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Plasmid#132298PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectorsDepositorInsertUNC93B1 (UNC93B1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 12, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLeAPS-GFP-blast
Plasmid#182230PurposeLentiviral transfer plasmid for the LeAPS packaging system; encodes GFP-P2A-blasticidin transgeneDepositorInsertGFP-P2A-Blasticidin
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLPCX mito Grx1-roGFP2
Plasmid#64977PurposeMammalian expression of mitochondrial Grx1-roGFP2 (retroviral vector)DepositorInsertGlutaredoxin-1 (GLRX Human)
UseRetroviralTagsroGFP2ExpressionMammalianMutationmitochondrial targeting sequencePromoterAvailable sinceJune 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
N174-MCS (Puro)
Plasmid#81068PurposeLentiviral vector for constitutive gene expression.DepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterEF-1αAvailable sinceAug. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
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pcDNA-puro-TagBFP-β-ACTIN-3′UTR-8xMS2
Plasmid#205160Purposeexpress β-ACTIN-3′UTR-8xMS2DepositorInsertβ-ACTIN-3′UTR-24xMS2
UseTagstagBFPExpressionBacterial and MammalianMutationPromoterCMVAvailable sinceOct. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLVX-EGFP-C1-centrin-1
Plasmid#73331PurposeLentiviral vector encoding centrin-1 with an N-terminal EGFP tagDepositorInsertcentrin-1 (CETN1 Human)
UseLentiviralTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-ALK7 T194D
Plasmid#80886Purposemammalian expression of ALK7 T194DDepositorInsertALK7 (Acvr1c Rat)
UseTagsHAExpressionMammalianMutationT194D (constitutively active)PromoterCMVAvailable sinceAug. 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-2xFLAG-SREBP-1a
Plasmid#26801DepositorInsertSREBP-1a (SREBF1 Human)
UseTags2xFLAGExpressionMammalianMutationPromoterAvailable sinceMarch 18, 2011AvailabilityAcademic Institutions and Nonprofits only -
pSLIK-Venus
Plasmid#25734PurposeLentiviral vector for Tet-based inducible shRNA or cDNA expression, constitutive Venus fluorescent protein coexpression.DepositorTypeEmpty backboneUseLentiviral and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceJuly 1, 2010AvailabilityAcademic Institutions and Nonprofits only -
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pSFFV_mNG3A(1-10)
Plasmid#157992PurposeLentiviral vector of constitutive expression of mNeonGreen3A(1-10)DepositorInsertpSFFV_mNG3K(1-10)
UseLentiviralTagsExpressionMammalianMutationW148L, M150V, K153M, N194K, F204LPromoterSFFVAvailable sinceOct. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-PVR-Fc(DAPA)-AviTag-6xHis
Plasmid#156765PurposeMammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.DepositorInsertPVR (PVR Human)
UseTagsFc(DAPA)-AviTag-6xHisExpressionMammalianMutationFc region of human IgG contains D265A and P329A m…PromoterCMV/SP6Available sinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBPhsFlp2::PEST
Plasmid#63171PurposeDrosophila codon-optimized Flp2::mODC-PEST fusion gene driven from Drosophila Hsp70Bb Heat Shock PromoterDepositorInserthsFlp2::PEST
UseTagsExpressionInsectMutationPromoterAvailable sinceMay 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDest eGFP MBNL1 42 kDa
Plasmid#61277PurposeMammalian expression of EGFP-tagged human MBNL1DepositorInsertMBNL1 (MBNL1 Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceDec. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
myc-mTOR (1750-2549 aa)
Plasmid#21747DepositorInsertmTOR (1750-2549) (Mtor Rat)
UseTagsmycExpressionMammalianMutationamino acids 1750-2549PromoterAvailable sinceAug. 12, 2009AvailabilityAcademic Institutions and Nonprofits only -
pAM-AAV-mSyn1-EGFP
Plasmid#153187PurposeMouse synapsin-1 (mSyn1) promoter-mediates gene expression in retinal neurons with fluorescent reporterDepositorTypeEmpty backboneUseAAVTagsExpressionMammalianMutationPromoterAvailable sinceJune 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
DBDmut-CA-RIT-NFAT1
Plasmid#63669PurposeConstitutively active NFAT1 and also unable to interact with AP-1 with four point mutations in the DNA binding loop that abolish DNA bindingDepositorInsertNFAT1 (Nfatc2 Mouse)
UseRetroviralTags3x HAExpressionMammalianMutationCA: PASSGSSASF mutated to PAAAGAAAAF, SPRTSPIMSP…PromoterAvailable sinceJuly 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCAG-Brainbow3.0
Plasmid#45176DepositorInsertpCAG-Brainbow3.0
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 27, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-Wnt1-V5
Plasmid#35924DepositorInsertWnt1 (WNT1 Human)
UseTagsV5ExpressionMammalianMutationNonePromoterCMVAvailable sinceMay 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
pOpen-ETSSB
Plasmid#165549PurposessDNA binding protein, 9kDa. Features: Improves the processivity of DNA polymerase; involved in stabilization and marking of ssDNA structure; increases the yield and specificitiy of PCR; increases the yield and processivity of RT during RT-PCR; improves DNA sequencing through regions with strong secondary structureDepositorInsertExtreme Thermostable Single-Stranded DNA Binding Protein
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 10, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCAG-G-Flamp1-mut
Plasmid#188568Purposecontrol probe of G-Flamp1, insensitive to cAMPDepositorInsertG-Flamp1-mut
UseTagsHis tagExpressionMammalianMutationPromoterCAGAvailable sinceNov. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLL1 sgRNA(MS2)-MS2-AIDmono
Plasmid#112127Purposeempty sgRNA cloning vector with MS2-AIDmonoDepositorInsertAID mono mutant (AICDA Human)
UseCRISPRTagsExpressionMammalianMutationN- mutated and C- terminal truncated, H130A/R131E…PromoterhU6,CBhAvailable sinceSept. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pgRNA-modified
Plasmid#84477Purposeguide RNA expression lentiviral constructDepositorInsertguide RNA
UseLentiviralTagsExpressionMammalianMutationPromotermU6Available sinceNov. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBABE-Puro-KRas*
Plasmid#46745PurposeExpresses human KRas protein from chimeric cDNA sequence derived primarily from HRAS codons with a KRAS 3' tail. Results in higher than normal exogenous expression of KRas protein.DepositorInsertKRAS* (KRAS Synthetic, Human)
UseRetroviralTagsFLAGExpressionMammalianMutationcDNA is encoded by a chimeric mixture of HRAS and…PromoterAvailable sinceAug. 20, 2013AvailabilityAcademic Institutions and Nonprofits only -
CMV500 empty vector
Plasmid#33348DepositorTypeEmpty backboneUseTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceDec. 9, 2011AvailabilityAcademic Institutions and Nonprofits only -
Gal:mCherry-uTEV3
Plasmid#135453PurposeYeast expression of uTEV3 fused to mCherryDepositorInsertmCherry-uTEv3
UseTagsmCherryExpressionYeastMutationS219V mutation improves stability , I138T/S153N/T…PromoterGalAvailable sinceJan. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-miRFP680-P2A-GFP
Plasmid#197164PurposeExpresses the protein of miRFP680-P2A-GFP in mammalian cellsDepositorInsertmiRFP680-P2A-GFP
UseAAVTagsExpressionMutationPromoterAvailable sinceMarch 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
YIplac211-Vph1-HaloTag
Plasmid#140149PurposePlasmid for pop-in/pop-out replacement of Vph1 with Vph1-HaloTagDepositorInsertVPH1
UseTagsExpressionYeastMutationPromoterAvailable sinceSept. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CMV-Pink Flamindo-p2a-bPAC
Plasmid#202716PurposeMammalian co-expression of soluble Pink Flamindo, a fluorescent cAMP indicator and soluble bPAC, a photoactivatable adenylyl cyclaseDepositorArticleInsertsPink Flamindo
bPAC
UseLentiviralTags10xHis and mycExpressionMammalianMutationPromoterCMVAvailable sinceAug. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
p-dCas9-JMJD2A-Hygro
Plasmid#104410Purposetransient expression of dCas9-JMJD2A fusion proteinDepositorInsertJMJD2A (KDM4A Human)
UseCRISPRTagsExpressionMammalianMutationPromoterCMV promoterAvailable sinceApril 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-ERK-SPARK
Plasmid#106921PurposeExpress ERK-SPARK, an activity reporter of ERK in mammalian cells.DepositorInsertERK-SPARK
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
KBB364
Plasmid#185083PurposeRFA1 fused to GFP under control of GAL1 promoterDepositorInsertRFA1
UseTagsGFPExpressionYeastMutationPromoterGAL1Available sinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUltra-Plus-H2B-mTagBFP2
