Addgene

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pUCBB-oriT

Plasmid

#32556

Depositor

Claudia Schmidt-dannert

Article

Vick et al Appl Microbiol Biotechnol. 2011 Dec;92(6

Insert

oriT

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

N/A

Available since

Oct. 18, 2011

Availability

Academic Institutions and Nonprofits only

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pTR47m4

Plasmid

#104068

Purpose

Luciferase Reporter for Formaldehyde Sensing

Depositor

David Liu

Article

Woolston et al Biotechnol Bioeng. 2017 Sep 16. doi: 10.

Inserts

xluxAB
frmR
frm Promoter m4

Use

Luciferase and Synthetic Biology

Tags

Expression

Bacterial

Mutation

23r and 25th nucleotides in sequence mutated from…

Promoter

Available since

March 7, 2018

Availability

Academic Institutions and Nonprofits only

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pFastBacDual with PA+Histag

Plasmid

#84870

Purpose

Baculovirus Expression of Bacillus anthracis protective antigen

Depositor

Russell Vance

Article

Rauch et al J Exp Med. 2016 May 2;213(5):657-65. doi

Insert

PA

Use

Baculovirus

Tags

His

Expression

Insect

Mutation

Promoter

Polyhedrin

Available since

Nov. 14, 2016

Availability

Academic Institutions and Nonprofits only

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AAV-EF1a-BbTagBY

Plasmid

#45185

Depositor

Dawen Cai, Joshua Sanes

Article

Cai et al Nat Methods. 2013 May 5;10(6):540-7. doi

Has Service

AAV9

Insert

pAAV-EF1a-Brainbow-invert tagBFP-EYFP-WPRE

Use

AAV; Adeno-associated viral

Tags

Expression

Mammalian

Mutation

Promoter

human EF1a

Available since

Nov. 14, 2013

Availability

Academic Institutions and Nonprofits only

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AAV-EF1a-BbChT

Plasmid

#45186

Depositor

Dawen Cai, Joshua Sanes

Article

Cai et al Nat Methods. 2013 May 5;10(6):540-7. doi

Has Service

AAV9

Insert

pAAV-Ef1a-Brainbow/mCherry/mTFP-WPRE

Use

AAV; Adeno-associated viral

Tags

Expression

Mammalian

Mutation

Promoter

human EF1a

Available since

Nov. 14, 2013

Availability

Academic Institutions and Nonprofits only

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pET19b+H3

Plasmid

#157807

Purpose

Expression of cysteine-free human histone H3 (c111A)

Depositor

Michael Rosen

Article

Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

Insert

H3

Use

Tags

Expression

Bacterial

Mutation

C111A

Promoter

T7

Available since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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Thy1 promoter construct

Plasmid

#20736

Depositor

Joshua Sanes

Article

Feng et al Neuron. 2000 Oct . 28(1):41-51.

Insert

Thy1 promoter (Thy1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 14, 2009

Availability

Academic Institutions and Nonprofits only

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IL-2 promoter luciferase

Plasmid

#12194

Depositor

Anjana Rao

Article

Macian et al EMBO J. 2000 Sep 1. 19(17):4783-95.

Insert

IL-2 promoter (Il2 Mouse)

Use

Luciferase

Tags

luciferase

Expression

Bacterial

Mutation

Promoter

Available since

July 20, 2006

Availability

Academic Institutions and Nonprofits only

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pIOM15

Plasmid

#20869

Depositor

Carmen Beuzón

Article

Ortiz-Martin et al J Microbiol Methods. 2006 Dec . 67(3):39

Insert

aphA::aadA

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 15, 2009

Availability

Academic Institutions and Nonprofits only
mWasabi-C1

Plasmid

#54743

Purpose

Localization: C1 Cloning Vector, Excitation: 493, Emission: 509

Depositor

Michael Davidson, Robert Campbell

Article

Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

Type

Empty backbone

Use

Tags

mWasabi

Expression

Mammalian

Mutation

Promoter

Available since

June 11, 2014

Availability

Academic Institutions and Nonprofits only

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pEGFP-MRLC1

Plasmid

#35680

Depositor

Tom Egelhoff

Article

Beach et al BMC Cell Biol. 2011 Dec 2;12:52.

Insert

MRLC1 (MYL9 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 24, 2012

Availability

Academic Institutions and Nonprofits only
pRF TpC hTERT intron-1

Plasmid

#171070

Purpose

Firefly-Renilla luciferase assay for hTERT promoter activity of hTERT intron-1

Depositor

Agnel Sfeir

Article

Penev et al Mol Cell. 2021 Apr 6. pii: S1097-2765(21

Insert

hTERT intron-1

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pKL10-mRuby-iATPSnFR1.0 bb118

Plasmid

#158980

Purpose

Constitutive expression (sigma 70 promoter) of the ratiometric mRuby-iATPSnFR1.0 ATP indicator in E. coli.

Depositor

Joel Kralj

Article

Bruni et al Elife. 2020 Aug 4;9. pii: 58706. doi: 10

Insert

mruby-iATPSNfR1.0

Use

Tags

mruby3

Expression

Bacterial

Mutation

codon optimized for e. coli from Addgene#102557

Promoter

Bba_J23118 (sigma 70)

Available since

Sept. 18, 2020

Availability

Academic Institutions and Nonprofits only

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CMV-Mito4x-jRCaMP1b

Plasmid

#127873

Purpose

Red-shifted fluorescent reporter for mitochondrial matrix Ca2+

Depositor

Timothy Ryan

Article

Ashrafi et al Neuron. 2019 Dec 10. pii: S0896-6273(19)

Insert

Red-shifted mitochondrial matrix Ca2+ indicator

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3 Plk4(Sak) wt (Nigg HR9)

Plasmid

#41165

Depositor

Erich Nigg

Article

Habedanck et al Nat Cell Biol. 2005 Nov;7(11):1140-6.

Insert

Plk4 (PLK4 Human)

Use

Tags

3xMyc and PreScission Site

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 7, 2013

Availability

Academic Institutions and Nonprofits only

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GfaABC1D-pm-iATPSnFR1.0

Plasmid

#102552

Purpose

expresses membrane displayed ATP sensor in astrocytes

Depositor

Baljit Khakh

Article

Lobas et al Nat Commun. 2019 Feb 12;10(1):711. doi:

Insert

iATPSnFR1.0

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

Jan. 10, 2019

Availability

Academic Institutions and Nonprofits only

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pCAG-NL1AB

Plasmid

#15262

Depositor

Peter Scheiffele

Article

Chih et al Neuron. 2006 Jul 20. 51(2):171-8.

Insert

Neuroligin 1 with inserts A and B (Nlgn1 Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 23, 2009

Availability

Academic Institutions and Nonprofits only

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pLVX-mCherry

Plasmid

#180646

Purpose

Lentiviral expression vector for mCherry. Dox-inducible. Internal ID: WISP20-61.

Depositor

Wesley Sundquist

Article

Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

Insert

mCherry

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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FlucSM-HA-GFP11-N1

Plasmid

#91955

Purpose

Expresses aggregation-prone FlucSM-HA-GFP11 in mammalian cells

Depositor

Rong Li

Article

Ruan et al Nature. 2017 Mar 16;543(7645):443-446. d

Insert

FlucSM-HA-GFP11

Use

Tags

Expression

Mammalian

Mutation

R188Q

Promoter

CMV

Available since

June 6, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Flag-Tipin

Plasmid

#22889

Depositor

Aziz Sancar

Article

Unsal-Kacmaz et al Mol Cell Biol. 2007 Apr . 27(8):3131-42.

Insert

Tipin (TIPIN Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 28, 2010

Availability

Academic Institutions and Nonprofits only