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Showing: 381 - 400 of 18488 results

  3. pLKO.1 puro

    Plasmid
    #8453
    Purpose
    3rd gen lentiviral backbone for cloning and expression of new shRNA sequences. Uses puromycin for selection.
    Depositor
    Bob Weinberg
    Article
    Stewart et al RNA 2003 Apr;9(4):493-501.
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    Lentiviral and RNAi
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 18, 2005
    Availability
    Academic Institutions and Nonprofits only
    8453-plasmid-map-sequence-id-421039

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  4. lentiGuide-Puro

    Plasmid
    #52963
    Purpose
    Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and puromycin resistance from EF-1a promoter. 3rd generation lentiviral backbone.
    Depositor
    Feng Zhang
    Article
    Sanjana et al Nat Methods. 2014 Aug;11(8):783-4. doi:
    Has Service
    Cloning Grade DNA
    Inserts
    S. pyogenes sgRNA cassette
    Puromycin resistance
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Ef1-a and hU6
    Available since
    May 8, 2014
    Availability
    Academic Institutions and Nonprofits only
    52963-plasmid-map-sequence-id-331247

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  7. pDRGFP

    Plasmid
    #26475
    Purpose
    In vivo homologous recombination substrate composed of two differentially mutated GFP genes oriented as direct repeats and separated by a drug selection marker
    Depositor
    Maria Jasin
    Article
    Pierce et al Genes Dev. 1999 Oct 15. 13(20):2633-8.
    Insert
    DRGFP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 18, 2010
    Availability
    Academic Institutions and Nonprofits only
    26475-plasmid-map-sequence-id-432928

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  8. pAAV2/2

    Plasmid
    #104963
    Purpose
    AAV packaging plasmid expressing Rep/Cap genes for production of serotype 2
    Depositor
    Melina Fan
    Insert
    Rep2/Cap2
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    p5
    Available since
    Oct. 2, 2018
    Availability
    Industry, Academic Institutions, and Nonprofits
    104963-plasmid-map-sequence-id-372962

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  9. mKeima-Red-Mito-7

    Plasmid
    #56018
    Purpose
    Localization: Mitochondria, Excitation: 440, Emission: 620
    Depositor
    Michael Davidson
    Insert
    Mito (COX8A Human)
    Use
    Tags
    mKeima-Red
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    April 1, 2015
    Availability
    Academic Institutions and Nonprofits only
    56018-plasmid-map-sequence-id-201345

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  12. pJR104

    Plasmid
    #187243
    Purpose
    Lentiviral sgRNA vector with mU6 sgRNA promoter, CR1 constant region, and UCOE EF1alpha driving PURO-GFP marker expression
    Depositor
    Jonathan Weissman, Marco Jost
    Article
    Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75
    Insert
    Lentiviral sgRNA vector with mU6 sgRNA promoter, CR1 constant region, and UCOE EF1alpha driving PURO-GFP marker expression
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 19, 2022
    Availability
    Academic Institutions and Nonprofits only
    187243-plasmid-map-sequence-id-374823

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  14. pdCas9-bacteria

    Plasmid
    #44249
    Purpose
    aTc-inducible expression of a catalytically inactive bacterial Cas9 (S. pyogenes) for bacterial gene knockdown
    Depositor
    Stanley Qi
    Article
    Qi et al Cell. 2013 Feb 28;152(5):1173-83. doi: 1
    Insert
    dCas9 (bacteria)
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    D10A H840A (catalytically inactive)
    Promoter
    pLtetO-1
    Available since
    April 11, 2013
    Availability
    Academic Institutions and Nonprofits only
    44249-plasmid-map-sequence-id-269588

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  15. pCMV-T7 RNA POL

    Plasmid
    #138352
    Purpose
    Expressing T7 RNA Polymerase in mammalian cells
    Depositor
    Baisong Lu
    Insert
    T7 RNA POL
    Use
    CRISPR and Lentiviral
    Tags
    No
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 23, 2022
    Availability
    Academic Institutions and Nonprofits only
    138352-plasmid-map-sequence-id-273271

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  16. SV40 1: pBSSVD2005

    Plasmid
    #21826
    Depositor
    David Ron
    Insert
    SV40 Large T (SV40gp6 simian virus)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    likely expresses aa109-708 of the large T antigen
    Promoter
    Available since
    Sept. 14, 2009
    Availability
    Academic Institutions and Nonprofits only
    21826-plasmid-map-sequence-id-352432

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  18. pLV-TetO-hNGN2-eGFP-Puro

    Plasmid
    #79823
    Purpose
    Expresses human NEUROGENIN2 (hNGN2), eGFP and puromycin resistance gene under control of TetON promoter. This 3rd generation lentiviral vector is used to generate NGN2-iNs from hiPSCs and hiPSC-NPCs.
    Depositor
    Kristen Brennand
    Article
    Ho et al Methods. 2015 Nov 25. pii: S1046-2023(15
    Insert
    hNGN2-P2A-eGFP-T2A-PuroR (NEUROG2 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    TetO
    Available since
    July 21, 2016
    Availability
    Academic Institutions and Nonprofits only
    79823-plasmid-map-sequence-id-240610

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Showing: 381 - 400 of 18488 results