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Plasmid#167934PurposeAll-in-one lentiviral CRISPRa gRNA expression with blasticidin selection marker.DepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6, EF1aAvailable sinceMay 7, 2021AvailabilityAcademic Institutions and Nonprofits only
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p65-mNeonGreen
Plasmid#127172PurposeFluorescently tagged p65 reporterDepositorInsertGFP-RelA (RELA Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceAug. 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
LentiGuide-Hygro
Plasmid#139462PurposeLentiviral vector with gRNA scaffold and hygromycin selectable markerDepositorInsertno sgRNA inserted; resistance gene: hygroR
UseLentiviralTagsExpressionMammalianMutationPromoterEF-1a / U6Available sinceAug. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
mRNA-CleanCap-NanoLuc
Plasmid#188247PurposeTemplate plasmid to transcribe mRNA encoding NanoLucDepositorInsertNanoLuc
UseLuciferaseTagsExpressionMammalianMutationPromoterT7Available sinceSept. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCDH-CMV-CD19 puro
Plasmid#196634PurposeExpress CD19DepositorInsertCD19 (CD19 Human)
UseLentiviralTagsExpressionMutationWTPromoterAvailable sinceFeb. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV.CAG.(mito).cpSFGFP.HaloTag
Plasmid#214925PurposeExpresses mitochondrially targeted non-responsive controlDepositorInsert(mito).cpSFGFP.HaloTag
UseAAVTagsExpressionMammalianMutationPromoterCAGAvailable sinceApril 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
LV_EF1a_SV40_Large_T_Antigen-P2A-Hygro_Barcode
Plasmid#170255PurposeBarcoded lentiviral vector to express SV40 Large T Antigen in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seqDepositorInsertSV40 Large T Antigen
UseLentiviralTagsExpressionMutationPromoterEF1aAvailable sinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRC0569 (parts entry vector)
Plasmid#189966PurposeCloning vector for generating part plasmids, with mNeonGreen dropout following BsmBI digestionDepositorInsertmNeonGreen
UseTagsExpressionMammalianMutationPromoterBBa-J23100Available sinceSept. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHAGE2_EF1a_TetOn_IRES_mCherry
Plasmid#204155PurposeLentivirus backbone expressing rtTA linked to mCherryDepositorInsertTet-On 3G
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceAug. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
p-dCas9-JMJD2A-Hygro
Plasmid#104410Purposetransient expression of dCas9-JMJD2A fusion proteinDepositorInsertJMJD2A (KDM4A Human)
UseCRISPRTagsExpressionMammalianMutationPromoterCMV promoterAvailable sinceApril 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRosa26-GT
Plasmid#40025DepositorInsertsGFP
beta-globin intron
Neo
tdT-3Myc
diphteria toxin A
UseTags3 Myc tagsExpressionMammalianMutationdeleted nucleotides after nucleotide 274, inserti…PromoterCAG (chicken beta globin promoter and CMV enhance…Available sinceOct. 22, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCMVd1-EGFP-hRhoA-wt
Plasmid#133926PurposeLow level expression of GFP fused human RhoA wild typeDepositorInsertGFP-hRhoA WT (RHOA Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
lentiGuide-Puro
Plasmid#52963PurposeExpresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and puromycin resistance from EF-1a promoter. 3rd generation lentiviral backbone.DepositorHas ServiceCloning Grade DNAInsertsS. pyogenes sgRNA cassette
Puromycin resistance
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEf1-a and hU6Available sinceMay 8, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCE-mp53DD
Plasmid#41856PurposeNon-integrating (episomal) expression of mouse p53DD - p53 carboxy-terminal dominant-negative fragmentDepositorInsertTrp53 (Trp53 Mouse)
UseTagsExpressionMammalianMutationΔ40-903PromoterCAGAvailable sinceJuly 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAAV.CMV.PI.EGFP.WPRE.bGH
