Skip to main content
Addgene
Showing: 951 - 1000 of 14026 results

  19. pTRE3G-mouse cGAS:145-508aa-HA

    Plasmid
    #186895
    Purpose
    Doxycycline-dependent expression of mouse cGAS gene in mammalian cells by retroviral transduction
    Depositor
    Jonathan Kagan
    Article
    Mosallanejad et al Sci Immunol. 2023 Jan 20;8(79):eabp9765.
    Insert
    Mouse cGAS truncation mutant (145-508 a.a.) with C-terminal HA tag (Cgas Mouse, Synthetic)
    Use
    Retroviral
    Tags
    HA
    Expression
    Mutation
    N-terminal truncation
    Promoter
    TRE3G
    Available since
    Oct. 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    186895-plasmid-map-sequence-id-366530

    Enlarge

  20. pTetOne-human cGASdeltaN-HA

    Plasmid
    #186897
    Purpose
    Doxycycline-dependent expression of human cGAS gene in mammalian cells by retroviral transduction
    Depositor
    Jonathan Kagan
    Article
    Mosallanejad et al Sci Immunol. 2023 Jan 20;8(79):eabp9765.
    Insert
    Human cGAS truncation mutant (160-522 a.a.) with C-terminal HA tag (Adamts1 Human, Synthetic)
    Use
    Retroviral
    Tags
    HA
    Expression
    Mutation
    N-terminal truncation
    Promoter
    TRE3G
    Available since
    Oct. 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    186897-plasmid-map-sequence-id-366541

    Enlarge

  21. pTetOne-orangutan cGASdeltaN-HA

    Plasmid
    #186898
    Purpose
    Doxycycline-dependent expression of human cGAS gene in mammalian cells by retroviral transduction
    Depositor
    Jonathan Kagan
    Article
    Mosallanejad et al Sci Immunol. 2023 Jan 20;8(79):eabp9765.
    Insert
    Orangutan cGAS truncation mutant (158-521 a.a.) with C-terminal HA tag (CGAS Pongo abelii, Synthetic)
    Use
    Retroviral
    Tags
    HA
    Expression
    Mutation
    N-terminal truncation
    Promoter
    TRE3G
    Available since
    Oct. 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    186898-plasmid-map-sequence-id-366562

    Enlarge

  22. pTRE3G-chimpanzee cGASdeltaN-HA

    Plasmid
    #186874
    Purpose
    Doxycycline-dependent expression of chimpanzee cGAS gene in mammalian cells by retroviral transduction
    Depositor
    Jonathan Kagan
    Article
    Mosallanejad et al Sci Immunol. 2023 Jan 20;8(79):eabp9765.
    Insert
    Chimpanzee cGAS truncation mutant (160-520 a.a.) with C-terminal HA tag (CGAS Pan troglodytes, Synthetic)
    Use
    Retroviral
    Tags
    HA
    Expression
    Mutation
    N-terminal truncation
    Promoter
    TRE3G
    Available since
    Oct. 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    186874-plasmid-map-sequence-id-366080

    Enlarge

  23. pTRE3G-rhesus macaque cGASdeltaN-HA

    Plasmid
    #186866
    Purpose
    Doxycycline-dependent expression of rhesus macaque cGAS gene in mammalian cells by retroviral transduction
    Depositor
    Jonathan Kagan
    Article
    Mosallanejad et al Sci Immunol. 2023 Jan 20;8(79):eabp9765.
    Insert
    Rhesus macaque cGAS truncation mutant (160-522 a.a.) with C-terminal HA tag (CGAS Macaca mulatta, Synthetic)
    Use
    Retroviral
    Tags
    HA
    Expression
    Mutation
    N-terminal truncation
    Promoter
    TRE3G
    Available since
    Oct. 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    186866-plasmid-map-sequence-id-366074

    Enlarge

  25. pTRE3G-orangutan cGASdeltaN-HA

    Plasmid
    #186872
    Purpose
    Doxycycline-dependent expression of orangutan cGAS gene in mammalian cells by retroviral transduction
    Depositor
    Jonathan Kagan
    Article
    Mosallanejad et al Sci Immunol. 2023 Jan 20;8(79):eabp9765.
    Insert
    Orangutan cGAS truncation mutant (158-521 a.a.) with C-terminal HA tag (CGAS Pongo abelii, Synthetic)
    Use
    Retroviral
    Tags
    HA
    Expression
    Mutation
    N-terminal truncation
    Promoter
    TRE3G
    Available since
    Oct. 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    186872-plasmid-map-sequence-id-366079

    Enlarge

  26. pTRE3G-human cGASdeltaN-HA

    Plasmid
    #186843
    Purpose
    Doxycycline-dependent expression of human cGAS gene in mammalian cells by retroviral transduction
    Depositor
    Jonathan Kagan
    Article
    Mosallanejad et al Sci Immunol. 2023 Jan 20;8(79):eabp9765.
    Insert
    Human cGAS truncation mutant (160-522 a.a.) with C-terminal HA tag (Adamts1 Human, Synthetic)
    Use
    Retroviral
    Tags
    HA
    Expression
    Mutation
    N-terminal truncation
    Promoter
    TRE3G
    Available since
    Oct. 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    186843-plasmid-map-sequence-id-366029

    Enlarge

  27. pTRE3G-orangutan cGASdeltaN-GFP

    Plasmid
    #186846
    Purpose
    Doxycycline-dependent expression of orangutan cGAS gene in mammalian cells by retroviral transduction
    Depositor
    Jonathan Kagan
    Article
    Mosallanejad et al Sci Immunol. 2023 Jan 20;8(79):eabp9765.
    Insert
    Orangutan cGAS truncation mutant (158-521 a.a.) with C-terminal GFP tag (CGAS Pongo abelii, Synthetic)
    Use
    Retroviral
    Tags
    GFP
    Expression
    Mutation
    N-terminal truncation
    Promoter
    TRE3G
    Available since
    Oct. 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    186846-plasmid-map-sequence-id-366035

    Enlarge

  28. pTRE3G-chimpanzee cGASdeltaN-GFP

    Plasmid
    #186847
    Purpose
    Doxycycline-dependent expression of chimpanzee cGAS gene in mammalian cells by retroviral transduction
    Depositor
    Jonathan Kagan
    Article
    Mosallanejad et al Sci Immunol. 2023 Jan 20;8(79):eabp9765.
    Insert
    Chimpanzee cGAS truncation mutant (160-520 a.a.) with C-terminal GFP tag (CGAS Pan troglodytes, Synthetic)
    Use
    Retroviral
    Tags
    GFP
    Expression
    Mutation
    N-terminal truncation
    Promoter
    TRE3G
    Available since
    Oct. 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    186847-plasmid-map-sequence-id-366036

    Enlarge

  29. pTRE3G-HA-human cGASdeltaN

    Plasmid
    #186852
    Purpose
    Doxycycline-dependent expression of human cGAS gene in mammalian cells by retroviral transduction
    Depositor
    Jonathan Kagan
    Article
    Mosallanejad et al Sci Immunol. 2023 Jan 20;8(79):eabp9765.
    Insert
    Human cGAS truncation mutant (160-522 a.a.) with N-terminal HA tag (Adamts1 Human, Synthetic)
    Use
    Retroviral
    Tags
    HA
    Expression
    Mutation
    N-terminal truncation
    Promoter
    TRE3G
    Available since
    Oct. 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    186852-plasmid-map-sequence-id-366437

    Enlarge

  30. pTRE3G-human cGASdeltaN

    Plasmid
    #186854
    Purpose
    Doxycycline-dependent expression of human cGAS gene in mammalian cells by retroviral transduction
    Depositor
    Jonathan Kagan
    Article
    Mosallanejad et al Sci Immunol. 2023 Jan 20;8(79):eabp9765.
    Insert
    Human cGAS truncation mutant (160-522 a.a.) without tags (Adamts1 Human, Synthetic)
    Use
    Retroviral
    Tags
    Expression
    Mutation
    N-terminal truncation
    Promoter
    TRE3G
    Available since
    Oct. 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    186854-plasmid-map-sequence-id-366042

