Addgene

We narrowed to 2,257 results for: PRK

Showing: 571 - 600 of 2257 results

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pRK5 Flag-RagC(S75N)

Plasmid

#99724

Purpose

Mammalian expression of Flag-RagC(S75N)

Depositor

David Sabatini, Kuang Shen

Article

Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

Insert

RagC (RRAGC )

Use

Tags

Flag

Expression

Mutation

S75N

Promoter

CMV

Available since

Nov. 3, 2017

Availability

Academic Institutions and Nonprofits only

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pRK5-mEGFP-HMGB1-WT-IDR

Plasmid

#194549

Purpose

Mammalian Expression of mEGFP-HMGB1 WT IDR Fusion Protein

Depositor

Denes Hnisz

Article

Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

Insert

HMGB1 (HMGB1 Human)

Use

Tags

mEGFP

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pRK5-mEGFP-PHOX2B-MUT

Plasmid

#194577

Purpose

Mammalian Expression of mEGFP-PHOX2B Mutant (S207Afs*102) Fusion Protein

Depositor

Denes Hnisz

Article

Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

Insert

PHOX2B (PHOX2B Human)

Use

Tags

mEGFP

Expression

Mammalian

Mutation

S207Afs*102

Promoter

Available since

Feb. 22, 2023

Availability

Academic Institutions and Nonprofits only

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GABA BR2-flag/pRK5

Plasmid

#197323

Purpose

Plasmid expressing an antigen targeting the GABA(B)R2 metabotropic GABA receptor

Depositor

Stephen Moss

Insert

GABA B R2 (Gabbr2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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[pSK600] pRK5 HA-Nprl2(R78A)

Plasmid

#136146

Purpose

mammalian expression of Nprl2

Depositor

David Sabatini, Kuang Shen

Article

Shen et al J Biol Chem. 2019 Feb 22;294(8):2970-297

Insert

Nprl2 (NPRL2 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

R78A

Promoter

Available since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pRK7-HA-S6K1-E389-deltaCT

Plasmid

#8993

Depositor

John Blenis

Article

Schalm et al Curr Biol. 2002 Apr 16. 12(8):632-9.

Insert

S6K1 (Rps6kb1 Rat)

Use

Tags

HA

Expression

Mammalian

Mutation

T389E, stop codon at amino acid 402

Promoter

Available since

Dec. 7, 2005

Availability

Academic Institutions and Nonprofits only

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pRK5-myc-Cdc42-Q61L

Plasmid

#12974

Depositor

Gary Bokoch

Insert

Cdc42 constitutively active (CDC42 Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

Q61L Constitutively active

Promoter

Available since

March 2, 2007

Availability

Academic Institutions and Nonprofits only

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pRK5-myc-Cdc42-T17N

Plasmid

#12973

Depositor

Gary Bokoch

Insert

Cdc42 DN (CDC42 Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

T17N Dominant negative

Promoter

Available since

Oct. 27, 2006

Availability

Academic Institutions and Nonprofits only

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pFE-Rrub-FII-prk

Plasmid

#162695

Purpose

Expresses R. rubrum Form II rubisco and S. elongatus Prk

Depositor

David Savage

Article

Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1

Inserts

Rubrum Form II rubisco
S. elongatus PRK

Use

Tags

Expression

Bacterial

Mutation

Promoter

PLtet0-1 promoter and under same promoter

Available since

Feb. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pRK5-HA GST RagB 99L

Plasmid

#19303

Depositor

David Sabatini

Article

Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

Insert

Ras-related GTP binding B (RRAGB Human)

Use

Tags

GST and HA

Expression

Mammalian

Mutation

Q99L

Promoter

Available since

Sept. 15, 2008

Availability

Academic Institutions and Nonprofits only

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pRK5-mEGFP-HMGB3-MUT

Plasmid

#194565

Purpose

Mammalian Expression of mEGFP-HMGB3 Mutant (K161Ifs*55) Fusion Protein

Depositor

Denes Hnisz

Article

Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

Insert

HMGB3 (HMGB3 Human)

Use

Tags

mEGFP

Expression

Mammalian

Mutation

K161Ifs*55

Promoter

Available since

Feb. 22, 2023

Availability

Academic Institutions and Nonprofits only

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PBAD-his6-prkA-pACYC184

Plasmid

#41041

Depositor

Ichiro Matsumura

Article

Parikh et al Protein Eng Des Sel. 2006 Mar;19(3):113-

Insert

prkA

Use

Tags

6xHis and T7 epitope tag

Expression

Bacterial

Mutation

Promoter

PBAD

Available since

Nov. 6, 2012

Availability

Academic Institutions and Nonprofits only

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pRK5-HA-GST-4EBP1-Low GC

Plasmid

#69018

Purpose

expresses tagged 4EBP1

Depositor

David Sabatini

Article

Grabiner et al Cancer Discov. 2014 May;4(5):554-63. doi

Insert

EIF4EBP1 (EIF4EBP1 Human)

Use

Tags

GST and HA

Expression

Mammalian

Mutation

lowGC content, wt is ~70% GC rich. Still, it code…

Promoter

Available since

Jan. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pRK5-Myc-TEAD1-Y406A

Plasmid

#33047

Depositor

Kunliang Guan

Article

Li et al Genes Dev. 2010 Feb 1;24(3):235-40.

