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Plasmid#73081PurposeLentiviral transfer vector for tet-inducible expression of EGFP tagged human RHOA-Q63LDepositorInsertRHOA-Q63L (RHOA Human)
UseLentiviralTagseGFPExpressionMammalianMutationQ63L Constitutively activePromoterAvailable sinceFeb. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
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pAAV-Puro_siKO
Plasmid#86696PurposeTetracycline inducible expression of guide RNA targeted to AAVS1 locusDepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterH1 TOAvailable sinceJan. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCAG-dGBP1-TagBFP
Plasmid#80086PurposeGFP-dependent dGBP1-TagBFP, for expression in mammalian cellsDepositorInsertdGBP1-TagBFP
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceDec. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
GPI-mCherry2
Plasmid#182869PurposeMonomer expression vector with plasma membrane localization signalDepositorInsertGPI-mCherry2
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAMS823 His6-MBP-3C-ORF2p (238-1061, ORFeus-Hs) in pET41
Plasmid#213024PurposeExpresses human LINE-1 ORF2p Core (238-1061) in E. coli as an N-MBP fusionDepositorInsertLINE-1 ORF2p (238-1061)
UseTagsHis6-MBP-3CExpressionBacterialMutationPromoterT7 / LacAvailable sinceMay 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBE14
Plasmid#174653PurposeIn vivo Cre-lox circularization in E. coliDepositorInsertCre
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSSRB274_pAC8-eGFP-hsCRBN
Plasmid#218791PurposeInsect cell expression vector for FLAG-TEV-eGFP-Prescission-hsCRBNDepositorInsertProtein cereblon (CRBN Human)
UseTagsFLAG-TEV-eGFP-PrescissionExpressionInsectMutationeGFP fused to N-terminusPromoterpolyhedrinAvailable sinceMay 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
TFORF2214
Plasmid#142176PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertCREB3L4 (CREB3L4 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJL1-eBFP2
Plasmid#102640PurposeIn vitro expression of eBFP2 from the T7 promoterDepositorInserteBFP2
UseTagsExpressionBacterialMutationPromoterT7Available sinceAug. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-ApoE3
Plasmid#197147PurposeExpresses ApoE3 in Mammalian cellDepositorInsertApoE3 (APOE Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHAGE2 Lnp-mCherry
Plasmid#86687PurposeLentivirus to stably express fluorescent human protein in mammalian cellsDepositorInsertLNPK (LNPK Human)
UseLentiviralTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET28a-SpyTag002-MBP
Plasmid#102831PurposeBacterial expression of SpyTag002 fused to maltose binding proteinDepositorInsertSpyTag002-MBP
UseTagsHis6 and Thrombin cleavage siteExpressionBacterialMutationPeptide tag added to the N-terminus of MBP via a …PromoterT7Available sinceDec. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEP4 E02S EN2K
Plasmid#20925Purposeused in the derivation of human iPS cells using non-integrating episomal vectors; expresses Oct4 and Sox2; Nanog and Klf4DepositorArticleUseEpisomal/ebnaTagsExpressionMutationPromoterAvailable sinceMay 20, 2009AvailabilityAcademic Institutions and Nonprofits only -
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pcDNA3 mouse IL-6 (1240)
Plasmid#66908PurposeMammalian expression of IL-6DepositorInsertIL-6
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
AAV-CAGGS-EGFP
Plasmid#22212PurposeAAV zinc finger donor vector for genomic targetingDepositorInsertCAGGS-eGFP
UseZfn donorTagsExpressionMutationPromoterAvailable sinceOct. 14, 2009AvailabilityAcademic Institutions and Nonprofits only -
PCBP1_Full-length_pGEX
Plasmid#135108PurposeRecombinant expression of PCBP1 with dual-affinity tagDepositorInsertPCBP1 (PCBP1 Human)
UseTagsGST-SBPExpressionBacterialMutationPromotertacAvailable sinceNov. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDONR221_SLC3A2
Plasmid#132213PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectorsDepositorInsertSLC3A2 (SLC3A2 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 4, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pMH0001
Plasmid#85969PurposeUCOE-SFFV-dCas9-BFP-KRABDepositorInsertdCas9
UseLentiviralTagsBFP-KRABExpressionMammalianMutationD10A, H840APromoterSFFVAvailable sinceFeb. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHLmMBP-10
Plasmid#72348PurposeMammalian expression of secreted N-terminally 8His-tagged mMBP TEV cleavage site-linked fusion proteins with C-terminal 6His-tag/Strep-tag II/HA-tagDepositorTypeEmpty backboneUseTags6His-tag, 8His-tag, HA-tag, Strep-tag II, and mMB…ExpressionMammalianMutationPromoterCMV enhancer + chicken beta-actin promoterAvailable sinceFeb. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
AbVec2.0-mIgkc
Plasmid#127157PurposeExpression of immunoglobulin light chains in mammalian cells, mouse (C57BL/6), kappa constant regionDepositorInsertIgkc (Igkc Mouse)
UseTagsExpressionMammalianMutationPromoterhCMV IE1 gene promoterAvailable sinceNov. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
Flag-FOXP3
Plasmid#153147PurposeExpression of Flag tagged human FOXP3 in mammalian cellsDepositorInserthuman FOXP3 (FOXP3 Human)
UseTagsFlagExpressionMammalianMutationPromoterCMV IE94Available sinceAug. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-iFlpV
Plasmid#140137Purposecan be used to generate AAV virus that will express light-inducible site-specific iFlpV recombinaseDepositorHas ServiceAAV PHP.eB and AAV1InsertiFlpV
UseAAVTagsExpressionMutationPromoterEF1aAvailable sinceApril 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLenti PGK Hygro DEST (w530-1)
Plasmid#19066Purpose3rd gen lentiviral Gateway destination vector, expression, PGK promoter, HygroDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 12, 2009AvailabilityAcademic Institutions and Nonprofits only -
pREDawn-AmpR-MCS
Plasmid#188978PurposeFor red light-induced bacterial expression of gene of interest (AmpR)DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAVS1-P-CAG-GFP
Plasmid#80491Purposedonor vector for AAVS1 targeting (puromycin selection) and constitutive GFP expressionDepositorInsertCAG-GFP
UseDonor vector (human)TagsExpressionMammalianMutationPromoterCAGAvailable sinceNov. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-TetON-Puro-HIF1a 0819
Plasmid#118704PurposeLentivirus for expression of shRNA1 against human HIF1a (Dox-inducible)DepositorInsertshRNA against human HIF1a
UseLentiviralTagsExpressionMutationPromoterAvailable sinceNov. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBAD-LSSmOrange
Plasmid#37129DepositorInsertLSSmOrange
UseTagsHisExpressionBacterialMutationA44V/F84L/W145M/I165D/M167L/G202D compared to mOr…PromoteraraBAvailable sinceJune 5, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1- dCas9-2xNLS-EGFP
Plasmid#74710PurposeExpresses dCas9-2xNLS-EGFP in mammalian cellsDepositorInsertdCas9-2xNLS-EGFP
UseCRISPRTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceApril 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCMV-RdLight1
Plasmid#125706PurposeExpresses the red genetically encoded dopamine sensor RdLight1 in mammalian cells.DepositorInsertRdLight1
UseTagsFlag tagExpressionMammalianMutationPromoterCMVAvailable sinceJuly 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV-GR11
Plasmid#89105Purposemammalian expression of full-length human glucocorticoid receptor in pCMV5DepositorInserthuman glucocorticoid receptor (NR3C1 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLV-tetO-mCherry
Plasmid#70273PurposeLentiviral plasmid for tetracycline/doxycycline dependent expression of mCherryDepositorInsertmCherry
UseLentiviralTagsExpressionMammalianMutationPromotertetOAvailable sinceNov. 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
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pBR_attB(bxb)_lox
Plasmid#183762PurposeBxb1-specific donor plasmid for the cloning of payloads <20 kbDepositorTypeEmpty backboneUseCre/Lox and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceSept. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pNTI825
Plasmid#216633PurposeCal-ID (V5) for AAV productionDepositorArticleInsertCal-ID
UseAAVTagsV5ExpressionMammalianMutationPromoterAvailable sinceApril 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCS6-YFP-SMASh
Plasmid#68853PurposeExpresses SMASh tag fused to YFP at C terminus in mammalian cellsDepositorInsertYFP-SMASh
UseTagsSMASh tagExpressionMammalianMutationPromoterCMVAvailable sinceSept. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
TRPC1-FLAG
Plasmid#24408DepositorInsertTRPC1 (TRPC1 Human)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceAug. 20, 2010AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.3-NL63-D14
Plasmid#172666Purposeencodes NL63 spike protein, for pseudovirus productionDepositorInsertHCNV63gp2 (HCNV63gp2 human coronavirus NL63)
UseLentiviral and RetroviralTagsNAExpressionMammalianMutationc-terminal deletion of 14aaPromoterCMVAvailable sinceJan. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCRISPRyl
Plasmid#70007PurposeCRISPR/Cas9 vector for Yarrowia lipolytica, with AvrII site for sgRNA insertionDepositorInsertsCodon optimized Cas9 from S. pyogenes
sgRNA expression cassette
UseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterSCR1'-tRNA and UAS1B8-TEF(136)Available sinceFeb. 16, 2016AvailabilityAcademic Institutions and Nonprofits only