    Enlarge

  32. AiP15381: pAAV-AiE0873m_3xC2-minBG-SYFP2-P2A-mScarlet-10aa-H2B-WPRE3-BGHpA (Alias: CN5381)

    Plasmid
    #230874
    Purpose
    AiE0873m_3xC2 is an enhancer sequence, designed to drive AAV-mediated transgene expression in striatal cholinergic interneurons
    Depositor
    Jonathan Ting, The Allen Institute for Brain Science
    Article
    Hunker et al bioRxiv [Preprint]. 2024 Sep 29:2024.09.
    Insert
    SYFP2-P2A-mScarlet-10aa-H2B
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 29, 2025
    Availability
    Academic Institutions and Nonprofits only
    230874-plasmid-map-sequence-id-459520

    Enlarge

  33. AiP14210: pAAV-AiE0779m_3xC2-minBG-SYFP2-P2A-mScarlet-10aa-H2B-WPRE3-BGHpA (Alias: CN4210)

    Plasmid
    #230868
    Purpose
    AiE0779m_3xC2 is an enhancer sequence, designed to drive AAV-mediated transgene expression in striatal direct pathway MSNs (D1-MSNs)
    Depositor
    Jonathan Ting, The Allen Institute for Brain Science
    Article
    Hunker et al bioRxiv [Preprint]. 2024 Sep 29:2024.09.
    Insert
    SYFP2-P2A-mScarlet-10aa-H2B
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 29, 2025
    Availability
    Academic Institutions and Nonprofits only
    230868-plasmid-map-sequence-id-459487

    Enlarge

  34. AiP13640: pAAV-AiE0949h-minBG-SYFP2-P2A-3XFLAG-10aa-H2B-WPRE3-BGHpA (Alias: CN3640)

    Plasmid
    #230864
    Purpose
    AiE0949h is an enhancer sequence, designed to drive AAV-mediated transgene expression in striatal neuron subset
    Depositor
    Jonathan Ting, The Allen Institute for Brain Science
    Article
    Hunker et al bioRxiv [Preprint]. 2024 Sep 29:2024.09.
    Insert
    SYFP2-P2A-3XFLAG-10aa-H2B
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 29, 2025
    Availability
    Academic Institutions and Nonprofits only
    230864-plasmid-map-sequence-id-459477

    Enlarge

  36. pcDNA-dCas9-FLp300

    Plasmid
    #61356
    Purpose
    encodes S. pyogenes dCas9 with c-terminal fusion of FL human p300 (aa 2-2414) driven by CMV promoter
    Depositor
    Charles Gersbach
    Article
    Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do
    Insert
    S.pyogenes dCas9 with c-terminal full length human p300 (aa 2-2414) (EP300 Human, S. pyogenes, Synthetic)
    Use
    CRISPR and Synthetic Biology
    Tags
    Flag, HA, and SV40 NLS
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9
    Promoter
    CMV
    Available since
    April 16, 2015
    Availability
    Academic Institutions and Nonprofits only
    61356-plasmid-map-sequence-id-99784

    Enlarge

  38. pcDNA3.3-Nm-dCas9-p300 Core

    Plasmid
    #61365
    Purpose
    encodes N. meningiditis dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664) driven by CMV promoter
    Depositor
    Charles Gersbach
    Article
    Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do
    Insert
    Nm-dCas9-p300 Core (EP300 Human, Neisseria meningiditis, Synthetic)
    Use
    CRISPR and Synthetic Biology
    Tags
    HA tag and NLS
    Expression
    Mammalian
    Mutation
    D16A, D587A, H588A, N611A in Nm Cas9
    Promoter
    CMV
    Available since
    April 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    61365-plasmid-map-sequence-id-244292

    Enlarge

  39. pDEST-N2H-F2-empty vector

    Plasmid
    #125552
    Purpose
    N2H assay vector with nanoluc fragment2 (empty control, no Gateway cloning site, expressing nanoluc fragment2)
    Depositor
    Marc Vidal
    Article
    Choi et al Nat Commun. 2019 Aug 29;10(1):3907. doi:
    Type
    Empty backbone
    Use
    In vitro expression
    Tags
    NanoLuc fragment2 alone without any attached prot…
    Expression
    Mammalian and Yeast
    Mutation
    Promoter
    synthetic promoter containing yeast, mammalian, a…
    Available since
    Aug. 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    125552-plasmid-map-sequence-id-252313

    Enlarge

  40. pDEST-N2H-F1-empty vector

    Plasmid
    #125551
    Purpose
    N2H assay vector with nanoluc fragment1 (empty control, no Gateway cloning site, expressing nanoluc fragment1)
    Depositor
    Marc Vidal
    Article
    Choi et al Nat Commun. 2019 Aug 29;10(1):3907. doi:
    Type
    Empty backbone
    Use
    In vitro expression
    Tags
    NanoLuc fragment1 alone without any attached prot…
    Expression
    Mammalian and Yeast
    Mutation
    Promoter
    synthetic promoter containing yeast, mammalian, a…
    Available since
    Aug. 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    125551-plasmid-map-sequence-id-252311

    Enlarge

  41. CROPseq-MPSV-eBFP2

    Plasmid
    #220472
    Purpose
    Expresses either functional CRISPR gRNAs from the hU6-promotor and empowers pairing of CRISPR screens with 3' and 5' scRNA-Seq capturing by FACS-sorting on eBFP2+ without prior resistance selection
    Depositor
    Christian Krebs
    Article
    Enk et al Sci Immunol. 2024 Jun 14;9(96):eadd6774.
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    220472-plasmid-map-sequence-id-437895

    Enlarge

  42. pTRE3G-human cGASdeltaN R255E-HA

    Plasmid
    #186894
    Purpose
    Doxycycline-dependent expression of human cGAS gene (with R255E mutation) in mammalian cells by retroviral transduction
    Depositor
    Jonathan Kagan
    Article
    Mosallanejad et al Sci Immunol. 2023 Jan 20;8(79):eabp9765.
    Insert
    Human cGAS truncation mutant (160-522 a.a.) with R255E mutation and C-terminal HA tag (Adamts1 Human, Synthetic)
    Use
    Retroviral
    Tags
    HA
    Expression
    Mutation
    N-terminal truncation, R255E
    Promoter
    TRE3G
    Available since
    Oct. 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    186894-plasmid-map-sequence-id-366485

    Enlarge

  43. pTetOne-FLAG-human cGASdeltaN-HA

    Plasmid
    #186899
    Purpose
    Doxycycline-dependent expression of human cGAS gene in mammalian cells by retroviral transduction
    Depositor
    Jonathan Kagan
    Article
    Mosallanejad et al Sci Immunol. 2023 Jan 20;8(79):eabp9765.
    Insert
    Human cGAS truncation mutant (160-522 a.a.) with N-terminal FLAG and C-terminal HA tag (Adamts1 Human, Synthetic)
    Use
    Retroviral
    Tags
    FLAG and HA
    Expression
    Mutation
    N-terminal truncation
    Promoter
    TRE3G
    Available since
    Oct. 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    186899-plasmid-map-sequence-id-366534

    Enlarge

  44. pTRE3G-mouse cGASdeltaN-GFP

    Plasmid
    #186878
    Purpose
    Doxycycline-dependent expression of mouse cGAS gene in mammalian cells by retroviral transduction
    Depositor
    Jonathan Kagan
    Article
    Mosallanejad et al Sci Immunol. 2023 Jan 20;8(79):eabp9765.
    Insert
    Mouse cGAS truncation mutant (148-508 a.a.) with C-terminal GFP fusion (Cgas Mouse, Synthetic)
    Use
    Retroviral
    Tags
    GFP
    Expression
    Mutation
    N-terminal truncation
    Promoter
    TRE3G
    Available since
    Oct. 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    186878-plasmid-map-sequence-id-366111

    Enlarge

  45. pTRE3G-FLAG-human cGASdeltaN-GFP

    Plasmid
    #186879
    Purpose
    Doxycycline-dependent expression of human cGAS gene in mammalian cells by retroviral transduction
    Depositor
    Jonathan Kagan
    Article
    Mosallanejad et al Sci Immunol. 2023 Jan 20;8(79):eabp9765.
    Insert
    Human cGAS truncation mutant (160-522 a.a.) with N-terminal FLAG and C-terminal GFP fusion (Adamts1 Human, Synthetic)
    Use
    Retroviral
    Tags
    FLAG and GFP
    Expression
    Mutation
    N-terminal truncation
    Promoter
    TRE3G
    Available since
    Oct. 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    186879-plasmid-map-sequence-id-366112

    Enlarge

  46. pTRE3G-GFP-human cGASdeltaN

    Plasmid
    #186880
    Purpose
    Doxycycline-dependent expression of human cGAS gene in mammalian cells by retroviral transduction
    Depositor
    Jonathan Kagan
    Article
    Mosallanejad et al Sci Immunol. 2023 Jan 20;8(79):eabp9765.
    Insert
    Human cGAS truncation mutant (160-522 a.a.) with N-terminal GFP fusion (Adamts1 Human, Synthetic)
    Use
    Retroviral
    Tags
    GFP
    Expression
    Mutation
    N-terminal truncation
    Promoter
    TRE3G
    Available since
    Oct. 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    186880-plasmid-map-sequence-id-366158

    Enlarge

  47. pTRE3G-human cGASdeltaN-OMM

    Plasmid
    #186881
    Purpose
    Doxycycline-dependent expression of human cGAS gene in mammalian cells by retroviral transduction
    Depositor
    Jonathan Kagan
    Article
    Mosallanejad et al Sci Immunol. 2023 Jan 20;8(79):eabp9765.
    Insert
    Human cGAS truncation mutant (160-522 a.a.) with C-terminal MAVS transmembrane domain (503-541 a.a.) fusion (Adamts1 Human, Synthetic)
    Use
    Retroviral
    Tags
    Expression
    Mutation
    N-terminal truncation, MAVS transmembrane domain …
    Promoter
    TRE3G
    Available since
    Oct. 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    186881-plasmid-map-sequence-id-366160

    Enlarge

  48. pTRE3G-human cGASdeltaN-Pex

    Plasmid
    #186882
    Purpose
    Doxycycline-dependent expression of human cGAS gene in mammalian cells by retroviral transduction
    Depositor
    Jonathan Kagan
    Article
    Mosallanejad et al Sci Immunol. 2023 Jan 20;8(79):eabp9765.
    Insert
    Human cGAS truncation mutant (160-522 a.a.) with C-terminal PEX13 transmembrane domain (136-233 a.a.) fusion (Adamts1 Human, Synthetic)
    Use
    Retroviral
    Tags
    Expression
    Mutation
    N-terminal truncation, PEX13 transmembrane domain…
    Promoter
    TRE3G
    Available since
    Oct. 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    186882-plasmid-map-sequence-id-366440

    Enlarge

  49. pTRE3G-human cGASdeltaN-ER

    Plasmid
    #186883
    Purpose
    Doxycycline-dependent expression of human cGAS gene in mammalian cells by retroviral transduction
    Depositor
    Jonathan Kagan
    Article
    Mosallanejad et al Sci Immunol. 2023 Jan 20;8(79):eabp9765.
    Insert
    Human cGAS truncation mutant (160-522 a.a.) with C-terminal VAMP2 transmembrane domain (86-119 a.a.) fusion (Adamts1 Human, Synthetic)
    Use
    Retroviral
    Tags
    Expression
    Mutation
    N-terminal truncation, VAMP2 transmembrane domain…
    Promoter
    TRE3G
    Available since
    Oct. 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    186883-plasmid-map-sequence-id-366441

    Enlarge

  50. pTRE3G-FLAG-human cGAS N-T2A-deltaN-HA

    Plasmid
    #186886
    Purpose
    Doxycycline-dependent expression of human cGAS gene in mammalian cells by retroviral transduction
    Depositor
    Jonathan Kagan
    Article
    Mosallanejad et al Sci Immunol. 2023 Jan 20;8(79):eabp9765.
    Insert
    Human cGAS with T2A ribosome skipping sequences between N-terminus (1-159 a.a.) and C-terminus (160-522 a.a.) (Adamts1 Human)
    Use
    Retroviral
    Tags
    FLAG and HA
    Expression
    Mutation
    Insertion of T2A ribosome skipping site
    Promoter
    TRE3G
    Available since
    Oct. 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    186886-plasmid-map-sequence-id-366445

    Enlarge

Showing: 951 - 1000 of 14026 results