Addgene

Showing: 3981 - 4000 of 18501 results

Results per page:

Sort results by:

MCP-APEX2

Plasmid

#154936

Purpose

NLS-V5-MCP-APEX2

Depositor

Alice Ting

Article

Han et al Proc Natl Acad Sci U S A. 2020 Aug 24. p

Insert

NLS-V5-MCP-APEX2

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 4, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
AMPK-γ1-HA

Plasmid

#40605

Depositor

Harvey Lodish

Article

Wong et al J Biol Chem. 2006 Nov 24;281(47):36434-4

Insert

AMPK-γ1 (Prkag1 Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 27, 2012

Availability

Academic Institutions and Nonprofits only
pLPCX-IRES-GFP

Plasmid

#65436

Purpose

For transfection or retroviral production. Mammalian expression of EGFP driven by the ECMV IRES.

Depositor

Trond Aasen

Article

Salat-Canela et al Cell Commun Signal. 2014 May 8;12:31. do

Insert

IRES-GFP

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 11, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
2XMyc-LRRK2-3XKD

Plasmid

#25366

Depositor

Mark Cookson

Article

J Biol Chem. 2008 Jun 13;283(24):16906-1

Insert

LRRK2 (LRRK2 Human)

Use

Tags

2XMyc

Expression

Mammalian

Mutation

"Kinase Dead" Lysine 1906 mutated to A…

Promoter

Available since

June 29, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
pFREE

Plasmid

#92050

Purpose

Allows inducible expression of gRNAs and Cas9 for plasmid curing.

Depositor

Morten Norholm

Article

Lauritsen et al Microb Cell Fact. 2017 Aug 2;16(1):135.

Inserts

Cas9
gRNA array

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

pRhamBAD and ptet

Available since

July 21, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pRC_RR_VP1_r1c3

Plasmid

#65724

Purpose

Rescued infectious variant from pAAV2_RaPID library; mCherry insertion in place of the deleted amino acid sequence 453-GTTTQSR

Depositor

Junghae Suh

Article

Judd et al Mol Ther Nucleic Acids. 2012 Nov 13;1:e5

Insert

mCherry

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

Aug. 17, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pSGKP-km

Plasmid

#117233

Purpose

A sgRNA expression plasmid for genome editing in Klebsiella Pneumoniae

Depositor

Quanjiang Ji

Article

Wang et al Appl Environ Microbiol. 2018 Sep 14. pii

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 7, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
phage UbiC G3BP1-GFP-GFP

Plasmid

#119950

Purpose

Lentiviral vector expressing G3BP1-GFP fusion protein

Depositor

Jeffrey Chao

Article

Wilbertz et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

Insert

G3BP1 (G3BP1 Human)

Use

Lentiviral

Tags

GFP

Expression

Bacterial and Mammalian

Mutation

Promoter

UbiC

Available since

March 20, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pShuttle-CMV

Plasmid

#16403

Purpose

Shuttle vector for use in AdEasy System. Used for expression of transgenes under a CMV promoter when no GFP tracer is desired.

Depositor

Bert Vogelstein

Article

He et al Proc Natl Acad Sci U S A. 1998 Mar 3. 95

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Adenoviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 11, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
pKD236-4b

Plasmid

#90956

Purpose

Expresses ThsS constitutively

Depositor

Jeffrey Tabor

Article

Daeffler et al Mol Syst Biol. 2017 Apr 3;13(4):923. doi

Insert

ThsS

Use

Tags

Expression

Bacterial

Mutation

Promoter

Bba_J23104

Available since

May 16, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
Plenti-CMV-MCS-RFP-SV-puro

Plasmid

#109377

Purpose

This plasmid expresses dsRed2 and puromycin resistance

Depositor

Behzad Gerami-Naini, Jonathan Garlick

Type

Empty backbone

Use

Lentiviral

Tags

dsRed2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 1, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pFL-SV40

Plasmid

#115352

Purpose

firefly luciferase (Fluc) reporter gene driven by SV40 promoter

Depositor

Ming-Chih Lai

Article

Lai et al Mol Biol Cell. 2008 Sep;19(9):3847-58. d

Type

Empty backbone

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 13, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
CAG-LoxpStopLoxp-NLS-dCas9-HA-NLS-NLS-10xGCN4-NLS-P2A-scFv-NLS-P65-HSF1-Flag-T2A-EGFP-WPRE-PolyA

Plasmid

#107307

Purpose

Encoding an SPH activation system

Depositor

Hui Yang

Article

Zhou et al Nat Neurosci. 2018 Jan 15. pii: 10.1038/

Insert

CAG-LSL-dCas9-10xGCN4-P2A-scFv-P65-HSF1-T2A-EGFP-WPRE-PolyA

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 7, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
R777-E011 Hs.ARHGAP35

Plasmid

#70295

Purpose

Gateway ORF clone of human ARHGAP35 [NM_004491.4] with stop codon (for native or N-terminal fusions)

Depositor

Dominic Esposito

Insert

ARHGAP35 (ARHGAP35 Human)

Use

Gateway entry clone

Tags

Expression

Mutation

Promoter

Available since

March 1, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pN1/G3BP1-iRFP

Plasmid

#129339

Purpose

Stress granule marker

Depositor

Yasushi Okada

Article

Yaginuma et al bioRxiv 2021.10.08.463131

Insert

iRFP (from Dr. M. Matsuda)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 21, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pCS2FLAG

Plasmid

#16331

Depositor

Peter Klein

Insert

Flag tag

Use

Vertebrate expression

Tags

Flag

Expression

Mutation

Flag tag inserted into pCS2+ polylinker

Promoter

Available since

Dec. 21, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
pmScarlet3-alphaTubulin_C1

Plasmid

#189768

Purpose

Construct for labelling microtubules in mammalian cells with mScarlet3-alphatubulin

Depositor

Dorus Gadella

Article

Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

Insert

mScarlet3-alphaTubulin

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 21, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pGTag-TagRFP-CAAX-SV40

Plasmid

#117809

Purpose

Donor for precise CRISPR directed genomic integration using the GeneWeld method

Depositor

Jeffrey Essner

Article

Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

Insert

TagRFP

Use

Synthetic Biology

Tags

CAAX

Expression

Mutation

Promoter

Available since

Feb. 4, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pSNAPf-hYTHDF1

Plasmid

#155346

Purpose

Express full-length human YTHDF1 with a C-terminal SNAP-tag in mammalian cells

Depositor

Xiaowei Zhuang

Article

Fu et al Nat Chem Biol. 2020 May 25. pii: 10.1038

Insert

YTHDF1 (YTHDF1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 19, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a-AviTag-SnoopLigase

Plasmid

#105626

Purpose

Plasmid for recombinant bacterial expression of SnoopLigase, a protein that promotes the formation of an isopeptide bond between 2 peptide tags (SnoopTagJr and DogTag), with AviTag for biotinylation

Depositor

Mark Howarth

Article

Buldun et al J Am Chem Soc. 2018 Feb 28;140(8):3008-3

Insert

AviTag-SnoopLigase

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 16, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge