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Plasmid#123025PurposePheromone responsive promoterDepositorInsertpFIG1 [2]
UseTagsExpressionYeastMutationPromoterAvailable sinceApril 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pWS060
Plasmid#123024PurposePheromone responsive promoterDepositorInsertpFUS1 [2]
UseTagsExpressionYeastMutationPromoterAvailable sinceApril 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSTARR-seq_fly-pnr
Plasmid#71503PurposeflySTARR-seq screening vector with pnr core promoterDepositorTypeEmpty backboneUseFlystarr-seq screening vectorTagssgGFP (SuperGlo, Qbiogene, Inc)ExpressionInsectMutationPromoterAvailable sinceDec. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pASKt15C+SrtA2
Plasmid#64984PurposeExpresses the E105K/E108A mutant of Staphylococcus aureus sortase A in E. coliDepositorInsertsortase A
UseTagsHis6 tag and S tagExpressionBacterialMutationdeleted amino acids 1-59, E105K, E108APromotertetracycline promoter/operatorAvailable sinceJune 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSTAP-seq_fly-ctrl
Plasmid#86380PurposeVector to measure the basal activity of CP candidates from a genomic library (cloned instead of the core promoter) by determining the abundance of transcripts originating from each candidate.DepositorTypeEmpty backboneUseStap-seq screening vectorTagsExpressionMutationPromoterAvailable sinceMarch 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSTARR-seq_fly-NipB
Plasmid#71502PurposeflySTARR-seq screening vector with NipB core promoterDepositorTypeEmpty backboneUseFlystarr-seq screening vectorTagssgGFP (SuperGlo, Qbiogene, Inc)ExpressionInsectMutationPromoterAvailable sinceDec. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDGE65
Plasmid#79447Purposerecipient plasmid [single nuclease]; pPcUbi:Cas9, BASTADepositorInsertccdB (ccdB(letD) )
UseCRISPRTagsExpressionPlantMutationBsaI site eliminatedPromoterAvailable sinceSept. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDGE146
Plasmid#79449Purposerecipient plasmid [multiplexing]; pPcUbi:Cas9, FAST, BASTADepositorInsertccdB (ccdB(letD) )
UseCRISPRTagsExpressionPlantMutationBsaI site eliminatedPromoterAvailable sinceSept. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDGE167
Plasmid#79457Purposerecipient plasmid [multiplexing]; pPcUbi:Cas9, FAST, HygroDepositorInsertccdB (ccdB(letD) )
UseCRISPRTagsExpressionPlantMutationBsaI site eliminatedPromoterAvailable sinceSept. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDGE168
Plasmid#79458Purposerecipient plasmid [multiplexing]; pDD45:Cas9, FAST, HygroDepositorInsertccdB (ccdB(letD) )
UseCRISPRTagsExpressionPlantMutationBsaI site eliminatedPromoterAvailable sinceSept. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAC-EsaR-I70V/D91G
Plasmid#47644PurposepAC-EsaR with a208g and a272g mutations in esaRDepositorInsertesaR-I70V/D91G
UseSynthetic BiologyTagsExpressionBacterialMutationChanged Isoleucine 70 to Valine and Aspartic acid…PromoterPlacAvailable sinceSept. 6, 2013AvailabilityAcademic Institutions and Nonprofits only -
pTBL3481 MTK3-mCherry
Plasmid#226672PurposeMTK3 part containing the coding sequence of mCherry.DepositorInsertmCherry
UseSynthetic BiologyTagsExpressionMammalianMutationRemoved BbsI sitesPromoternoneAvailable sinceNov. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTBL2670 MTK3-PEmax
Plasmid#226663PurposeMTK3 part containing the coding sequence of PEmaxDepositorInsertPEmax
UseCRISPR and Synthetic BiologyTagsExpressionMutationPromoternoneAvailable sinceNov. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pACYCDuet-Myc-MBP-HA
Plasmid#222711PurposeExpression Myc-tagged F-box protein and MBP-HA-tagged substrate in bacterial cellsDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
dCas9 fused to nanobody (anti-beta catenin)
Plasmid#186420PurposeExpression of dCas9 with C-terminal nanobody fusion recognizing beta catenin tagDepositorInsertdCas9-nanobody fusion (anti-beta catenin)
UseCRISPRTags6HisExpressionMutationPromoterAvailable sinceOct. 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBbA5c-cdl
Plasmid#207649PurposeOverexpression of heterologous L-cysteine desulfidase in E. coli MG1655DepositorInsertcdl
UseTags6xHisExpressionBacterialMutationPromoterlacUV5Available sinceOct. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pACYC-cdl
Plasmid#207652PurposeOverexpression of heterologous L-cysteine desulfidase in E. coli MG1655DepositorInsertcdl
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceOct. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLPE3
Plasmid#209985PurposeContains Level 0* Part: E. coli shuttle part (pMB1_cat) for the construction of Level 2 plasmidsDepositorInsertE. coli shuttle part (pMB1_cat)
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHBJT006
Plasmid#225157PurposeLow copy cloning vector for integration of C-terminal FLAG epitope tag (ChlR). SmaI restriction site immediately upstream of the FLAG epitope. FLAG epitope in opposite orientation of lac promoter.DepositorTypeEmpty backboneUseSynthetic BiologyTagsFLAG epitope tag (35 bp)ExpressionBacterialMutationPromoterAvailable sinceSept. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHBJT005
Plasmid#225156PurposeLow copy cloning vector for integration of C-terminal FLAG epitope tag (ChlR). SmaI restriction site immediately upstream of the FLAG epitope. FLAG epitope in same orientation as lac promoter.DepositorTypeEmpty backboneUseSynthetic BiologyTagsFLAG epitope tag (35 bp)ExpressionBacterialMutationPromoterAvailable sinceSept. 10, 2024AvailabilityAcademic Institutions and Nonprofits only