Insert

TEAD1 (TEAD1 Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

Y406A

Promoter

Available since

Feb. 10, 2012

Availability

Academic Institutions and Nonprofits only
pRK5-HA GST RagC 75L

Plasmid

#19305

Depositor

David Sabatini

Article

Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

Insert

Ras-related GTP binding C (RRAGC Human)

Use

Tags

GST and HA

Expression

Mammalian

Mutation

S75L

Promoter

Available since

Sept. 15, 2008

Availability

Academic Institutions and Nonprofits only

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pRK5-EGFP-Tau AP P301L

Plasmid

#46906

Purpose

expresses EGFP tagged Tau AP P301L in mammalian cells

Depositor

Karen Ashe

Article

Hoover et al Neuron. 2010 Dec 22;68(6):1067-81. doi:

Insert

Tau AP P301L (MAPT Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

P301L AND all 14 S/P or T/P amino acid residues (…

Promoter

CMV

Available since

July 24, 2013

Availability

Academic Institutions and Nonprofits only

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pRK5-HA GST RagD 77L

Plasmid

#19308

Depositor

David Sabatini

Article

Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

Insert

Ras-related GTP binding D (RRAGD Human)

Use

Tags

GST and HA

Expression

Mammalian

Mutation

S77L

Promoter

Available since

Sept. 15, 2008

Availability

Academic Institutions and Nonprofits only

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pRK5-HA GST RagB 54L

Plasmid

#19302

Depositor

David Sabatini

Article

Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

Insert

Ras-related GTP binding B (RRAGB Human)

Use

Tags

GST and HA

Expression

Mammalian

Mutation

T54L

Promoter

Available since

Sept. 15, 2008

Availability

Academic Institutions and Nonprofits only
pRK5-HA GST RagD 121L

Plasmid

#19309

Depositor

David Sabatini

Article

Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

Insert

Ras-related GTP binding D (RRAGD Human)

Use

Tags

GST and HA

Expression

Mammalian

Mutation

Q121L

Promoter

Available since

Sept. 15, 2008

Availability

Academic Institutions and Nonprofits only

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pRK5-myc-Rac1-Q61L

Plasmid

#12983

Depositor

Gary Bokoch

Insert

Rac1 constitutively active (RAC1 Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

Q61L Constitutively active

Promoter

Available since

Oct. 27, 2006

Availability

Academic Institutions and Nonprofits only

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pRK5-mEGFP-SQSTM1-MUT

Plasmid

#194583

Purpose

Mammalian Expression of mEGFP-SQSTM1 Mutant (V271Sfs*41) Fusion Protein

Depositor

Denes Hnisz

Article

Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

Insert

SQSTM1 (SQSTM1 Human)

Use

Tags

mEGFP

Expression

Mammalian

Mutation

V271Sfs*41

Promoter

Available since

Feb. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pRK5 FLAG human DEPTOR

Plasmid

#21334

Depositor

David Sabatini

Article

Peterson et al Cell. 2009 May 13. ():.

Insert

DEPTOR (DEPTOR Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

N204S and S389N mutations (numbering according to…

Promoter

Available since

June 9, 2009

Availability

Academic Institutions and Nonprofits only

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pRK5-HA GST RagC 120L

Plasmid

#19306

Depositor

David Sabatini

Article

Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

Insert

Ras-related GTP binding C (RRAGC Human)

Use

Tags

GST and HA

Expression

Mammalian

Mutation

Q120L

Promoter

Available since

Sept. 15, 2008

Availability

Academic Institutions and Nonprofits only

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Flag-MIOS(1-875) in pRK5

Plasmid

#184561

Purpose

CMV-driven expression of an N-terminally Flag-tagged MIOS (core subunit of GATOR2) — native sequence for transient expression in mammalian cells.

Depositor

Kacper Rogala

Article

Valenstein et al Nature. 2022 Jul;607(7919):610-616. doi:

Insert

MIOS (MIOS Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pRK5-HA GST RagA 66L

Plasmid

#19300

Depositor

David Sabatini

Article

Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

Insert

Ras-related GTP binding A (RRAGA Human)

Use

Tags

GST and HA

Expression

Mammalian

Mutation

Q66L

Promoter

Available since

Sept. 15, 2008

Availability

Academic Institutions and Nonprofits only

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pRK5 CASTOR1-Flag Cterm

Plasmid

#84488

Purpose

overexpression of CASTOR1 with Flag tag

Depositor

David Sabatini

Article

Chantranupong et al Cell. 2016 Mar 24;165(1):153-64. doi: 10

Insert

CASTOR1 (GATSL3 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 24, 2016

Availability

Academic Institutions and Nonprofits only
pRK5 Flag Grb10 wt long

Plasmid

#37481

Depositor

David Sabatini

Article

Hsu et al Science. 2011 Jun 10;332(6035):1317-22.

Insert

Grb10 (GRB10 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

isoform a, 594aa

Promoter

Available since

Aug. 30, 2012

Availability

Academic Institutions and Nonprofits only

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pRK5-myc-Rac1-T17N

Plasmid

#12984

Depositor

Gary Bokoch

Insert

Rac1 DN (RAC1 Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

T17N Dominant negative

Promoter

Available since

Oct. 27, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pRK5-HA GST RagA 21L

Plasmid

#19299

Depositor

David Sabatini

Article

Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

Insert

Ras-related GTP binding A (RRAGA Human)

Use

Tags

GST and HA

Expression

Mammalian

Mutation

T21L

Promoter

Available since

Sept. 15, 2008

Availability

Academic Institutions and Nonprofits only
[pSK729] pRK5 HA-FLCN(R164A)

Plasmid

#136147

Purpose

mammalian expression of FLCN

Depositor

David Sabatini, Kuang Shen

Article

Shen et al Cell. 2019 Nov 27;179(6):1319-1329.e8. d

Insert

FLCN (FLCN Human)

Use

Tags

HA

Expression

Mammalian

Mutation

R164A

Promoter

Available since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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