Plasmid#105530PurposeAAV expression of EGFP from CMV promoterDepositorHas ServiceAAV PHP.eB, AAV rh10, AAV1, AAV2, AAV5, AAV8, and AAV9InsertEGFP
UseAAVTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
CROP-seq-opti
Plasmid#106280PurposeA version of the CROP-seq plasmid as presented in Datlinger et al. that contains the sgRNA-(F+E)-combined optimized backbone for CRISPRi from Chen et al.DepositorInsertEF1a-Puro-WPRE-hU6-gRNA
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceMarch 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pdCas9-bacteria
Plasmid#44249PurposeaTc-inducible expression of a catalytically inactive bacterial Cas9 (S. pyogenes) for bacterial gene knockdownDepositorInsertdCas9 (bacteria)
UseCRISPRTagsExpressionBacterialMutationD10A H840A (catalytically inactive)PromoterpLtetO-1Available sinceApril 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
CLYBL-TO-hNGN2-BSD-mApple
Plasmid#124229PurposeTargets the human CLYBL safe harbor locus for integration of dox-inducible NGN2 for differentiation of iPSCs into cortical neurons. Contains a floxed BSD-NLS-mApple selection cassette.DepositorInsertNGN2 (NEUROG2 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-hGal3
Plasmid#73080PurposeExpression in mammalian cellsDepositorInsertGalectin-3 (LGALS3 Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
mRNA-CleanCap-mNeonGreen
Plasmid#188248PurposeTemplate plasmid to transcribe mRNA encoding mNeonGreenDepositorInsertmNeonGreen
UseTagsExpressionMammalianMutationPromoterT7Available sinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
PGC-1 alpha promoter 2kb luciferase
Plasmid#8887DepositorInsertPGC-1 alpha promoter (Ppargc1a Mouse)
UseLuciferaseTagsluciferaseExpressionMammalianMutationPromoterAvailable sinceJan. 5, 2006AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-EGFP
Plasmid#13031PurposeMammalian expression vector for cloning your gene fused to EGFPDepositorHas ServiceCloning Grade DNAInsertEnhanced Green Fluorescent Protein
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 3, 2006AvailabilityAcademic Institutions and Nonprofits only -
pEThT-RvLEAM
Plasmid#90033PurposeTo express His-tagged RvLEAM in bacteriaDepositorInsertRvLEAM
UseTagsHisExpressionBacterialMutationPromoterT7Available sinceMay 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pInducer20 Cyclin E1
Plasmid#109348Purposevirus packaged, doxycyline inducible expression of Cyclin E1DepositorInsertCyclin E1 (CCNE1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJuly 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCAG-R2Tg_opt-3xFlag
Plasmid#223251PurposeMammalian expression of C-terminally Flag-tagged optimized R2Tg proteinDepositorInsertR2Tg protein_opt
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceAug. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTRIP-SFFV-Hygro-2A-TMRPSS2
Plasmid#170390PurposeGeneration of stable lines to enhance SARS-CoV-2 infectivity in ACE2 expressing cellsDepositorInsertTMPRSS2 (TMPRSS2 Human)
UseLentiviralTagsExpressionMutationPromoterSFFVAvailable sinceJune 23, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
AICSDP-26:ST6GAL1-mEGFP
Plasmid#101786PurposeHomology arms and linker-mEGFP sequence for C-terminus tagging of human ST6GAL1DepositorInsertST6GAL1 Homology Arms with linker-mEGFP (ST6GAL1 Human)
UseCRISPR; Donor templateTagslinker-mEGFPExpressionMammalianMutationhomology arms contain point mutations to disrupt …PromoterAvailable sinceOct. 31, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
pTRIP-GFP-IRF3
Plasmid#127663PurposeOver-express EGFP-IRF3DepositorInsertIRF3 (IRF3 Human)
UseLentiviralTagsEGFPExpressionMutationPromoterAvailable sinceJuly 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCHAC-mt-mKeima
Plasmid#72342Purposeretrovirus construct for stable expressing mt-mKeimaDepositorInsertmt-mKeima
UseRetroviralTagsExpressionMutationPromoterAvailable sinceJan. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -