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Plasmid#108316PurposeSynthetic RFP based on Corynactis spp under weak synthetic constitutive promoter pFAB4024; kanamycin resistant.DepositorInsertRFP under controlf of weak synthetic constitutive promoter pFAB4024
UseTagsExpressionBacterialMutationPromoterpFAB4024Available sinceAug. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCML 220
Plasmid#98588PurposepCYFP. Expresses CYFP control fusion in E. coli for TriFC assay (kanR).DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterpLacOAvailable sinceSept. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
ReNL-Actin_pcDNA3
Plasmid#89531PurposeRed color variant of bright luminescent protein, fused to actinDepositorInsertReNL
UseTagsActinExpressionMammalianMutationPromoterCMVAvailable sinceMay 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
tubulin-OeNL_pcDNA3
Plasmid#89523PurposeOrange color variant of bright luminescent protein, fused to b-tubulinDepositorInsertOeNL
UseTagsb-tubulinExpressionMammalianMutationPromoterCMVAvailable sinceMay 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
Kohinoor-Fibrilarin/pcDNA3
Plasmid#67781PurposeFibrilarin-targetted Kohinoor for mammalian cell expressionDepositorInsertKohinoor-Fibrilarin
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJRH-1187 VSVGmut
Plasmid#201913PurposeExpresses a VSV-G variant (K47Q R354A) defective for LDL-R receptor binding (CAG promoter)DepositorInsertVSV-G (K47Q, R354A)
UseTagsExpressionMammalianMutationK47Q, R354APromoterCAGAvailable sinceAug. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV CEPIA2mt
Plasmid#58218PurposeGreen fluorescent indicator for calcium imaging in the mitochondriaDepositorInsertCEPIA2mt
UseTagsmycExpressionMammalianMutationGCaMP2 N263Y E282V M339LPromoterCMVAvailable sinceJuly 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBTRCk-pVhb-maACR
Plasmid#215651PurposepBBR1 Origin, KanR, with maACR gene under Vhb promoterDepositorInsertacyl-CoA reductase
UseTagsMaltose binding protein and His tagExpressionBacterialMutationPromoterVhbAvailable sinceJuly 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
CMV-G-GECO1.2
Plasmid#32446PurposeMammalian expression of green fluorescent genetically encoded Ca2+ indicator for optical imaging (version 1.2)DepositorArticleInsertG-GECO1.2
UseTagsExpressionMammalianMutationGCaMP3 K69E/N77Y/D86G/K119I/L173Q/D260G/S404G/E43…PromoterCMVAvailable sinceSept. 29, 2011AvailabilityAcademic Institutions and Nonprofits only -
LAMP-ReNL_pcDNA3
Plasmid#89532PurposeRed color variant of bright luminescent protein, fused to Lysosomal-associated membrane protein 1DepositorInsertReNL
UseTagsLAMP1ExpressionMammalianMutationPromoterCMVAvailable sinceMay 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
CpxR-GFP
Plasmid#220150PurposeTo track the natural CpxR promoter's transcriptional dynamicsDepositorInsertCpxR promoter only
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
p201N Cas9
Plasmid#59175PurposeCas9 driven by double 35S, nptII for plant selection, I-PpoI site to accept gRNA from pUC gRNA ShuttleDepositorInsertsCas9
nptII
UseCRISPRTagsExpressionMutationPromoter2x35S and StUbi-3PAvailable sinceSept. 26, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB304
Plasmid#68632PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneUseTagsGUSExpressionPlantMutationPromoterAvailable sinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDN-Sth1Cas9
Plasmid#221386PurposeCas9 under control of AHT inducible TetR on integrating plasmid (KanR) using L5 integrase and attP for site-specific integration into attBDepositorInsertSth1 Cas9
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJuly 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
CLPIT Cry2PHR-mCh-RhoA
Plasmid#42959DepositorInsertCry2PHR-mCherry-RhoA (RHOA Mustard Weed, Human)
UseRetroviralTagsExpressionMammalianMutationPromoterTet-Off promoterAvailable sinceApril 9, 2013AvailabilityAcademic Institutions and Nonprofits only -
His-CL7-2NLS-SpyCas9(dS)-A22p3star
Plasmid#222970PurposeBacterial expression of A22p-fused SpyCas9 constructDepositorInsertHis-CL7-2NLS-SpyCas9(dS)-A22p3
UseCRISPRTagsCL7 and HisExpressionBacterialMutationPromotertacAvailable sinceJuly 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCpf1b
Plasmid#122187PurposeBacterial genome editing plasmid with kanamycin resistance marker and using sacB as a counter-selection markerDepositorTypeEmpty backboneUseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceMarch 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pX330A-FBL/PITCh
Plasmid#63671PurposeExpresses Cas9 nuclease, FBL-specific gRNA, and the PITCh-gRNADepositorInserthumanized S. pyogenes Cas9
UseCRISPRTags3xFLAGExpressionMammalianMutationPromoterCBhAvailable sinceDec. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
Sec61b-V5-TurboID
Plasmid#166971PurposeExpresses ER targeted TurboID for secretome biotinylationDepositorInsertSec61b-V5-TurboID
UseTagsV5ExpressionMammalianMutationPromoterCMV promoterAvailable sinceApril 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBTK527
Plasmid#110605PurposeBTK Assembled plasmid- ready for Stage 2 assembly (ConLS' and ConRE' connectors with kanamycin resistance)DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 14, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits