-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 11908 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepEGFP-N1
-
Backbone manufacturerClontech Laboratories
- Backbone size w/o insert (bp) 4700
-
Vector typeMammalian Expression
-
Selectable markersNeomycin (select with G418)
Growth in Bacteria
-
Bacterial Resistance(s)Kanamycin, 50 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert namealpha-actinin 1
-
Alt nameACTN1
-
SpeciesH. sapiens (human)
-
Insert Size (bp)2685
-
GenBank IDNM_001102
-
Entrez GeneACTN1 (a.k.a. BDPLT15)
-
Tag
/ Fusion Protein
- eGFP (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII (not destroyed)
- 3′ cloning site HindIII (not destroyed)
- 5′ sequencing primer CMV-F
- 3′ sequencing primer EGFP-N Sequencing Primer (Common Sequencing Primers)
Resource Information
-
A portion of this plasmid was derived from a plasmid made byDr. D.J. Kwiatkowski (Harvard Medical School) provided non-muscle alpha-actinin cDNA.
-
Articles Citing this Plasmid
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pEGFP-N1 alpha-actinin 1 was a gift from Carol Otey (Addgene plasmid # 11908 ; http://n2t.net/addgene:11908 ; RRID:Addgene_11908) -
For your References section:
Dynamics of alpha-actinin in focal adhesions and stress fibers visualized with alpha-actinin-green fluorescent protein. Edlund M, Lotano MA, Otey CA. Cell Motil Cytoskeleton. 2001 Mar . 48(3):190-200. 10.1002/1097-0169(200103)48:3<190::AID-CM1008>3.0.CO;2-C PubMed 11223950