SS32 pComb3xss anti-SAPAP2 nanobody
(Plasmid
#145822)
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PurposeBacterial expression of anti-SAPAP2 (Rat) recombinant llama nanobody for use as an immunolabel
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Depositing Lab
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Sequence Information
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Sequences (2) — Accept Affinity Reagent Sequence Policy
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Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 145822 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepComb3xss
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameanti-SAPAP2 (Rat) recombinant llama nanobody SS32
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SpeciesL. glama (llama)
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Entrez GeneDlgap2 (a.k.a. DAP-2, SAPAP2)
- Promoter lac
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Tag
/ Fusion Protein
- 6xHis-HA (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site SfiI (not destroyed)
- 3′ cloning site SfiI (not destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
SS32 pComb3xss anti-SAPAP2 nanobody was a gift from James Trimmer (Addgene plasmid # 145822 ; http://n2t.net/addgene:145822 ; RRID:Addgene_145822) -
For your References section:
A toolbox of nanobodies developed and validated for use as intrabodies and nanoscale immunolabels in brain neurons. Dong JX, Lee Y, Kirmiz M, Palacio S, Dumitras C, Moreno CM, Sando R, Santana LF, Sudhof TC, Gong B, Murray KD, Trimmer JS. Elife. 2019 Sep 30;8. pii: 48750. doi: 10.7554/eLife.48750. 10.7554/eLife.48750 PubMed 31566565