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Showing: 21 - 40 of 16965 results

  1. pMB1610_pRR-Puro

    Plasmid
    #65853
    Purpose
    Puromycin-based homologous recombination-dependent reporter for genome editing with TALENs or CRISPR/Cas9
    Depositor
    Marc Bühler
    Article
    Flemr et al Cell Rep. 2015 Jul 28;12(4):709-16. doi:
    Insert
    split puromycin N-acetyltransferase coding sequence
    Use
    CRISPR and TALEN
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    SV40
    Available since
    Aug. 11, 2015
    Availability
    Academic Institutions and Nonprofits only
    65853-plasmid-map-sequence-id-112015

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  4. Puro-iDEST

    Plasmid
    #75336
    Purpose
    GATEWAY destination vector for site-specific integration into the human AAVS1/PPP1R12C locus for doxycycline-inducible gene over-expression
    Depositor
    Danwei Huangfu
    Article
    Zhu et al Cell Stem Cell. 2016 Apr 27. pii: S1934-
    Insert
    TRE-DEST
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    TRE-tight
    Available since
    June 14, 2016
    Availability
    Academic Institutions and Nonprofits only
    75336-plasmid-map-sequence-id-149881

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  9. pGCGFP-puro

    Plasmid
    #31266
    Depositor
    Andrew Pierce
    Insert
    GCGFP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Sept. 19, 2011
    Availability
    Academic Institutions and Nonprofits only
    31266-plasmid-map-sequence-id-14329

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  10. lentiGuideFB-Puro-A

    Plasmid
    #192506
    Purpose
    For cloning of sgRNAs compatible with PspCas9. Contains a 5' direct repeat and a nd a unique sgRNA scaffold variant with a capture sequence. Cloning guide RNAs using BsmBI.
    Depositor
    Neville Sanjana
    Article
    Wessels et al Nat Methods. 2023 Jan;20(1):86-94. doi:
    Insert
    hU6-sgRNA-CS1-BsmBI-EFS-Puro-WPRE
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    hU6
    Available since
    Oct. 21, 2022
    Availability
    Academic Institutions and Nonprofits only
    192506-plasmid-map-sequence-id-375156

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  11. pSc1-puro

    Plasmid
    #80438
    Purpose
    Expresses eGFP along with an U6 promoter driven gRNA which cleaves the plasmid in-cell, and also confers resistance to puromycin
    Depositor
    Ervin Welker
    Article
    Talas et al DNA Res. 2017 Jun 30. doi: 10.1093/dnare
    Inserts
    sp Cas9 gRNA
    eGFP
    Puromycin resistance
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, U6, and hPGK
    Available since
    Sept. 12, 2016
    Availability
    Academic Institutions and Nonprofits only
    80438-plasmid-map-sequence-id-151453

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  13. pBLIC-puro

    Plasmid
    #45197
    Purpose
    a ligation-independent cloning (LIC) retroviral vector for stable gene transduction in mammalian cells. Puro selection.
    Depositor
    Priyamvada Rai
    Article
    Patel et al BMC Biotechnol. 2012 Jan 16;12:3. doi: 1
    Type
    Empty backbone
    Use
    Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 19, 2015
    Availability
    Academic Institutions and Nonprofits only

  15. pXRDRE-puro

    Plasmid
    #31270
    Depositor
    Andrew Pierce
    Insert
    XRDRE
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Sept. 20, 2011
    Availability
    Academic Institutions and Nonprofits only
    31270-plasmid-map-sequence-id-14333

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  20. pUC19-MAFA-PQR-NLS-tdTomato-P2A-puro-PGK-puro

    Plasmid
    #121174
    Purpose
    Donor template for CRISPR-Cas9 mediated generation of MAFA-NLS-tdTomato reporter cell lines
    Depositor
    Timo Otonkoski
    Insert
    MAFA-PQR-NLS-tdTomato-P2A-Puro-PGK-Puro
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    121174-plasmid-map-sequence-id-234993

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Showing: 21 - 40 of 16965 results