pLX304-KDM6A
(Plasmid #203580)

• Purpose: Expresses wild-type KDM6A in mammalian cells.
• Depositing Labs: Abhishek Chakraborty, William Kaelin
• Publication: Chakraborty et al Science. 2019 Mar 15;363(6432):1217-1222. doi: 10.1126/science.aaw1026. Epub 2019 Mar 14.

Backbone

• Vector backbone: pLX304
• Backbone size w/o insert (bp): 4452
• Total vector size (bp): 12012
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Blasticidin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: KDM6A
• Alt name: UTX
• Alt name: KABUK2
• Alt name: Lysine demethylase 6A
• Species: H. sapiens (human)
• Insert Size (bp): 4452
• GenBank ID: 7403
• Entrez Gene: KDM6A (a.k.a. KABUK2, UTX, bA386N14.2)
• Promoter: CMV

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: WPRE-R

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

KDM6A was sub-cloned from pCMV-HA-UTX construct (addgene #24168) using gateway cloning.