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pSAH49 - [PVQp | mScarlet | tbb-2 UTR]
Plasmid#200325PurposemScarlet BioPart for PVQ neurons. (1) Mark neurons or (2) cell-specific expression of transgenes (N or C-terminal fusions, or untagged). Compatible with single-copy insertion (MosTI) or arrays.DepositorInsert[PVQp | wrmscarlet | tbb-2 UTR]
UseTagsExpressionWormMutationPromoterWBGene00003755, nlp-17Available sinceJune 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
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pMLS328
Plasmid#73717PurposeC. elegans germline CRE expression vectorDepositorInsert2xNLS-CRE
UseCre/LoxTagsExpressionWormMutationPromoterPeft-3Available sinceMarch 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCCM935
Plasmid#58202PurposeExpresses unc-22 sgRNA in C.elegans. Can be used in Co-CRISPR experiments.DepositorInsertunc-22 sgRNA
UseTagsExpressionWormMutationPromoterU6Available sinceJuly 30, 2014AvailabilityAcademic Institutions and Nonprofits only -
pJH4260
Plasmid#191678PurposePtwk-40s-GCaMP::wScarlet unc-54 3' UTR C.elegans AVA/other neurons expression GCaMP6s wScarletDepositorInsertGCaMP6s
UseTagswScarletExpressionWormMutationPromoterPtwk-40Available sinceNov. 30, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCFJ150-Cas9(dpiRNA)
Plasmid#107940Purposeeft-3p::Cas9(dpiRNA)::tbb-2 3'UTR construct used for MosSCI in nematode. Cas9 is optimized by removing all piRNA targeting sites to allow germline expression.DepositorInsertCas9(dpiRNA)
UseTagsExpressionWormMutationPromotereft-3Available sinceApril 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCM5.37
Plasmid#17253DepositorInsertunc-54 3' from pPD49.78 in pDONRP2RP3
UseGatewayTagsExpressionMutationPromoterAvailable sinceMarch 13, 2008AvailabilityAcademic Institutions and Nonprofits only -
L4440_BioBrick-hif-1-sgRNA-A
Plasmid#177784PurposeOverexpression of sgRNAs in E. coli HT115 (targeting C. elegans hif-1 promoter)DepositorInserthif-1 (hif-1 Nematode)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCFJ353 - MCS(ttTi4391, I)
Plasmid#34869DepositorInsertttTi4391 targeting
UseWorm targetingTagsExpressionMutationPromoterAvailable sinceFeb. 1, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCM1.57
Plasmid#17252DepositorInsertL3788 promoter NO ATG in pDONRP4P1R
UseGatewayTagsExpressionMutationPromoterAvailable sinceFeb. 26, 2008AvailabilityAcademic Institutions and Nonprofits only -
pSEM416 - mosTI unc-119 P_UPN - gfp(nls) - tbb-2
Plasmid#182359PurposePositive control for mosTI(unc-119). unc-119 rescue and pan-neuronal nuclear GFP expression after mosTI insertion.DepositorInsertUltra-pan-neuronal positive control pUNP::gfp(NLS)
UseTagsExpressionWormMutationPromoterPromoter Ultra Pan NeuronalAvailable sinceMay 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJW4.02
Plasmid#62867PurposeBacterial expression of MBP-PGL-1DepositorInsertMBP-PGL-1
UseTagsMBPExpressionBacterialMutationPromoterAvailable sinceMarch 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBN280
Plasmid#86731PurposeTissue-specific FLP expression plasmid for C. elegansDepositorInsertPtph-1::FLP D5
UseTagsExpressionWormMutationPromoterAvailable sinceMarch 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
optGluCl alpha EYFP
Plasmid#15104DepositorInsertGluCl alpha (glc-1 Nematode)
UseTagsEYFPExpressionMammalianMutationCodon optimized cDNAPromoterAvailable sinceJune 8, 2007AvailabilityAcademic Institutions and Nonprofits only -
punc-119cR
Plasmid#21596DepositorInsertunc-119:mCherry (unc-119 Nematode)
UseAdds unc-119 marker to amp resistant plasmidsTagsmCherryExpressionWormMutationN/APromoterAvailable sinceAug. 26, 2009AvailabilityAcademic Institutions and Nonprofits only -
pCZGY2851
Plasmid#87761PurposePunc-17b-PH::miniSOG(Q103L), expression of enhanced mutant version of membrane targeted PH-miniSOG (Q103L) in cholinergic motor neurons.DepositorInsertPunc-17b-PH-miniSOG(Q103L)
UseTagsPHExpressionWormMutationPromoterPunc-17bAvailable sinceApril 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
SNF-5 pXOOM
Plasmid#48697PurposeExpressed SNF-5 in Xenopus oocytes or mammalian cellsDepositorInsertsnf-5 (snf-5 Nematode)
UseXenopus oocyte expressionTagsExpressionMammalianMutationK42E (when compared to NP_496735.1)PromoterT7Available sinceDec. 20, 2013AvailabilityAcademic Institutions and Nonprofits only -
pUA97
Plasmid#69340PurposeIt encodes a fluorescent protein that can be fused to a gene of interest using SEC CRISPR strategyDepositorInsertceBFP SEC 3xFlag
UseTags3xFlag and ceBFP (mTagBFP)ExpressionWormMutationsqt-1(e1350)PromoterAvailable sinceDec. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCM5.47
Plasmid#17254DepositorInsertpie-1 3'utr in pDONRP2RP3
UseGatewayTagsExpressionMutationPromoterAvailable sinceFeb. 26, 2008AvailabilityAcademic Institutions and Nonprofits only
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