmid#78898PurposeExpresses dCas9-VPR under Actin promoter for CRISPRa in Drosophila cell culture. Please note- this plasmid does not express GFP.DepositorInsertdCas9-VPR
UseCRISPRTagsExpressionInsectMutationPromoterpActin (Drosophila)Available sinceAug. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET15b PKA Cat
Plasmid#14921DepositorInsertPKA catalytic subunit alpha (Prkaca Mouse)
UseTags6xHis and Thrombin cleavage siteExpressionBacterialMutationPromoterAvailable sinceJuly 24, 2007AvailabilityAcademic Institutions and Nonprofits only -
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IKK-2 K44M
Plasmid#11104DepositorInsertIKK-2 (IKBKB Human)
UseTagsFLAGExpressionMammalianMutationK44M, kinase inactivePromoterAvailable sinceFeb. 14, 2006AvailabilityAcademic Institutions and Nonprofits only -
HA-BirA*-pDEST-N-pcDNA5/FRT/TO
Plasmid#118375PurposeExpression vector for BioID in mammalian system. Gateway compatible destination vector to generate stable, inducible cell line expressing N-terminally tagged HABirA* and protein of interest.DepositorTypeEmpty backboneUseDestinationTagsHA-BirA*ExpressionMammalianMutationPromoterAvailable sinceNov. 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.3_SARS2_XBB.1.5
Plasmid#196585Purposeexpressing SARS-CoV-2 XBB.1.5 spike protein for pseudovirus productionDepositorInsertSpike (XBB.1.5)
UseTagsExpressionMammalianMutationG252V, F486P on the basis of XBBPromoterCMVAvailable sinceMarch 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-H2B-GFP
Plasmid#116869PurposeNuclear localized GFPDepositorInsertGFP
UseAAVTagsExpressionMutationPromoterAvailable sinceNov. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
DR274
Plasmid#42250Purposeguide RNA (gRNA) expression vector used to create a gRNA to a specific sequence; uses T7 promoter for in vitro transcriptionDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseCRISPR; Zebrafish expressionTagsExpressionWormMutationPromoterT7Available sinceJan. 31, 2013AvailabilityAcademic Institutions and Nonprofits only -
pECE-HA-AMPKbeta1 wt
Plasmid#31666DepositorInsertprotein kinase, AMP-activated, beta 1 non-catalytic subunit (PRKAB1 Human)
UseTagsHA (YDVPDYASL)ExpressionMammalianMutationPromoterAvailable sinceApril 11, 2012AvailabilityAcademic Institutions and Nonprofits only -
CMV-B-GECO1
Plasmid#32448PurposeMammalian expression of blue fluorescent genetically encoded Ca2+ indicator for optical imagingDepositorArticleInsertB-GECO1.0
UseTagsExpressionMammalianMutationGCaMP3 S40P/L60P/D86G/K119I/L173Q/T223S/Y224H/D30…PromoterCMVAvailable sinceSept. 30, 2011AvailabilityAcademic Institutions and Nonprofits only -
pAAV.cTNT.iCre
Plasmid#69916PurposeUsed to package AAV9:cTNT.iCre, which specifically transduce cardiomyocytes and express Cre recombinase. Only works in vivo.DepositorInsertimproved cre (iCre) recombinase and tdTomato
UseAAVTagsThe iCre coding frame and tdTomato coding frame i…ExpressionMutationPromoterChicken cardiac troponin TAvailable sinceDec. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pdCas9-DNMT3A-EGFP
Plasmid#71666PurposeExpression of human codon-optimized dCas9-DNMT3A fusion with T2A-EGFP for targeted DNA methylation in mammalian cells; cloning backbone for sgRNADepositorInsertS. pyogenes dCas9 fused with the catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-EGFP (DNMT3A Human, S. pyogenes, Synthetic)
UseCRISPRTags3xFLAG, SV40 NLS, and T2A-EGFPExpressionMammalianMutationD10A and H840A in S.pyogenes Cas9PromoterCBhAvailable sinceMarch 7, 2016AvailabilityAcademic Institutions and Nonprofits only