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Addgene

pEGFP-N1 alpha-actinin 1
(Plasmid #11908)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 11908 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pEGFP-N1
  • Backbone manufacturer
    Clontech Laboratories
  • Backbone size w/o insert (bp) 4700
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    alpha-actinin 1
  • Alt name
    ACTN1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2685
  • GenBank ID
    NM_001102
  • Entrez Gene
    ACTN1 (a.k.a. BDPLT15)
  • Tag / Fusion Protein
    • eGFP (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer EGFP-N Sequencing Primer
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Dr. D.J. Kwiatkowski (Harvard Medical School) provided non-muscle alpha-actinin cDNA.
  • Articles Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEGFP-N1 alpha-actinin 1 was a gift from Carol Otey (Addgene plasmid # 11908 ; http://n2t.net/addgene:11908 ; RRID:Addgene_11908)
  • For your References section:

    Dynamics of alpha-actinin in focal adhesions and stress fibers visualized with alpha-actinin-green fluorescent protein. Edlund M, Lotano MA, Otey CA. Cell Motil Cytoskeleton. 2001 Mar . 48(3):190-200. 10.1002/1097-0169(200103)48:3<190::AID-CM1008>3.0.CO;2-C PubMed 11223950