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PurposeBinary vector for expressing nuclear miniTurbo-YFP under the UBQ10 promoter in plants
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 127370 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneR4pGWB601
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Backbone manufacturerhttp://shimane-u.org/nakagawa/gbv.htm
- Total vector size (bp) 12680
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Vector typePlant Expression
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Selectable markersBasta
Growth in Bacteria
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Bacterial Resistance(s)Spectinomycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameminiTurbo (BirA mutant)
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SpeciesBirA from E. coli, codon optimized for human and mutated
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Insert Size (bp)771
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Mutationaa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V160A, T192A, K194I, M209V, I305V
- Promoter Arabidopsis UBQ10 promoter
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Tags
/ Fusion Proteins
- V5 (N terminal on insert)
- GS linker (C terminal on insert)
- YFP (C terminal on insert)
- NLS (C terminal on insert)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer M13 fw
- 3′ sequencing primer M13 rv (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made byminiTurbo was obtained from Alice Ting's lab at Stanford University (cloned from V5-miniTurbo-NES_pCDNA3, Addgene catalog #107170).
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
R4pGWB601_UBQ10p-miniTurbo-YFP-NLS was a gift from Dominique Bergmann (Addgene plasmid # 127370 ; http://n2t.net/addgene:127370 ; RRID:Addgene_127370) -
For your References section:
Proximity labeling of protein complexes and cell type-specific organellar proteomes in Arabidopsis enabled by TurboID. Mair A, Xu SL, Branon TC, Ting AY, Bergmann DC. Elife. 2019 Sep 19;8. pii: 47864. doi: 10.7554/eLife.47864. 10.7554/eLife.47864 PubMed 31535972