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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 26720 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepDsRed-N1
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Backbone manufacturerClontech
- Backbone size w/o insert (bp) 4700
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Vector typeMammalian Expression, Bacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameZyxin
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SpeciesH. sapiens (human); unknown
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Entrez GeneZYX (a.k.a. ESP-2, HED-2)
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Tag
/ Fusion Protein
- RFP (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site HindIII (not destroyed)
- 5′ sequencing primer n/a (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
RFP-zyxin was a gift from Anna Huttenlocher (Addgene plasmid # 26720 ; http://n2t.net/addgene:26720 ; RRID:Addgene_26720) -
For your References section:
Regulation of focal complex composition and disassembly by the calcium-dependent protease calpain. Bhatt A, Kaverina I, Otey C, Huttenlocher A. J Cell Sci. 2002 Sep 1. 115(Pt 17):3415-25. PubMed 12154072