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Plasmid#40637DepositorInsertPOU5F1 (POU5F1 Human)
UseTagsGST, TEV cleavage, and Thrombin cleavageExpressionBacterialMutationT234APromotertacAvailable sinceJuly 26, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGEX4T-1_OCT4_S235E
Plasmid#40638DepositorInsertPOU5F1 (POU5F1 Human)
UseTagsGST, TEV Cleavage, and Thrombin CleavageExpressionBacterialMutationS235EPromotertacAvailable sinceJuly 26, 2013AvailabilityAcademic Institutions and Nonprofits only -
PcDNA-DEST53-CypB-Del4 N-term GFP
Plasmid#36137DepositorInsertcyclophilin B (PPIB Human)
UseTagsGFPExpressionMammalianMutationcontains amino acids 42-216 of cyclophilin BPromoterCMVAvailable sinceSept. 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
pHEE401_GV_MOD
Plasmid#194874PurposemCherry selection in Arabidopsis seeds in T1 and T2DepositorInsertCas9
UseCRISPRTagsExpressionMutationPromoteregg cell-specificAvailable sinceJan. 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-PHOSPHO2-TEV-Twin-Strep
Plasmid#222016PurposeExpress human PHOSPHO2 protein (C-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-fusion protein (ENLYFQGS-WSHPQFEK-(GGGS)2-GGSA-WSHPQFEK)) in mammalian cellDepositorInsertPHOSPHO2 (PHOSPHO2 Human)
UseTagsTEV cleavable site and Twin-Strep-tagExpressionMammalianMutationdeleted stop codon (TAA)Promoterchicken β-actin promoterAvailable sinceJan. 8, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGEX-PHOSPHO2-TEV-Twin-Strep
Plasmid#222017PurposeExpress human PHOSPHO2 protein (C-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-fusion protein (ENLYFQGS-WSHPQFEK-(GGGS)2-GGSA-WSHPQFEK)) in E. coliDepositorInsertPHOSPHO2 (PHOSPHO2 Human)
UseTagsTEV cleavable site and Twin-Strep-tagExpressionBacterialMutationdeleted stop codon (TAA)Promoterlac promoterAvailable sinceJan. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pFastBac SOX R139A
Plasmid#192058PurposeExpression vector for SOX (ORF37) R139ADepositorInsertSOX (ORF37)
UseTagsExpressionInsectMutationR139APromoterPolyhedrinAvailable sinceDec. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pACE WT SOX pACEmam1
Plasmid#192049PurposeExpresses WT SOX (pACEmam1)DepositorInsertWT SOX
UseUnspecifiedTagsExpressionMutationPromoterCMVAvailable sinceDec. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFastBac SOX Q129H
Plasmid#192057PurposeExpression vector for SOX (ORF37) Q129HDepositorInsertSOX (ORF37) Q129H
UseTagsExpressionInsectMutationQ129HPromoterPolyhedrinAvailable sinceDec. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEJS2701-CMV-Nme2Smu-ABE8e-nt-E932R
Plasmid#228722PurposeExpresses N-terminal ABE8e-Nme2Smu nCas9 (D16A) base editor with E932R mutation in mammalian cellsDepositorInsertN-terminal ABE8e with Nme2Smu nCas9 (D16A) E932R
UseCRISPRTagsExpressionMammalianMutationNme2-Cas9, Smu PID, D16A E932RPromoterCMVAvailable sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEJS2702-CMV-iNme2-ABE8e-nt
Plasmid#228723PurposeExpresses N-terminal ABE8e-iNme2 nCas9 (D16A) base editor in mammalian cellsDepositorInsertN-terminal ABE8e with iNme2 nCas9 (D16A)
UseCRISPRTagsExpressionMammalianMutationNme2-Cas9 D16A D844G E868K K870R D873A D911G K929…PromoterCMVAvailable sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEJS2700-CMV-Nme2-ABE8e-nt-E932R
Plasmid#228721PurposeExpresses N-terminal ABE8e-Nme2 nCas9 (D16A) base editor with E932R mutation in mammalian cellsDepositorInsertN-terminal ABE8e with Nme2 nCas9 (D16A) E932R
UseCRISPRTagsExpressionMammalianMutationNme2-Cas9 D16A E932RPromoterCMVAvailable sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEJS2712-CMV-eNme2-C-ABE8e-i1-L10
Plasmid#228733PurposeExpresses domain inlaid eNme2-C nCas9 (D16A)-i1-ABE8e base editor with linker-10 in mammalian cellsDepositorInsertDomain inlaid eNme-C nCas9 (D16A)-i1-L10
UseCRISPRTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Nuc-deltaCKAR
Plasmid#222430PurposeFRET-based reporter for monitoring deltaPKC activity at the nucleus in cells.DepositorInsertNucleus delta-selective C kinase activity reporter
UseTagsCFP and YFPExpressionMammalianMutationPromoterAvailable sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
T2A GFP H2B
Plasmid#227722PurposePlasmid contains T2A GFP H2B, with some nlp-51 homology sequence before, Amp ResistantDepositorInsert(nlp-51 homology) T2A 3xNLS GFP (nlp-51 Nematode)
UseCloning vectorTagsT2A 3xNLS GFPExpressionMutationPromoterAvailable sinceNov. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
T2A 3xNLS GFP
Plasmid#227721PurposePlasmid contains T2A 3xNLS GFP, with some nlp-51 homology sequence before, Amp ResistantDepositorInsertT2A 3xNLS GFP with some nlp-51 homology sequence (nlp-51 Nematode)
UseCloning vectorTagsExpressionMutationPromoterAvailable sinceNov. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
Sc++
Plasmid#198545PurposeExpresses human codon-optimized Sc++ and blasticidin resistance: EFS promoter-Sc++-NLS-FLAG-P2A-BSDDepositorInsertSc++
UseCRISPR and LentiviralTagsBSD, FLAG, and NLSExpressionMammalianMutationPromoterEFSAvailable sinceNov. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
SpG
Plasmid#198543PurposeExpresses human codon-optimized SpG and blasticidin resistance: EFS promoter-SpG-NLS-FLAG-P2A-BSDDepositorInsertSpG
UseCRISPR and LentiviralTagsBSD, FLAG, and NLSExpressionMammalianMutationPromoterEFSAvailable sinceNov. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Golgi-deltaCKAR
Plasmid#222428PurposeFRET-based reporter for monitoring deltaPKC activity at the Golgi in cells.DepositorInsertGolgi delta-selective C kinase activity reporter
UseTagsCFP and YFPExpressionMammalianMutationPromoterAvailable sinceNov. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBbA5c-cdl
Plasmid#207649PurposeOverexpression of heterologous L-cysteine desulfidase in E. coli MG1655DepositorInsertcdl
UseTags6xHisExpressionBacterialMutationPromoterlacUV5Available sinceOct. 2, 2024AvailabilityAcademic Institutions and Nonprofits only