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Plasmid#161881PurposeStandard Gateway (attR1-attR2) vector for expression of maltose-binding protein (MBP) fusions using the Bac-to-Bac insect cell systemDepositorTypeEmpty backboneUseTagsMBPExpressionInsectMutationPromoterAvailable sinceJan. 21, 2021AvailabilityAcademic Institutions and Nonprofits only
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pINDUCER20_3xFlag
Plasmid#190028PurposeDoxycycline-inducible lentiviral vector for expression of ORF with C-terminal 3xFlagDepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceNov. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET32a-DEST
Plasmid#84652PurposeE. coli expression vector derived from pET32 with Gateway cassette.DepositorTypeEmpty backboneUseTagsHis, S tag, and Trx TagExpressionBacterialMutationPromoterT7Available sinceNov. 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
plex_307-EYFP-ccdB-zeo
Plasmid#201129PurposepLEX_307 plasmid with N-terminal EYFP tag and zeo markerDepositorTypeEmpty backboneUseTagsEYFPExpressionMammalianMutationPromoterEF-1αAvailable sinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti6.2-ccdB-Firefly
Plasmid#87074PurposeLentiviral Gateway-compatible expression vector backbone with a C-terminal Firefly luciferaseDepositorTypeEmpty backboneUseLentiviralTagsFirefly luciferaseExpressionMammalianMutationPromoterAvailable sinceMarch 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDest-633
Plasmid#161877PurposeStandard Gateway (attR1-attR2) vector for expression of His6-GST fusions using the Bac-to-Bac insect cell systemDepositorTypeEmpty backboneUseTagsHis6-GSTExpressionInsectMutationPromoterAvailable sinceMarch 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDEST-Spe4-RfA
Plasmid#186375PurposeDestination vector for the expression of a protein of interest into embryos, by mRNA microinjectionDepositorTypeEmpty backboneUseGateway destination vectorTagsExpressionMutationPromoterAvailable sinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
plex_307-EYFP-ccdB-blasticidin
Plasmid#201131PurposepLEX_307 plasmid with N-terminal EYFP tag and blasticidin markerDepositorTypeEmpty backboneUseTagsEYFPExpressionMammalianMutationPromoterEF-1αAvailable sinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCFJ210
Plasmid#30538DepositorInsertttTi4348 region
UseTagsExpressionWormMutationPromoterAvailable sinceJune 29, 2011AvailabilityAcademic Institutions and Nonprofits only -
pLenti6.2-Firefly-ccdB
Plasmid#87077PurposeLentiviral Gateway-compatible expression vector backbone with an N-terminal Firefly luciferaseDepositorTypeEmpty backboneUseLentiviralTagsFirefly luciferaseExpressionMammalianMutationPromoterAvailable sinceApril 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
plex_307-mKate-ccdB-zeo
Plasmid#201126PurposepLEX_307 plasmid with N-terminal mKate tag and zeo markerDepositorTypeEmpty backboneUseTagsmKateExpressionMammalianMutationPromoterEF-1αAvailable sinceJune 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
plex_307-3xflag-ccdb-blasticidin
Plasmid#201123PurposepLEX_307 plasmid with N-terminal FLAG tag and blasticidin markerDepositorTypeEmpty backboneUseTags3xFLAGExpressionMammalianMutationPromoterEF-1αAvailable sinceJune 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUPD2 Etr4 (GB2719)
Plasmid#160602DepositorInsertEtr4
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceJan. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMDC32B-AtTAS1c-D2-B/c
Plasmid#137884PurposePlant expression vector (2x35S) for direct cloning of synthetic trans-acting siRNAs into Arabidopsis thaliana TAS1c precursor into position downstream 3'D1[+]DepositorInsertAtTAS1c-D2-B/c
UseTagsExpressionPlantMutationA. thaliana TAS1c precursor sequence including a …Promoter2x35SAvailable sinceMay 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTUB1-Spy.dCas9-CAT-U6-sgRNA(decoy)
Plasmid#171089PurposeCRISPR interference. Construct for expressing catalytically inactive Cas9 (dCas9) from Streptococcus pyogenes in Toxoplasma gondii. Suitable for generating stable cell lines.DepositorInsertsDecoy sgRNA
dCas9
Chloramphenicol acetyltransferase
UseCRISPR; Toxoplasma gondii expressionTags3X FLAG and Nuclear Localization SignalExpressionMutationD10A, H840APromoterTUB1 (Toxoplasma gondii) and U6 (Toxoplasma gondi…Available sinceOct. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pH7WG2B-OsMIR390-B/c
Plasmid#61465PurposePlant expression vector (OsUBI promoter) for direct cloning of artificial microRNAs into rice MIR390 precursor. To use for expressing amiRNAs in monocots.DepositorInsertOsMIR390-B/c
UseTagsExpressionPlantMutationO. sativa MIR390 precursor sequence including a c…PromoterOsUBIAvailable sinceMarch 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMDC32B-OsMIR390-B/c
Plasmid#61467PurposePlant expression vector (2x35S) for direct cloning of artificial microRNAs into rice MIR390 precursor. To use for expressing amiRNAs in monocots.DepositorInsertOsMIR390-B/c
UseTagsExpressionPlantMutationO. sativa MIR390 precursor sequence including a c…Promoter2x35SAvailable sinceMarch 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pENTR-AtTAS1c-D2-B/c
Plasmid#137883PurposeGateway-compatible entry vector for direct cloning of synthetic trans-acting siRNAs into Arabidopsis thaliana TAS1c precursor at position downstream 3'D1[+]DepositorInsertAtTAS1c-D2-B/c
UseGateway-compatible entry cloneTagsExpressionMutationA. thaliana TAS1c precursor sequence including a …PromoterAvailable sinceMay 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMDC123SB-OsMIR390-B/c
Plasmid#61466PurposePlant expression vector (2x35S) for direct cloning of artificial microRNAs into rice MIR390 precursor. To use for expressing amiRNAs in monocots.DepositorInsertOsMIR390-B/c
UseTagsExpressionPlantMutationO. sativa MIR390 precursor sequence including a c…Promoter2x35SAvailable sinceMarch 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDest-527
Plasmid#11518PurposeGateway destination vector for bacterial expression; His tagDepositorTypeEmpty backboneUseGateway destination vectorTagsHis6ExpressionBacterialMutationPromoterAvailable sinceMay 24, 2006AvailabilityAcademic Institutions and Nonprofits only