Plasmid#161790Purpose3rd generation lentiviral bicistronic vector for mammalian expression of H2B-mTagBFP2 and one gene of interestDepositorInsertH2B-mTagBFP2
UseLentiviral; BicistronicTagsmTagBFP2ExpressionMammalianMutationI174A in the mTagBFP2 protein according to PLoS O…PromoterUbC and UbC promoterAvailable sinceNov. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMXsIP GFP-VMP1
Plasmid#117417PurposeExpresses VMP1 tagged with GFP in mammalian cellsDepositorInsertVMP1 (VMP1 Human)
UseRetroviralTagsGFPExpressionMammalianMutationPromoterAvailable sinceOct. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLenti-mCherry-CDK4KTR
Plasmid#126680PurposeFluorescent reporter for CDK4/6 activityDepositorInsertRB cterm
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceApril 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMK286 (mAID-NeoR)
Plasmid#72824PurposeC-terminal tagging with mAID using CRISPR/Cas9DepositorInsertmAID-Neo
UseCRISPRTagsmAIDExpressionMammalianMutationPromoterAvailable sinceMarch 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDEST_FLAG-HUWE1_UBA/UIM-mut
Plasmid#187152Purposemammalian cell expression of FLAG-HUWE1 M1328A/F1330A/A1376G/S1382ADepositorInsertHUWE1 (HUWE1 Human)
UseTags1xFLAGExpressionMammalianMutationM1328A/F1330A/A1376G/S1382APromoterAvailable sinceAug. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-ccdB-3xFLAG
Plasmid#214984PurposeGateway compatible empty vector (transient mammalian expression) with a C-terminal 3xFLAG tagDepositorTypeEmpty backboneUseTags3xFLAGExpressionMammalianMutationPromoterAvailable sinceMarch 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
CMV10-3xFlag Skp2 WT
Plasmid#81115Purposemammalian expression of Skp2 WTDepositorInsertSKP2 (SKP2 Human)
UseTags3xFlagExpressionMammalianMutationPromoterCMVAvailable sinceSept. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCW57.1-Neurod1-3xT7
Plasmid#200890PurposeInducible lentiviral expression of Neurod1DepositorInsertNeurod1 (Neurod1 Mouse)
UseLentiviral; Doxycycline inducibleTags3xT7ExpressionMammalianMutationPromoterTRE promoter, Tet ONAvailable sinceJune 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLentiCRISPRv2-TRAF6-targeting sgRNA 1
Plasmid#131345PurposegRNA targeting mouse TRAF6DepositorInsertgRNA targeting mouse TRAF6 (Traf6 Mouse)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceSept. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLentiCRISPRv2-TRAF6-targeting sgRNA 2
Plasmid#131346PurposegRNA targeting mouse TRAF6DepositorInsertgRNA targeting mouse TRAF6 (Traf6 Mouse)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceSept. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
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TFORF2408
Plasmid#143370PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertFOXF1 (FOXF1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJuly 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
plenti-UBC-Myc-gate-pGK-HYG
Plasmid#107395PurposeLentiviral gateway destination vectorDepositorTypeEmpty backboneUseLentiviral; Gateway destinationTagsMycExpressionMammalianMutationPromoterAvailable sinceMay 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
TALED_ Left-mouse-mtRnr1_A1555G-8e-E1347A
Plasmid#212949PurposeExpresses TALED_ Left-mouse-mtRnr1_A1555G-8e-E1347A in mammalian cellsDepositorInsertTALED_ Left-mouse-mtRnr1_A1555G-8e-E1347A (mt-Rnr1 Synthetic)
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMx-puro-MGT
Plasmid#111809PurposeRetroviral expression vector for direct cardiac reprogrammingDepositorUseRetroviralTagsExpressionMammalianMutationPromoterviral LTRAvailable sinceAug. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
Piggybac-TetO-hNurr1-blast
Plasmid#176485PurposeTetO-hNurr1 in a PiggyBac vector with Blasticidin resistanceDepositorInsertNurr1 (NR4A2 Human)
UseTagsExpressionMammalianMutationPromoterTetOAvailable sinceMarch 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
GFP-PIK3C2B
Plasmid#161989PurposeMammalian expression vector containing PIK3C2B tagged with GFPDepositorInsertPIK3C2B (PIK3C2B Human)
UseTagsGFPExpressionMammalianMutationsiRNA resistance to siRNA#2 with 4 silent mutatio…PromoterAvailable sinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only