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Plasmid#198287PurposeProduces PVCs retargeted with anti-EGFR DARPin E01DepositorInsertPVCpnf_pvc13-E01DARPin
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
HA-GFP1-10-pDEST-C
Plasmid#118369PurposeBimolecular Fluorescence Complimentation (BiFC) assay. Gateway compatible C-terminal vector with BiFC-tag (HA-GFP1-10) to screen interaction partners.DepositorTypeEmpty backboneUseDestinationTagsHA-GFP1-10ExpressionMammalianMutationPromoterAvailable sinceJan. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET30a-HS-Tn5
Plasmid#127916PurposeProkaryotic expression of Hyperstable Tn5 (HS-Tn5) for Illumina library preperation. Developed for the large scale ChIP-Seq and ATAC-Seq experiments for the fruitENCODE and Maize Cistrome projects.DepositorInsertHyperstable Tn5
UseTagsE coli elongation factor Ts and Mxe intein - Chit…ExpressionBacterialMutationE54K, L372PPromoterT7Available sinceJuly 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV-dCas13-M3nls
Plasmid#155366PurposeTargeted m6A RNA methylation in mammalian cellsDepositorInsertdCas13-M3nls (METTL3 Cas13b is from Prevotella sp. P5-125; METTL3 is from H. sapiens)
UseCRISPRTagsExpressionMammalianMutationPspCas13b Δ984-1090 H133APromoterAvailable sinceJuly 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
PB-TO-oNGN2
Plasmid#198397PurposePiggybac Tet-ON plasmid for differentiating hiPSCs into glutamatergic neurons via NGN2 expression. oNGN2 is a Phospho-mutant which avoids inhibition by cdk kinase, NGN2 optimization aided by IDT tool.InsertsUseTagsT2A-mycNLS-mTagBFP2ExpressionMammalianMutationS24A, S193A, S207A, S209A, S219A, S232A, S239A, S…PromoterCAG, EF1a, and TRE3GAvailable sinceApril 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pmiRFP670-N1
Plasmid#79987PurposeMonomeric near-infrared fluorescent protein miRFP670 in mammalian plasmidDepositorHas ServiceCloning Grade DNAInsertmiRFP670
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
HyPer7.2DAAO-NLS
Plasmid#168302PurposeChemogenetic fluorescent H2O2 reporter targeted to the nucleusDepositorInsertHyPer7.2DAAO-NLS
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/TO/Strep-HA-ZAK alpha
Plasmid#141193PurposeExpresses Strep-HA-ZAK alpha in mammalian cells, can be used to make inducible cell lineDepositorInsertMAP3K20 (MAP3K20 Human)
UseTagsStrep, HAExpressionMammalianMutationPromoterCMVAvailable sinceJune 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pNL(CMV)Luc2-Turbo RFP/CMV/WPREdU3
Plasmid#136959PurposeReporter vector encoding firefly luciferase and TurboRFP fluorescent proteinDepositorInsertFirefly luciferase and TurboRFP
UseLentiviralTagsV5 tagExpressionMutationPromoterCMVAvailable sinceFeb. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pcDNA3-HA-mRipk3
Plasmid#78805PurposeTo overexpress mRipk3 in Mammalian CellsDepositorInsertRIPK3 (Ripk3 Mouse)
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceJune 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTK168_mCherry-H2B
Plasmid#46363Purposevisualizes chromosomesDepositorInsertH2B (H2BC11 Human)
UseRetroviralTagsmCherryExpressionMammalianMutationD26G, V119I and S125DPromoterpBABEAvailable sinceAug. 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
3xnls-mNeonGreen
Plasmid#98875PurposeIn vivo visualization of the nucleus (can be used for colocalization studies)DepositorArticleInsert3xNLS
UseTagsmNeonGreenExpressionMammalianMutationPromoterCMVAvailable sinceMarch 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV-AML1-ETO
Plasmid#12428DepositorUseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 17, 2006AvailabilityAcademic Institutions and Nonprofits only -
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AAV.TBG.PI.Cre.rBG
Plasmid#107787PurposeAAV vector expressing Cre from TBG promoterDepositorHas ServiceAAV8InsertCre
UseAAVTagsExpressionMammalianMutationPromoterTBGAvailable sinceMay 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
FKBP-V5-AP-nes_pLX304
Plasmid#120912PurposeLentiviral vector expressing FKBP-V5-AP-NES in mammalian cytosolDepositorInsertFKBP-V5-AP-NES
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHO4d-Cas9
Plasmid#67881PurposeCas9nlsHis6 Bacterial Expression constructDepositorInsertCas9
UseCRISPRTagsHis6 and T7 epitopeExpressionBacterialMutationPromoterT7Available sinceSept. 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
CMV-B-GECO1
Plasmid#32448PurposeMammalian expression of blue fluorescent genetically encoded Ca2+ indicator for optical imagingDepositorArticleInsertB-GECO1.0
UseTagsExpressionMammalianMutationGCaMP3 S40P/L60P/D86G/K119I/L173Q/T223S/Y224H/D30…PromoterCMVAvailable sinceSept. 30, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCFJ782 - HygroR
Plasmid#190933PurposeHygromycin resistance co-injection markerDepositorInsertHygromycin resistance gene
UseTagsExpressionWormMutationPromoterrps-0Available sinceNov. 8, 2022AvailabilityAcademic Institutions and Nonprofits only