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Plasmid#125838PurposeKO BAP1 geneDepositorInsertBAP1 (BRCA1 associated protein 1) (BAP1 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJune 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBIG1b_nsp7 (SARS-CoV-2)
Plasmid#169178PurposeBaculoviral transfer vector to express untagged nsp7 (SARS-CoV-2) in insect cellsDepositorInsertnsp7 (ORF1ab Synthetic, SARS-CoV-2)
UseTagsuntaggedExpressionInsectMutationCodon optimised for insect cells (Spodoptera frug…PromoterPolyhedrinAvailable sinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBIG1c_nsp8 (SARS-CoV-2)
Plasmid#169179PurposeBaculoviral transfer vector to express untagged nsp8 (SARS-CoV-2) in insect cellsDepositorInsertnsp8 (ORF1ab Synthetic, SARS-CoV-2)
UseTagsuntaggedExpressionInsectMutationCodon optimised for insect cells (Spodoptera frug…PromoterPolyhedrinAvailable sinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pmCellFree_KA1_SARS-COV-2_RBD -N501Y
Plasmid#169407PurposeCell-free/mammalian expression vector for SARS-COV-2 mutated RBD domain (N510Y, UK strain) of S protein with N-term eGFPDepositorInsertSARS-COV-2 _RBD (N501Y) (S SARS-Cov2)
UseCell-free expression vectorTagsHis and eGFPExpressionMutationN501YPromoterT7- CMV/CAGAvailable sinceJune 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
px335 Mettl14 sgRNA #2
Plasmid#61514Purposeencodes sgRNA sequence for targeting mouse Mettl14 locus (Cas9-Nickase strategy)DepositorInsertgccgctcccggatctcctgc
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJan. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmTRF4-2
Plasmid#66204Purposeexpresses EGFP-tagged TRF4-2 in Drosophila cellsDepositorInsertTRF4-2 (Trf4-2 Fly)
UseDrosophila expressionTagsEGFPExpressionMutationPromoterActin 5CAvailable sinceJune 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAD48-daf-2 RNAi
Plasmid#34834DepositorInsertdaf-2 (daf-2 Nematode)
UseRNAiTagsExpressionBacterialMutationPromoterAvailable sinceJune 11, 2012AvailabilityAcademic Institutions and Nonprofits only -
pH2B-miRFP670-2-N1
Plasmid#197228PurposeExpresses the fusion protein of H2B-miRFP670-2 in mammalian cellsDepositorInsertH2B-miRFP670-2
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pKeratin-miRFP670-2-N1
Plasmid#197233PurposeExpresses the fusion protein of Keratin-miRFP670-2 in mammalian cellsDepositorInsertKeratin-miRFP670-2
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCytERM-miRFP670-2-N1
Plasmid#197181PurposeExpresses the fusion protein of CytERM-miRFP670-2 in mammalian cellsDepositorInsertCytERM-miRFP670-2
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-miRFP670-2
Plasmid#197201PurposeExpresses the protein of miRFP670-2 in mammalian cellsDepositorInsertmiRFP670-2
UseAAVTagsExpressionMutationPromoterAvailable sinceMarch 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJM601-TRA-2::FLAG
Plasmid#27129DepositorInsertTRA-2 FLAG (tra-2 Nematode)
UseTags3x FLAGExpressionWormMutationReplaced TRA-2 stop codon with KpnI and EcoRI and…PromoterAvailable sinceFeb. 16, 2011AvailabilityAcademic Institutions and Nonprofits only -
pJM614-TRA-2::GFP
Plasmid#27128DepositorInsertTRA-2 GFP (tra-2 Nematode)
UseTagsGFPExpressionWormMutationreplaced stop codon with AgeI and EcoRI added GFP…PromoterAvailable sinceJan. 5, 2011AvailabilityAcademic Institutions and Nonprofits only -
pEF1α -NWM-Node 2
Plasmid#154254Purposeexpresses reconstructed New World monkey retroCHMP3 Node 2 in mammalian cellsDepositorInsertreconstructed Node 2 of New World monkey retroCHMP3
UseTagsHAExpressionMammalianMutationPromoterEF1-alphaAvailable sinceApril 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
GAL4-mNFAT1(2-144)
Plasmid#11108PurposeExpresses mouse NFAT1 (amino acids 2-144) fused to GAL4 DNA binding domain in mammalian cellsDepositorInsertNFAT1 (Nfatc2 Mouse)
UseTagsGAL4-DBDExpressionMammalianMutationContains NFAT1 amino acids 2-144; L78P and P140Q …PromoterAvailable sinceApril 24, 2006AvailabilityAcademic Institutions and Nonprofits only -
pMRXIP-GFP-Evectin-2
Plasmid#221754PurposeStable expression of Evectin-2 in mammalian cellsDepositorInsertPLEKHB2 (PLEKHB2 Human)
UseRetroviralTagsGFPExpressionMammalianMutationtandem PLEKHB2(PH domain) (amino acids 1-110)PromoterAvailable sinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPR v2-sgDHODH-2
Plasmid#186023Purposeknock out DHODH in mammalian cellsDepositorInsertDihydroorotate Dehydrogenase (Quinone) (DHODH Human)
UseCRISPR and LentiviralTagsExpressionMutationPromoterU6Available sinceJune 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPR v2 sgKEAP1-2
Plasmid#186459Purposeknock out KEAP1 in mammalian cellsDepositorInsertKeap1 (Kelch-like ECH-associated protein 1) (KEAP1 Human)
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceSept. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
mCerulean3-Bcl-2-s2193
Plasmid#166750PurposeExpress mCerulean3 fused to the N-terminus of the Bcl-2 family protein, Bcl-2 in a plasmid with Blasticidin resistance. Used to make stable BMK-DKO cell lines.DepositorInsertBcl-2 (BCL2 Human)
UseTagsmCerulean3ExpressionMammalianMutationPromoterHuman ferritinAvailable sinceOct. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV6-AN-DDK_UBM1*2* POLI
Plasmid#131232PurposeMammalian expression of an N-terminally FLAG-tagged DNA polymerase iota mutant, containing point mutations in both UBM domainsDepositorInsertDNA polymerase iota (POLI Human)
UseTagsFLAGExpressionMammalianMutationContains inactivating mutations in both ubiquitin…PromoterCMV enhancer + CMV promoterAvailable sinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only