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18,075 results

Showing: 4061 - 4080 of 18075 results

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pAC-VIOL

Plasmid

#53087

Purpose

Produces the carotenoid violaxanthin in E. coli.

Depositor

Francis X Cunningham Jr

Article

Neuman et al Plant J. 2014 Apr;78(1):80-93. doi: 10.1

Insert

zep (ABA1 Mustard Weed)

Use

Low copy number bacterial cloning vector

Tags

Expression

Mutation

Lacks codons for the first 60 N-terminal amino ac…

Promoter

T7

Availability

Academic Institutions and Nonprofits only

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pET15b-AavLEA1

Plasmid

#53093

Purpose

AavLEA1 expression (in BL21 DE3 E. coli strain)

Depositor

Claude Férec

Article

Tripathi et al PLoS One. 2014 Aug 13;9(8):e104970. doi:

Insert

AavLEA1

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

T7

Availability

Academic Institutions and Nonprofits only

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PA-DAD-wt

Plasmid

#53095

Purpose

Expresses wild type PA-DAD in mammalian cells, an optogenetic tool to activate endogenous diaphanous related formins in live cells using light.

Depositor

Ronen Zaidel-Bar, Klaus Hahn

Article

Rao et al Cytoskeleton (Hoboken). 2013 Jul;70(7):3

Insert

LOV(wt)-6aa-DAD

Use

Tags

6x His and mVenus

Expression

Bacterial, Insect, and Mamm…

Mutation

L47F and C49S in Venus

Promoter

Availability

Academic Institutions and Nonprofits only

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JF001A

Plasmid

#53116

Purpose

expresses alpha hemolysin under the control of theophylline riboswitch

Depositor

Sheref Mansy

Article

Lentini et al Nat Commun. 2014 May 30;5:4012. doi: 10.

Insert

theophylline riboswitch and alpha hemolysin

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7 promoter

Availability

Academic Institutions and Nonprofits only

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pET28b-hUba2-His

Plasmid

#53117

Purpose

expression of full length human Uba2 with Cterminal His-tag in E.coli

Depositor

Frauke Melchior

Article

Werner et al Methods Mol Biol. 2009;497:187-99. doi:

Insert

UBA2 (UBA2 Human)

Use

Tags

His and T7 tag

Expression

Bacterial

Mutation

Promoter

T7

Availability

Academic Institutions and Nonprofits only

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pLenti-OC-IRES-BSD

Plasmid

#53118

Purpose

To create stable cell clones with high-level expression of Cas9 and OCT1

Depositor

Wensheng Wei

Article

Zhou et al Nature. 2014 Apr 9. doi: 10.1038/nature1

Inserts

OCT1 (POU2F1 Human)
Cas9

Use

Lentiviral

Tags

IRES-BSD, NLS, and P2A

Expression

Mammalian

Mutation

human codon-optimized

Promoter

CMV

Availability

Academic Institutions and Nonprofits only
pLenti-sgRNA-Lib

Plasmid

#53121

Purpose

sgRNA expression construction in a 3rd generation lentiviral backbone

Depositor

Wensheng Wei

Article

Zhou et al Nature. 2014 Apr 9. doi: 10.1038/nature1

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Availability

Academic Institutions and Nonprofits only

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pGfa2-EGFP

Plasmid

#53128

Purpose

Expression of EGFP in mouse astrocytes

Depositor

Michael Brenner

Insert

Gfa2 (GFAP Human)

Use

Mouse Targeting

Tags

EGFP and mP1

Expression

Mammalian

Mutation

contains -2163 to +47 (the human gfa2 segment), w…

Promoter

human Gfa2

Availability

Academic Institutions and Nonprofits only

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pGfaABC1D-nLac

Plasmid

#53131

Purpose

expression of lacZ driven by human GfaABC1D promoter

Depositor

Michael Brenner

Article

Lee et al Glia. 2008 Apr;56(5):481-93. doi: 10.100

Insert

Gfa2 (GFAP Human)

Use

Mouse Targeting

Tags

LacZ, NLS, and mP1

Expression

Mammalian

Mutation

contains ABC1D regions of Gfa2 promoter, -1757 to…

Promoter

human Gfa2

Availability

Academic Institutions and Nonprofits only

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pET28a-His-hAos1

Plasmid

#53135

Purpose

E.coli expression of full length human Aos1 (SAE1) with Nterminal His-tag

Depositor

Frauke Melchior

Article

Pichler et al Cell. 2002 Jan 11;108(1):109-20.

Insert

Aos1 (SAE1 Human)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

T7

Availability

Academic Institutions and Nonprofits only

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pET23a-mUbc9

Plasmid

#53137

Purpose

E.coli expression of full length untagged mouse Ubc9 (protein sequence identical to human)

Depositor

Frauke Melchior

Article

Pichler et al Cell. 2002 Jan 11;108(1):109-20.

Insert

Ubc9 (Ube2i Mouse)

Use

Tags

untagged

Expression

Bacterial

Mutation

protein sequence is identical to human

Promoter

T7

Availability

Academic Institutions and Nonprofits only

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pET11a-SUMO1

Plasmid

#53138

Purpose

E.coli expression of untagged mature human SUMO1 (amino acids 1-97)

Depositor

Frauke Melchior

Article

Pichler et al Cell. 2002 Jan 11;108(1):109-20.

Insert

SUMO1 (SUMO1 Human)

Use

Tags

Expression

Bacterial

Mutation

mature form (amino acids 1-97)

Promoter

T7

Availability

Academic Institutions and Nonprofits only

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pET11a-SUMO2

Plasmid

#53142

Purpose

E.coli expression of untagged mature human SUMO2 (amino acids 1-92)

Depositor

Frauke Melchior

Article

Flotho et al Methods Mol Biol. 2012;832:93-110. doi:

Insert

SUMO2 (SUMO3 Human)

Use

Tags

Expression

Bacterial

Mutation

mature form (amino acids 1-92)

Promoter

T7

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 SIRT6_H133Y

Plasmid

#53149

Purpose

expression vector for catalyitc inactive human Sirtuin6

Depositor

Katrin Chua

Article

Michishita et al Nature. 2008 Mar 27;452(7186):492-6. doi

Insert

Sirt6 (SIRT6 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

H133Y, catalytic inactive

Promoter

CMV

Availability

Academic Institutions and Nonprofits only
CD47-bio-His

Plasmid

#53150

Purpose

Expresses full-length CD47 ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

Depositor

Gavin Wright

Article

Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

Insert

CD47 (CD47 Human)

Use

Tags

His tag, enzymatic biotinylation sequence, and ra…

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 SIRT7_H187Y

Plasmid

#53151

Purpose

expression vector for catalytically inactive human Sirtuin7

Depositor

Katrin Chua

Article

Shin et al Cell Rep. 2013 Nov 14;5(3):654-65. doi:

Insert

Sirt7 (SIRT7 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

H187Y, catalytic inactive

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pBABEbleo-Flag-BRAFV600E

Plasmid

#53156

Purpose

Expresses Flag tagged human oncogenic BRAFV600E from bleomycin resistance retroviral vector

Depositor

Christopher Counter

Article

Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

Insert

BRAF (BRAF Human)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

V600E

Promoter

Availability

Academic Institutions and Nonprofits only

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pGEX6P1-HA-MEK1

Plasmid

#53159

Purpose

Expresses GST-HA tagged human MEK1 from bacterial expression vector

Depositor

Christopher Counter

Article

Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

Insert

MEK1 (MAP2K1 Human)

Use

Tags

GST and HA

Expression

Bacterial

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pWZLblasti-HA-ERK2 GOF

Plasmid

#53174

Purpose

Expresses HA tagged human ERK2 R67SD321N from blasticidin resistance retroviral vector

Depositor

Christopher Counter

Article

Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

Insert

ERK2 (MAPK1 Human, Mustard Weed)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

R67S/D321N

Promoter

Availability

Academic Institutions and Nonprofits only
pWZLblasti-HA-ERK5

Plasmid

#53175

Purpose

Expresses HA tagged human ERK5 from blasticidin resistance retroviral vector

Depositor

Christopher Counter

Article

Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

Insert

ERK5 (MAPK7 Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only