Flag-MIOS(1-875) in pRK5
(Plasmid #184561)

• Purpose: CMV-driven expression of an N-terminally Flag-tagged MIOS (core subunit of GATOR2) — native sequence for transient expression in mammalian cells.
• Depositing Lab: Kacper Rogala
• Publication: Valenstein et al Nature. 2022 Jul;607(7919):610-616. doi: 10.1038/s41586-022-04939-z. Epub 2022 Jul 13.

Backbone

• Vector backbone: pRK5
• Backbone manufacturer: Genentech
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: MIOS
• Alt name: Meiosis regulator for oocyte development
• Alt name: Missing oocyte meiosis regulator homolog
• Alt name: GATOR complex protein MIOS
• Species: H. sapiens (human)
• Insert Size (bp): 2628
• GenBank ID: NM_001370076
• Entrez Gene: MIOS (a.k.a. MIO, Sea4, Yulink)
• Promoter: CMV
• Tag / Fusion Protein:
  • Flag (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SalI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: (1) CMV Forward, (2) SP6
• 3′ sequencing primer: EBV Reverse

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry