pJH4723
(Plasmid
#178899)
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PurposePges-1 DAF-2 unc-54 3' UTR C.elegans gut expression of of DAF-2
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 178899 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 * |
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Backbone
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Vector backbonepBSK
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Backbone manufacturerstratagene
- Backbone size w/o insert (bp) 5900
- Total vector size (bp) 12470
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Vector typeWorm Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameDAF-2
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SpeciesC. elegans (nematode)
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Insert Size (bp)6570
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GenBank ID
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Entrez Genedaf-2 (a.k.a. CELE_Y55D5A.5)
- Promoter Pges-1
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site XmaI (not destroyed)
- 3′ cloning site KpnI (not destroyed)
- 5′ sequencing primer GGATCCcccgggATGAATATTGTCA
- 3′ sequencing primer atccATggtaccACAAGTGGATGCTCA (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pJH4723 was a gift from Mei Zhen (Addgene plasmid # 178899 ; http://n2t.net/addgene:178899 ; RRID:Addgene_178899) -
For your References section:
A Caenorhabditis elegans developmental decision requires insulin signaling-mediated neuron-intestine communication. Hung WL, Wang Y, Chitturi J, Zhen M. Development. 2014 Apr;141(8):1767-79. doi: 10.1242/dev.103846. Epub 2014 Mar 26. 10.1242/dev.103846 PubMed 24671950