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Plasmid#111149PurposeExpresses arginylated human beta-actin fused with thymosin beta and a His tag.DepositorInsertACTB (ACTB Human)
UsePichia pastoris integration plasmidTagsThymosin beta and a His-tagExpressionYeastMutationN-terminal arginylationPromoterAOX1Available sinceJune 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pPICZ A-SLC25A46[∆2-83]-His (SB258)
Plasmid#227607PurposeInducible expression of His-tagged SLC25A46 lacking its N-terminal region (∆2-83) in Pichia pastorisDepositorInsertSLC25A46 (SLC25A46 Human)
UseTags10xHisExpressionYeastMutationaa 2-83 deletedPromoterAOX1Available sinceDec. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPICZ A-SLC25A46-His (SB250)
Plasmid#227599PurposeInducible expression of His-tagged SLC25A46 in Pichia pastorisDepositorInsertSLC25A46 (SLC25A46 Human)
UseTags10xHisExpressionYeastMutationPromoterAOX1Available sinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPICZ A-Strep-Opa1 (SB251)
Plasmid#227600PurposeInducible expression of Strep-tagged Opa1 in Pichia pastorisDepositorInsertOPA1 (OPA1 Human)
UseTagsTwin-StrepTagExpressionYeastMutationcontains aa 253-960 onlyPromoterAOX1Available sinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPICZ A-MFN2-Strep (SB253)
Plasmid#227602PurposeInducible expression of Strep-tagged MFN2 in Pichia pastorisDepositorInsertMFN2 (MFN2 Human)
UseTagsTwin-StrepTag, 10xHisExpressionYeastMutationPromoterAOX1Available sinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPICZ:K2P2.1(mTREK-1)Cryst S112C
Plasmid#224780PurposePichia pastoris expression vector for generating mouse TREK-1(21S-322T) + 3C cleavable site + eGFP + 10x His under the AOX1 promoterDepositorInsertPotassium channel subfamily K member 2 (Kcnk2 Mouse)
UseTagsExpressionYeastMutationK84R / Q85E / T86K / I88L / A89R / Q90A / A92P / …PromoterAOX1 promoterAvailable sinceSept. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPICZ-pre-Ost1-alphaf(I)-E2-Crimson
Plasmid#117661PurposeTest intracellular localisation of E2-Crimson and study the secretion efficiencyDepositorInsertpre-Ost1-alphaf(I)-E2-Crimson
UseTagsExpressionYeastMutationThis plasmid encodes the fluorescent protein E2-C…PromoterpAOX1Available sinceDec. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pPICZ-pre-pro-alphaf-Btl2
Plasmid#117664PurposeStudy secretion efficiency of Btl2DepositorInsertpre-pro-alphaf-Btl2
UseTagsExpressionYeastMutationThis plasmid encodes the lipase Btl2 together wit…PromoterpAOX1Available sinceFeb. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPICZ-pre-pro-alphaf(I)-E2-Crimson
Plasmid#117660PurposeTest intracellular localisation of E2-Crimson and study the secretion efficiencyDepositorInsertpre-pro-alphaf(I)-E2-Crimson
UseTagsExpressionYeastMutationThis plasmid encodes the fluorescent protein E2-C…PromoterpAOX1Available sinceDec. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pPICZ-pre-pro-alphaf-E2-Crimson
Plasmid#117658PurposeTest intracellular localisation of E2-Crimson and study the secretion efficiencyDepositorInsertpre-pro-alphaf-E2-Crimson
UseTagsExpressionYeastMutationThis plasmid encodes the fluorescent protein E2-C…PromoterpAOX1Available sinceDec. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pPICZ-mouseGIRK2-eGFP-StII-6xHis
Plasmid#124277PurposeExpresses mouse GIRK2 as a TEV protease cleavable C-terminal fusion to eGFP, strep tag II, and 6x His tagDepositorInsertGIRK2 (Kcnj6 Mouse)
UseTagseGFP-StreptagII-6xHisExpressionYeastMutationPromoterAOX1Available sinceApril 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPICZ-mouseGIRK2R201A-eGFP-StII-6xHis
Plasmid#124278PurposeExpresses mouse GIRK2 R201A as a TEV protease cleavable C-terminal fusion to eGFP, strep tag II, and 6x His tagDepositorInsertGIRK2 (Kcnj6 Mouse)
UseTagseGFP-StreptagII-6xHisExpressionYeastMutationR201APromoterAOX1Available sinceApril 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPICZ-pre-Ost1-alphaf(I)-Btl2
Plasmid#117667PurposeStudy secretion efficiency of Btl2DepositorInsertpre-Ost1-alphaf(I)-Btl2
UseTagsExpressionYeastMutationThis plasmid encodes the lipase Btl2 together wit…PromoterpAOX1Available sinceDec. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pPICZ A-SLC25A46[I349D]-His (SB256)
Plasmid#227605PurposeInducible expression of His-tagged SLC25A46 with I349D mutation in Pichia pastorisDepositorInsertSLC25A46 (SLC25A46 Human)
UseTags10xHisExpressionYeastMutationI349DPromoterAOX1Available sinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPICZ A-Strep-Opa1(MGD) (SB252)
Plasmid#227601PurposeInducible expression of Strep-tagged Opa1(MGD) in Pichia pastorisDepositorInsertOPA1 (OPA1 Human)
UseTagsTwin-StrepTagExpressionYeastMutationcontains aa 253-580 (MGD) and aa 938-960PromoterAOX1Available sinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPICZ-His6-K2P4.1A-mCherry-StreptagII
Plasmid#158743PurposeExpresses K2P4.1 isoform A with TEV protease cleavable N- and C-terminal affinity tags.DepositorInsertK2P4.1-A (KCNK4 Human)
UseTagsHis6 and mCherry with Streptag IIExpressionYeastMutationResidues 1-264 with N-linked glycosylation sites …PromoterAOX1Available sinceSept. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPICZ-His6-K2P4.1B-mCherry-StreptagII
Plasmid#158744PurposeExpresses K2P4.1 isoform B with TEV protease cleavable N- and C-terminal affinity tags.DepositorInsertK2P4.1-B (KCNK4 Human)
UseTagsHis6 and mCherry with Streptag IIExpressionYeastMutationResidues 1-290 and N-linked glycosylation sites r…PromoterAOX1Available sinceSept. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPICZ-pOst1-pro-alphaf(MUT1)-E2-Crimson
Plasmid#117662PurposeTest intracellular localisation of E2-Crimson and study the secretion efficiencyDepositorInsertpOst1-pro-af(MUT1)-E2-Crimson
UseTagsExpressionYeastMutationThis plasmid encodes the fluorescent protein E2-C…PromoterpAOX1Available sinceDec. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pPICZ A-SLC-His+Strep-Opa1 (SB254)
Plasmid#227603PurposeInducible coexpression of His-tagged SLC25A46 and Strep-tagged Opa1 in Pichia pastorisDepositorInsertsUseTags10xHis and Twin-StrepTagExpressionYeastMutationcontains aa 253-960 onlyPromoterAOX1Available sinceDec. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPICZ A-SLC[∆2-83]-His+MFN2-Strep (SB259)
Plasmid#227608PurposeInducible coexpression of His-tagged SLC25A46 in its N-terminal region (∆2-83) and Strep-tagged MFN2 in Pichia pastorisDepositorUseTags10xHis and Twin-StrepTagExpressionYeastMutationaa 2-83 deletedPromoterAOX1Available sinceDec. 6, 2024AvailabilityAcademic Institutions and Nonprofits only